Biopolymer from Adenanthera pavonina L. Seeds: Characterization, Photostability, Antioxidant Activity, and Biotoxicity Evaluation

Plant polysaccharides have been increasingly employed in the pharmaceutical, industrial, and food environments due to their versatile functional properties. In the present investigation, a heteropolysaccharide galactomannan (GAP) was extracted from Adenanthera pavonina L. seeds and characterized by...

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Main Authors: Roberta Cardoso Melo, Maurycyo Silva Geronço, Rayran Walter Ramos Sousa, Lara Polyana Silva Ramos, Francisca Pereira Araújo, Alessandra Braga Ribeiro, Paulo Michel Pinheiro Ferreira, Josy Anteveli Osajima, Marcília Pinheiro Costa
Format: Article
Language:English
Published: Wiley 2018-01-01
Series:International Journal of Polymer Science
Online Access:http://dx.doi.org/10.1155/2018/1385830
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author Roberta Cardoso Melo
Maurycyo Silva Geronço
Rayran Walter Ramos Sousa
Lara Polyana Silva Ramos
Francisca Pereira Araújo
Alessandra Braga Ribeiro
Paulo Michel Pinheiro Ferreira
Josy Anteveli Osajima
Marcília Pinheiro Costa
author_facet Roberta Cardoso Melo
Maurycyo Silva Geronço
Rayran Walter Ramos Sousa
Lara Polyana Silva Ramos
Francisca Pereira Araújo
Alessandra Braga Ribeiro
Paulo Michel Pinheiro Ferreira
Josy Anteveli Osajima
Marcília Pinheiro Costa
author_sort Roberta Cardoso Melo
collection DOAJ
description Plant polysaccharides have been increasingly employed in the pharmaceutical, industrial, and food environments due to their versatile functional properties. In the present investigation, a heteropolysaccharide galactomannan (GAP) was extracted from Adenanthera pavonina L. seeds and characterized by physicochemical analyses to determine its thermal properties, photostability, antioxidant activity, and acute toxicity. GAP was characterized by FTIR, DSC, and TG. The photostability of GAP submitted to artificial UV irradiation was analyzed. Antioxidant activity was evaluated by the DPPH (2,2-diphenyl-1-1-picrylhydrazyl) free radical-scavenging method, while a bioassay method was carried out to study acute toxicity in Artemia salina L. Physical-chemical and functional characteristics of GAP support its potential role in the food and pharmaceutical industries. GAP was photostable under UV irradiation. In vitro GAP antioxidant evaluation showed that it bears free radical-scavenging activity for DPPH radicals. The median lethal concentration (LC50) of GAP was 239.4 mg∙mL−1, indicating that this biopolymer is nontoxic. Such results indicate that this biopolymer presents characteristics of neutrality, photostability, and nontoxicity that are commercially attractive.
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spelling doaj-art-e1a30c9da43c4689b71f4b62297dbc202025-08-20T02:21:38ZengWileyInternational Journal of Polymer Science1687-94221687-94302018-01-01201810.1155/2018/13858301385830Biopolymer from Adenanthera pavonina L. Seeds: Characterization, Photostability, Antioxidant Activity, and Biotoxicity EvaluationRoberta Cardoso Melo0Maurycyo Silva Geronço1Rayran Walter Ramos Sousa2Lara Polyana Silva Ramos3Francisca Pereira Araújo4Alessandra Braga Ribeiro5Paulo Michel Pinheiro Ferreira6Josy Anteveli Osajima7Marcília Pinheiro Costa8Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, 64049-550 Teresina, PI, BrazilDepartment of Pharmacy, Federal University of Piauí, 64049-550 Teresina, PI, BrazilDepartment of Pharmacy, Federal University of Piauí, 64049-550 Teresina, PI, BrazilDepartment of Pharmacy, Federal University of Piauí, 64049-550 Teresina, PI, BrazilPostgraduate Program in Materials Science, Federal University of Piauí, 64049-550 Teresina, PI, BrazilDepartment of Pharmacy, Federal University of Piauí, 64049-550 Teresina, PI, BrazilPostgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, 64049-550 Teresina, PI, BrazilPostgraduate Program in Materials Science, Federal University of Piauí, 64049-550 Teresina, PI, BrazilPostgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, 64049-550 Teresina, PI, BrazilPlant polysaccharides have been increasingly employed in the pharmaceutical, industrial, and food environments due to their versatile functional properties. In the present investigation, a heteropolysaccharide galactomannan (GAP) was extracted from Adenanthera pavonina L. seeds and characterized by physicochemical analyses to determine its thermal properties, photostability, antioxidant activity, and acute toxicity. GAP was characterized by FTIR, DSC, and TG. The photostability of GAP submitted to artificial UV irradiation was analyzed. Antioxidant activity was evaluated by the DPPH (2,2-diphenyl-1-1-picrylhydrazyl) free radical-scavenging method, while a bioassay method was carried out to study acute toxicity in Artemia salina L. Physical-chemical and functional characteristics of GAP support its potential role in the food and pharmaceutical industries. GAP was photostable under UV irradiation. In vitro GAP antioxidant evaluation showed that it bears free radical-scavenging activity for DPPH radicals. The median lethal concentration (LC50) of GAP was 239.4 mg∙mL−1, indicating that this biopolymer is nontoxic. Such results indicate that this biopolymer presents characteristics of neutrality, photostability, and nontoxicity that are commercially attractive.http://dx.doi.org/10.1155/2018/1385830
spellingShingle Roberta Cardoso Melo
Maurycyo Silva Geronço
Rayran Walter Ramos Sousa
Lara Polyana Silva Ramos
Francisca Pereira Araújo
Alessandra Braga Ribeiro
Paulo Michel Pinheiro Ferreira
Josy Anteveli Osajima
Marcília Pinheiro Costa
Biopolymer from Adenanthera pavonina L. Seeds: Characterization, Photostability, Antioxidant Activity, and Biotoxicity Evaluation
International Journal of Polymer Science
title Biopolymer from Adenanthera pavonina L. Seeds: Characterization, Photostability, Antioxidant Activity, and Biotoxicity Evaluation
title_full Biopolymer from Adenanthera pavonina L. Seeds: Characterization, Photostability, Antioxidant Activity, and Biotoxicity Evaluation
title_fullStr Biopolymer from Adenanthera pavonina L. Seeds: Characterization, Photostability, Antioxidant Activity, and Biotoxicity Evaluation
title_full_unstemmed Biopolymer from Adenanthera pavonina L. Seeds: Characterization, Photostability, Antioxidant Activity, and Biotoxicity Evaluation
title_short Biopolymer from Adenanthera pavonina L. Seeds: Characterization, Photostability, Antioxidant Activity, and Biotoxicity Evaluation
title_sort biopolymer from adenanthera pavonina l seeds characterization photostability antioxidant activity and biotoxicity evaluation
url http://dx.doi.org/10.1155/2018/1385830
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