Interleukin-6 from Adipose-Derived Stem Cells Promotes Tissue Repair by the Increase of Cell Proliferation and Hair Follicles in Ischemia/Reperfusion-Treated Skin Flaps
The most common postoperative complication after reconstructive surgery is flap necrosis. Adipose-derived stem cells (ADSCs) and their secretomes are reported to mediate skin repair. This study was designed to investigate whether conditioned media from ADSCs (ADSC-CM) protects ischemia/reperfusion-...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2019/2343867 |
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| author | Chi-Ming Pu Ya-Chun Chen Yu-Chen Chen Tzu-Lin Lee Yu-Sen Peng Shun-Hua Chen Yu-Hsiu Yen Chung-Liang Chien Jung-Hsien Hsieh Yuh-Lien Chen |
| author_facet | Chi-Ming Pu Ya-Chun Chen Yu-Chen Chen Tzu-Lin Lee Yu-Sen Peng Shun-Hua Chen Yu-Hsiu Yen Chung-Liang Chien Jung-Hsien Hsieh Yuh-Lien Chen |
| author_sort | Chi-Ming Pu |
| collection | DOAJ |
| description | The most common postoperative complication after reconstructive surgery is flap necrosis. Adipose-derived stem cells (ADSCs) and their secretomes are reported to mediate skin repair. This study was designed to investigate whether conditioned media from ADSCs (ADSC-CM) protects ischemia/reperfusion- (I/R-) induced injury in skin flaps by promoting cell proliferation and increasing the number of hair follicles. The mouse flap model of ischemia was ligating the long thoracic vessels for 3 h, followed by blood reperfusion. ADSC-CM was administered to the flaps, and their survival was observed on postoperative day 5. ADSC-CM treatment led to a significant increase in cell proliferation and the number of hair follicles. IL-6 levels in the lysate and CM from ADSCs were significantly higher than those from Hs68 fibroblasts. Furthermore, a strong decrease in cell proliferation and the number of hair follicles was observed after treatment with IL-6-neutralizing antibodies or si-IL-6-ADSC. In addition, ADSC transplantation increased flap repair, cell proliferation, and hair follicle number in I/R injury of IL-6-knockout mice. In conclusion, IL-6 secreted from ADSCs promotes the survival of I/R-induced flaps by increasing cell proliferation and the number of hair follicles. ADSCs represent a promising therapy for preventing skin flap necrosis following reconstructive and plastic surgery. |
| format | Article |
| id | doaj-art-e1ad793b40884321a5d976e343d6582a |
| institution | DOAJ |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-e1ad793b40884321a5d976e343d6582a2025-08-20T03:20:59ZengWileyMediators of Inflammation0962-93511466-18612019-01-01201910.1155/2019/23438672343867Interleukin-6 from Adipose-Derived Stem Cells Promotes Tissue Repair by the Increase of Cell Proliferation and Hair Follicles in Ischemia/Reperfusion-Treated Skin FlapsChi-Ming Pu0Ya-Chun Chen1Yu-Chen Chen2Tzu-Lin Lee3Yu-Sen Peng4Shun-Hua Chen5Yu-Hsiu Yen6Chung-Liang Chien7Jung-Hsien Hsieh8Yuh-Lien Chen9Department of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei, TaiwanDivision of Nephrology, Department of Internal Medicine, Far-Eastern Memorial Hospital, New Taipei City, TaiwanDepartment of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, TaiwanDepartment of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei, TaiwanDivision of Plastic Surgery, Department of Surgery, National Taiwan University Hospital, Taipei, TaiwanDepartment of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei, TaiwanThe most common postoperative complication after reconstructive surgery is flap necrosis. Adipose-derived stem cells (ADSCs) and their secretomes are reported to mediate skin repair. This study was designed to investigate whether conditioned media from ADSCs (ADSC-CM) protects ischemia/reperfusion- (I/R-) induced injury in skin flaps by promoting cell proliferation and increasing the number of hair follicles. The mouse flap model of ischemia was ligating the long thoracic vessels for 3 h, followed by blood reperfusion. ADSC-CM was administered to the flaps, and their survival was observed on postoperative day 5. ADSC-CM treatment led to a significant increase in cell proliferation and the number of hair follicles. IL-6 levels in the lysate and CM from ADSCs were significantly higher than those from Hs68 fibroblasts. Furthermore, a strong decrease in cell proliferation and the number of hair follicles was observed after treatment with IL-6-neutralizing antibodies or si-IL-6-ADSC. In addition, ADSC transplantation increased flap repair, cell proliferation, and hair follicle number in I/R injury of IL-6-knockout mice. In conclusion, IL-6 secreted from ADSCs promotes the survival of I/R-induced flaps by increasing cell proliferation and the number of hair follicles. ADSCs represent a promising therapy for preventing skin flap necrosis following reconstructive and plastic surgery.http://dx.doi.org/10.1155/2019/2343867 |
| spellingShingle | Chi-Ming Pu Ya-Chun Chen Yu-Chen Chen Tzu-Lin Lee Yu-Sen Peng Shun-Hua Chen Yu-Hsiu Yen Chung-Liang Chien Jung-Hsien Hsieh Yuh-Lien Chen Interleukin-6 from Adipose-Derived Stem Cells Promotes Tissue Repair by the Increase of Cell Proliferation and Hair Follicles in Ischemia/Reperfusion-Treated Skin Flaps Mediators of Inflammation |
| title | Interleukin-6 from Adipose-Derived Stem Cells Promotes Tissue Repair by the Increase of Cell Proliferation and Hair Follicles in Ischemia/Reperfusion-Treated Skin Flaps |
| title_full | Interleukin-6 from Adipose-Derived Stem Cells Promotes Tissue Repair by the Increase of Cell Proliferation and Hair Follicles in Ischemia/Reperfusion-Treated Skin Flaps |
| title_fullStr | Interleukin-6 from Adipose-Derived Stem Cells Promotes Tissue Repair by the Increase of Cell Proliferation and Hair Follicles in Ischemia/Reperfusion-Treated Skin Flaps |
| title_full_unstemmed | Interleukin-6 from Adipose-Derived Stem Cells Promotes Tissue Repair by the Increase of Cell Proliferation and Hair Follicles in Ischemia/Reperfusion-Treated Skin Flaps |
| title_short | Interleukin-6 from Adipose-Derived Stem Cells Promotes Tissue Repair by the Increase of Cell Proliferation and Hair Follicles in Ischemia/Reperfusion-Treated Skin Flaps |
| title_sort | interleukin 6 from adipose derived stem cells promotes tissue repair by the increase of cell proliferation and hair follicles in ischemia reperfusion treated skin flaps |
| url | http://dx.doi.org/10.1155/2019/2343867 |
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