Cooling Rate Measurement and System Optimization for Cryotop Device

Cryotop is one of the best effective methods for oocytes cryopreservation. When Cryotop is inserted into liquid nitrogen, the cooling speed is extremely high, which is difficult to measure by conventional method. In this study, digital oscilloscopes and type T thermocouple of 25 μm diameter were emp...

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Bibliographic Details
Main Authors: Li Weijie, Zhou Xinli, Dai Jianjun, Zhang Defu, Liu Baolin, Wang Haisong
Format: Article
Language:zho
Published: Journal of Refrigeration Magazines Agency Co., Ltd. 2013-01-01
Series:Zhileng xuebao
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Online Access:http://www.zhilengxuebao.com/thesisDetails#10.3969/j.issn.0253-4339.2013.05.095
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Summary:Cryotop is one of the best effective methods for oocytes cryopreservation. When Cryotop is inserted into liquid nitrogen, the cooling speed is extremely high, which is difficult to measure by conventional method. In this study, digital oscilloscopes and type T thermocouple of 25 μm diameter were employed to establish a high speed temperature measurement system. The cooling rates of Cryotop device at different carrier material, thickness and cold source temperature were measured. The results showed that when 60μm copper was used as carrier and slush nitrogen was used as cold source, the cooling rate was increased. It could reach 37130 ±1336 K/min, which was twice higher than that of commercially available Cryotop (11982 ± 1936 K/min).
ISSN:0253-4339