Simultaneous Determination of Dexamethasone, Ondansetron, Granisetron, Tropisetron, and Azasetron in Infusion Samples by HPLC with DAD Detection
A simple and rapid high-performance liquid chromatography with diode array detector (HPLC-DAD) method has been developed and validated for simultaneous quantification of five antiemetic agents in infusion samples: dexamethasone, ondansetron, granisetron, tropisetron, and azasetron. The chromatograph...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2017/6749087 |
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| author | Fu-chao Chen Lin-hai Wang Jun Guo Xiao-ya Shi Bao-xia Fang |
| author_facet | Fu-chao Chen Lin-hai Wang Jun Guo Xiao-ya Shi Bao-xia Fang |
| author_sort | Fu-chao Chen |
| collection | DOAJ |
| description | A simple and rapid high-performance liquid chromatography with diode array detector (HPLC-DAD) method has been developed and validated for simultaneous quantification of five antiemetic agents in infusion samples: dexamethasone, ondansetron, granisetron, tropisetron, and azasetron. The chromatographic separation was achieved on a Phenomenex C18 column (4.6 mm × 150 mm, 5 μm) using acetonitrile-50 mM KH2PO4 buffer-triethylamine (25 : 74 : 1; v/v; pH 4.0). Flow rate was 1.0 mL/min with a column temperature of 30°C. Validation of the method was made in terms of specificity, linearity, accuracy, and intra- and interday precision, as well as quantification and detection limits. The developed method can be used in the laboratory to routinely quantify dexamethasone, ondansetron, granisetron, tropisetron, and azasetron simultaneously and to evaluate the physicochemical stability of referred drugs in mixtures for endovenous use. |
| format | Article |
| id | doaj-art-e19c5c2c663b4323b9c358fd83d7f2b5 |
| institution | OA Journals |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-e19c5c2c663b4323b9c358fd83d7f2b52025-08-20T02:21:38ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732017-01-01201710.1155/2017/67490876749087Simultaneous Determination of Dexamethasone, Ondansetron, Granisetron, Tropisetron, and Azasetron in Infusion Samples by HPLC with DAD DetectionFu-chao Chen0Lin-hai Wang1Jun Guo2Xiao-ya Shi3Bao-xia Fang4Department of Pharmacy, Dongfeng Hospital, Hubei University of Medicine, Shiyan, Hubei 442008, ChinaDepartment of Pharmacy, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, ChinaDepartment of Oncology, Dongfeng Hospital, Hubei University of Medicine, Shiyan, Hubei 442008, ChinaDepartment of Pharmacy, Dongfeng Hospital, Hubei University of Medicine, Shiyan, Hubei 442008, ChinaDepartment of Pharmacy, Dongfeng Hospital, Hubei University of Medicine, Shiyan, Hubei 442008, ChinaA simple and rapid high-performance liquid chromatography with diode array detector (HPLC-DAD) method has been developed and validated for simultaneous quantification of five antiemetic agents in infusion samples: dexamethasone, ondansetron, granisetron, tropisetron, and azasetron. The chromatographic separation was achieved on a Phenomenex C18 column (4.6 mm × 150 mm, 5 μm) using acetonitrile-50 mM KH2PO4 buffer-triethylamine (25 : 74 : 1; v/v; pH 4.0). Flow rate was 1.0 mL/min with a column temperature of 30°C. Validation of the method was made in terms of specificity, linearity, accuracy, and intra- and interday precision, as well as quantification and detection limits. The developed method can be used in the laboratory to routinely quantify dexamethasone, ondansetron, granisetron, tropisetron, and azasetron simultaneously and to evaluate the physicochemical stability of referred drugs in mixtures for endovenous use.http://dx.doi.org/10.1155/2017/6749087 |
| spellingShingle | Fu-chao Chen Lin-hai Wang Jun Guo Xiao-ya Shi Bao-xia Fang Simultaneous Determination of Dexamethasone, Ondansetron, Granisetron, Tropisetron, and Azasetron in Infusion Samples by HPLC with DAD Detection Journal of Analytical Methods in Chemistry |
| title | Simultaneous Determination of Dexamethasone, Ondansetron, Granisetron, Tropisetron, and Azasetron in Infusion Samples by HPLC with DAD Detection |
| title_full | Simultaneous Determination of Dexamethasone, Ondansetron, Granisetron, Tropisetron, and Azasetron in Infusion Samples by HPLC with DAD Detection |
| title_fullStr | Simultaneous Determination of Dexamethasone, Ondansetron, Granisetron, Tropisetron, and Azasetron in Infusion Samples by HPLC with DAD Detection |
| title_full_unstemmed | Simultaneous Determination of Dexamethasone, Ondansetron, Granisetron, Tropisetron, and Azasetron in Infusion Samples by HPLC with DAD Detection |
| title_short | Simultaneous Determination of Dexamethasone, Ondansetron, Granisetron, Tropisetron, and Azasetron in Infusion Samples by HPLC with DAD Detection |
| title_sort | simultaneous determination of dexamethasone ondansetron granisetron tropisetron and azasetron in infusion samples by hplc with dad detection |
| url | http://dx.doi.org/10.1155/2017/6749087 |
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