Extracellular electron transfer based methylotrophic methanogenesis in paddy soil and the prevalent Methanomassiliicoccus
Abstract The biogenic methane contributes obviously to global warming, and direct interspecies electron transfer (DIET) driven CO2 reductive methanogenesis is one of the important methanogenic pathways. Here we report DIET-driven methylotrophic methanogenesis in paddy soil and the prevalent Methanom...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-04-01
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| Series: | Communications Earth & Environment |
| Online Access: | https://doi.org/10.1038/s43247-025-02276-3 |
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| author | Lingyan Li Xuping Tian Xuemeng Wang Chuan Chen Qi Zhou Lei Qi Jie Li Kai Xue Fangjie Zhao Yanfen Wang Xiuzhu Dong |
| author_facet | Lingyan Li Xuping Tian Xuemeng Wang Chuan Chen Qi Zhou Lei Qi Jie Li Kai Xue Fangjie Zhao Yanfen Wang Xiuzhu Dong |
| author_sort | Lingyan Li |
| collection | DOAJ |
| description | Abstract The biogenic methane contributes obviously to global warming, and direct interspecies electron transfer (DIET) driven CO2 reductive methanogenesis is one of the important methanogenic pathways. Here we report DIET-driven methylotrophic methanogenesis in paddy soil and the prevalent Methanomassiliicoccus. M. luminyensis CZDD1 exhibited a 1.9-fold higher methanol-derived methanogenic rate when cocultured with Clostridium malenominatum than its H2-dependent monoculture. Coculturing with Geobacter metallireducens, a known extracellular electron producer, CZDD1 showed the same efficient methanol-derived methane production, thus identified DIET-based methylotrophic methanogenesis. Chronoamperometry detected efficient methane production by M. luminyensis CZDD1 and two paddy soils from methanol and dimethylarsenate in accompany with current consumption. Differential transcriptomics predicted a membrane-bound Fpo-like complex of CZDD1 for uptake of extracellular electrons. Co-occurrence of Methanomassiliicoccaceae with Geobacteraceae and Clostridiaceae was found in five Chinese paddy soils, and Methanomassiliicoccus is ubiquitously distributed among various anoxic environments. Therefore, DIET-driven methylotrophic methanogenesis can be an important mechanism in methane emission. |
| format | Article |
| id | doaj-art-e1965c6668744e78856f33b7654c6415 |
| institution | OA Journals |
| issn | 2662-4435 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
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| series | Communications Earth & Environment |
| spelling | doaj-art-e1965c6668744e78856f33b7654c64152025-08-20T02:27:11ZengNature PortfolioCommunications Earth & Environment2662-44352025-04-016111110.1038/s43247-025-02276-3Extracellular electron transfer based methylotrophic methanogenesis in paddy soil and the prevalent MethanomassiliicoccusLingyan Li0Xuping Tian1Xuemeng Wang2Chuan Chen3Qi Zhou4Lei Qi5Jie Li6Kai Xue7Fangjie Zhao8Yanfen Wang9Xiuzhu Dong10State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of SciencesCollege of Resources and Environment, University of Chinese Academy of SciencesState Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Jiangsu Provincial Key Laboratory for Organic Solid Waste Utilization, College of Resources and Environmental Sciences, Nanjing Agricultural UniversityState Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of SciencesState Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of SciencesCollege of Resources and Environment, University of Chinese Academy of SciencesState Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Jiangsu Provincial Key Laboratory for Organic Solid Waste Utilization, College of Resources and Environmental Sciences, Nanjing Agricultural UniversityCollege of Resources and Environment, University of Chinese Academy of SciencesState Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of SciencesAbstract The biogenic methane contributes obviously to global warming, and direct interspecies electron transfer (DIET) driven CO2 reductive methanogenesis is one of the important methanogenic pathways. Here we report DIET-driven methylotrophic methanogenesis in paddy soil and the prevalent Methanomassiliicoccus. M. luminyensis CZDD1 exhibited a 1.9-fold higher methanol-derived methanogenic rate when cocultured with Clostridium malenominatum than its H2-dependent monoculture. Coculturing with Geobacter metallireducens, a known extracellular electron producer, CZDD1 showed the same efficient methanol-derived methane production, thus identified DIET-based methylotrophic methanogenesis. Chronoamperometry detected efficient methane production by M. luminyensis CZDD1 and two paddy soils from methanol and dimethylarsenate in accompany with current consumption. Differential transcriptomics predicted a membrane-bound Fpo-like complex of CZDD1 for uptake of extracellular electrons. Co-occurrence of Methanomassiliicoccaceae with Geobacteraceae and Clostridiaceae was found in five Chinese paddy soils, and Methanomassiliicoccus is ubiquitously distributed among various anoxic environments. Therefore, DIET-driven methylotrophic methanogenesis can be an important mechanism in methane emission.https://doi.org/10.1038/s43247-025-02276-3 |
| spellingShingle | Lingyan Li Xuping Tian Xuemeng Wang Chuan Chen Qi Zhou Lei Qi Jie Li Kai Xue Fangjie Zhao Yanfen Wang Xiuzhu Dong Extracellular electron transfer based methylotrophic methanogenesis in paddy soil and the prevalent Methanomassiliicoccus Communications Earth & Environment |
| title | Extracellular electron transfer based methylotrophic methanogenesis in paddy soil and the prevalent Methanomassiliicoccus |
| title_full | Extracellular electron transfer based methylotrophic methanogenesis in paddy soil and the prevalent Methanomassiliicoccus |
| title_fullStr | Extracellular electron transfer based methylotrophic methanogenesis in paddy soil and the prevalent Methanomassiliicoccus |
| title_full_unstemmed | Extracellular electron transfer based methylotrophic methanogenesis in paddy soil and the prevalent Methanomassiliicoccus |
| title_short | Extracellular electron transfer based methylotrophic methanogenesis in paddy soil and the prevalent Methanomassiliicoccus |
| title_sort | extracellular electron transfer based methylotrophic methanogenesis in paddy soil and the prevalent methanomassiliicoccus |
| url | https://doi.org/10.1038/s43247-025-02276-3 |
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