Preparation and characterization of Arctium lappa L. polysaccharide-Zn(II) complex and evaluation of its immune activity
A novel Arctium lappa L. polysaccharide zinc (ALP-Zn) complex was synthesized with success. The structural characteristics, stability, and in vitro digestive properties of ALP-Zn were studied. Moreover, the immunoreactivity of ALP-Zn on murine bone marrow-derived dendritic cells (BMDCs) was investig...
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| Language: | English |
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Elsevier
2024-12-01
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| Series: | Food Chemistry Advances |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772753X24001801 |
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| author | Yang Liu Wanbing Pan Lingyu Li Jianquan Gong Hongjing Dong Bin Zhang Jiahao Lin Zhenjia Zheng |
| author_facet | Yang Liu Wanbing Pan Lingyu Li Jianquan Gong Hongjing Dong Bin Zhang Jiahao Lin Zhenjia Zheng |
| author_sort | Yang Liu |
| collection | DOAJ |
| description | A novel Arctium lappa L. polysaccharide zinc (ALP-Zn) complex was synthesized with success. The structural characteristics, stability, and in vitro digestive properties of ALP-Zn were studied. Moreover, the immunoreactivity of ALP-Zn on murine bone marrow-derived dendritic cells (BMDCs) was investigated by examining surface molecule expression, cytokine production and endocytosis. The results showed that the weight-average molecular weight (Mw) of ALP-Zn was 60,268 Da. ALP-Zn mainly consisted of fructose, arabinose, galactose, and glucose in a mass ratio of 50.74:27.94:12.49:7.26. Zn-O characteristic peak was identified by fourier transform infrared spectroscopy at 433.42 cm−1, indicating that zinc was successfully complexed with ALP. The stability and in vitro digestive properties suggested that ALP-Zn had good stability and biological acceptability. Furthermore, ALP and ALP-Zn demonstrated excellent immunological effects on dendritic cells (DCs). After being treated with ALP and ALP-Zn, CD80 and CD86 expression on the surface of DCs were increased and DCs maturation was induced. Moreover, ALP and ALP-Zn also significantly reduced DC endocytosis and increased cytokine (IFN-γ, TNF-α, IL-1β and IL-6). As a consequence, ALP-Zn complex could be as a potential zinc supplement with immune properties. |
| format | Article |
| id | doaj-art-e1b546026a584271afe600d0e47c3981 |
| institution | OA Journals |
| issn | 2772-753X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry Advances |
| spelling | doaj-art-e1b546026a584271afe600d0e47c39812025-08-20T01:59:35ZengElsevierFood Chemistry Advances2772-753X2024-12-01510078410.1016/j.focha.2024.100784Preparation and characterization of Arctium lappa L. polysaccharide-Zn(II) complex and evaluation of its immune activityYang Liu0Wanbing Pan1Lingyu Li2Jianquan Gong3Hongjing Dong4Bin Zhang5Jiahao Lin6Zhenjia Zheng7Key Laboratory of Food Nutrition and Healthy in Universities of Shandong, College of Food Science and Engineering, Shandong Agricultural University, 61 Daizong Street, Tai'an 271018, Shandong, PR ChinaThe Clinical Department, College of Veterinary Medicine, China Agricultural University, 2 Yuanming Yuan Western Road, Beijing 100193, PR China; Center of Research and Innovation of Chinese Traditional Veterinary Medicine, China Agricultural University, 2 Yuanming Yuan Western Road, Beijing 100193, PR ChinaKey Laboratory of Food Nutrition and Healthy in Universities of Shandong, College of Food Science and Engineering, Shandong Agricultural University, 61 Daizong Street, Tai'an 271018, Shandong, PR ChinaYantai Baimaishan Biotechnology Co., Ltd., 3 Fushan Street, Yantai 265200, Shandong, PR ChinaShandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, Shandong, PR ChinaKey Laboratory of Food Nutrition and Healthy in Universities of Shandong, College of Food Science and Engineering, Shandong Agricultural University, 61 Daizong Street, Tai'an 271018, Shandong, PR ChinaThe Clinical Department, College of Veterinary Medicine, China Agricultural University, 2 Yuanming Yuan Western Road, Beijing 100193, PR China; Center of Research and Innovation of Chinese Traditional Veterinary Medicine, China Agricultural University, 2 Yuanming Yuan Western Road, Beijing 100193, PR China; Corresponding authors.Key Laboratory of Food Nutrition and Healthy in Universities of Shandong, College of Food Science and Engineering, Shandong Agricultural University, 61 Daizong Street, Tai'an 271018, Shandong, PR China; Corresponding authors.A novel Arctium lappa L. polysaccharide zinc (ALP-Zn) complex was synthesized with success. The structural characteristics, stability, and in vitro digestive properties of ALP-Zn were studied. Moreover, the immunoreactivity of ALP-Zn on murine bone marrow-derived dendritic cells (BMDCs) was investigated by examining surface molecule expression, cytokine production and endocytosis. The results showed that the weight-average molecular weight (Mw) of ALP-Zn was 60,268 Da. ALP-Zn mainly consisted of fructose, arabinose, galactose, and glucose in a mass ratio of 50.74:27.94:12.49:7.26. Zn-O characteristic peak was identified by fourier transform infrared spectroscopy at 433.42 cm−1, indicating that zinc was successfully complexed with ALP. The stability and in vitro digestive properties suggested that ALP-Zn had good stability and biological acceptability. Furthermore, ALP and ALP-Zn demonstrated excellent immunological effects on dendritic cells (DCs). After being treated with ALP and ALP-Zn, CD80 and CD86 expression on the surface of DCs were increased and DCs maturation was induced. Moreover, ALP and ALP-Zn also significantly reduced DC endocytosis and increased cytokine (IFN-γ, TNF-α, IL-1β and IL-6). As a consequence, ALP-Zn complex could be as a potential zinc supplement with immune properties.http://www.sciencedirect.com/science/article/pii/S2772753X24001801Arctium lappa L.PolysaccharidesZincStructural characterizationImmune activity |
| spellingShingle | Yang Liu Wanbing Pan Lingyu Li Jianquan Gong Hongjing Dong Bin Zhang Jiahao Lin Zhenjia Zheng Preparation and characterization of Arctium lappa L. polysaccharide-Zn(II) complex and evaluation of its immune activity Food Chemistry Advances Arctium lappa L. Polysaccharides Zinc Structural characterization Immune activity |
| title | Preparation and characterization of Arctium lappa L. polysaccharide-Zn(II) complex and evaluation of its immune activity |
| title_full | Preparation and characterization of Arctium lappa L. polysaccharide-Zn(II) complex and evaluation of its immune activity |
| title_fullStr | Preparation and characterization of Arctium lappa L. polysaccharide-Zn(II) complex and evaluation of its immune activity |
| title_full_unstemmed | Preparation and characterization of Arctium lappa L. polysaccharide-Zn(II) complex and evaluation of its immune activity |
| title_short | Preparation and characterization of Arctium lappa L. polysaccharide-Zn(II) complex and evaluation of its immune activity |
| title_sort | preparation and characterization of arctium lappa l polysaccharide zn ii complex and evaluation of its immune activity |
| topic | Arctium lappa L. Polysaccharides Zinc Structural characterization Immune activity |
| url | http://www.sciencedirect.com/science/article/pii/S2772753X24001801 |
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