Prevalence of resistant Staphylococcus aureus strains in frozen meat and their control using phytosynthesized selenium nanoparticles
Staphylococcus aureus continually threaten the safety of meat products, especially the multidrug-resistant (MDR) strains. The screening of MDR S. aureus prevalence in marketed meat products in Saudi Arabia was conducted via molecular identification of resistance genes (cfr, gyrA and gyrB) occurred...
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Elsevier
2021-08-01
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| Series: | Kuwait Journal of Science |
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| Online Access: | https://journalskuwait.org/kjs/index.php/KJS/article/view/10650 |
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| author | Mohammed Sheikh Alsaggaf |
| author_facet | Mohammed Sheikh Alsaggaf |
| author_sort | Mohammed Sheikh Alsaggaf |
| collection | DOAJ |
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Staphylococcus aureus continually threaten the safety of meat products, especially the multidrug-resistant (MDR) strains. The screening of MDR S. aureus prevalence in marketed meat products in Saudi Arabia was conducted via molecular identification of resistance genes (cfr, gyrA and gyrB) occurred in isolated bacteria. The green phytosynthesis of selenium nanoparticles (Se-NPs) was also conducted, using the fruits’ extract of Phyllanthus emblica (PeE), for their evaluation as antibacterial nanocomposites against MDR S. aureus isolates. The prevalence percentage (PP) of S. aureus in meat samples was 14.2%, where the MDR S. aureus prevalence was 9.2%. The highest prevalence of S. aureus isolates were attained from minced meat samples, whereas the highest PP for multidrug-resistant (MDR) S. aureus was recorded from sausages samples. The PeE-synthesized Se-NPs had negative charges, spherical sahpes and well-disperssion with mean diameters of 11.98 nm. The anti- S. aureus activities of PeE, phytosynthesized Se-NPs and their composite (PeE/Se-NPs) were proved qualitatively and quantitatively against different standard and MDR strains, the antibacterial action of PeE/Se-NPs was the strongest. The treatment of MDR S. aureus with PeE/Se-NPs led to severe cells’ lyses/explosion after 8 h of exposure. The nanocomposites from PeE/Se-NPs are recommended for controlling MDR S. aureus in meat.
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| format | Article |
| id | doaj-art-e1b2b6f859b34b238d62c9cfcf42ceb2 |
| institution | OA Journals |
| issn | 2307-4108 2307-4116 |
| language | English |
| publishDate | 2021-08-01 |
| publisher | Elsevier |
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| series | Kuwait Journal of Science |
| spelling | doaj-art-e1b2b6f859b34b238d62c9cfcf42ceb22025-08-20T02:03:36ZengElsevierKuwait Journal of Science2307-41082307-41162021-08-0148410.48129/kjs.v48i4.10650Prevalence of resistant Staphylococcus aureus strains in frozen meat and their control using phytosynthesized selenium nanoparticlesMohammed Sheikh Alsaggaf0College of Science and Humanitarian Studies, Shaqra University, Qwaieah 11971, Saudi Arabia Staphylococcus aureus continually threaten the safety of meat products, especially the multidrug-resistant (MDR) strains. The screening of MDR S. aureus prevalence in marketed meat products in Saudi Arabia was conducted via molecular identification of resistance genes (cfr, gyrA and gyrB) occurred in isolated bacteria. The green phytosynthesis of selenium nanoparticles (Se-NPs) was also conducted, using the fruits’ extract of Phyllanthus emblica (PeE), for their evaluation as antibacterial nanocomposites against MDR S. aureus isolates. The prevalence percentage (PP) of S. aureus in meat samples was 14.2%, where the MDR S. aureus prevalence was 9.2%. The highest prevalence of S. aureus isolates were attained from minced meat samples, whereas the highest PP for multidrug-resistant (MDR) S. aureus was recorded from sausages samples. The PeE-synthesized Se-NPs had negative charges, spherical sahpes and well-disperssion with mean diameters of 11.98 nm. The anti- S. aureus activities of PeE, phytosynthesized Se-NPs and their composite (PeE/Se-NPs) were proved qualitatively and quantitatively against different standard and MDR strains, the antibacterial action of PeE/Se-NPs was the strongest. The treatment of MDR S. aureus with PeE/Se-NPs led to severe cells’ lyses/explosion after 8 h of exposure. The nanocomposites from PeE/Se-NPs are recommended for controlling MDR S. aureus in meat. https://journalskuwait.org/kjs/index.php/KJS/article/view/10650Green synthesisMultidrug-resistanceMode of actionStaphylococcus Prevalence |
| spellingShingle | Mohammed Sheikh Alsaggaf Prevalence of resistant Staphylococcus aureus strains in frozen meat and their control using phytosynthesized selenium nanoparticles Kuwait Journal of Science Green synthesis Multidrug-resistance Mode of action Staphylococcus Prevalence |
| title | Prevalence of resistant Staphylococcus aureus strains in frozen meat and their control using phytosynthesized selenium nanoparticles |
| title_full | Prevalence of resistant Staphylococcus aureus strains in frozen meat and their control using phytosynthesized selenium nanoparticles |
| title_fullStr | Prevalence of resistant Staphylococcus aureus strains in frozen meat and their control using phytosynthesized selenium nanoparticles |
| title_full_unstemmed | Prevalence of resistant Staphylococcus aureus strains in frozen meat and their control using phytosynthesized selenium nanoparticles |
| title_short | Prevalence of resistant Staphylococcus aureus strains in frozen meat and their control using phytosynthesized selenium nanoparticles |
| title_sort | prevalence of resistant staphylococcus aureus strains in frozen meat and their control using phytosynthesized selenium nanoparticles |
| topic | Green synthesis Multidrug-resistance Mode of action Staphylococcus Prevalence |
| url | https://journalskuwait.org/kjs/index.php/KJS/article/view/10650 |
| work_keys_str_mv | AT mohammedsheikhalsaggaf prevalenceofresistantstaphylococcusaureusstrainsinfrozenmeatandtheircontrolusingphytosynthesizedseleniumnanoparticles |