HEG1 as a novel potential biomarker for the prognosis of lung adenocarcinoma
Abstract Background Heart development protein with EGF‐like domains 1 (HEG1), generally related to angiogenesis and embryonic development, was reported to participate in the occurrence and progression of some tumors recently. However, the role of HEG1 in lung adenocarcinoma (LUAD) is unclear. Patien...
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| Format: | Article |
| Language: | English |
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Wiley
2023-02-01
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| Series: | Cancer Medicine |
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| Online Access: | https://doi.org/10.1002/cam4.5081 |
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| author | Xin Zou Yue Zhang Ning Wang Jie Shi Qinghai Li Wanming Hao Wenjing Zhu Wei Han |
| author_facet | Xin Zou Yue Zhang Ning Wang Jie Shi Qinghai Li Wanming Hao Wenjing Zhu Wei Han |
| author_sort | Xin Zou |
| collection | DOAJ |
| description | Abstract Background Heart development protein with EGF‐like domains 1 (HEG1), generally related to angiogenesis and embryonic development, was reported to participate in the occurrence and progression of some tumors recently. However, the role of HEG1 in lung adenocarcinoma (LUAD) is unclear. Patients and Methods To explore the effect of HEG1 on LUAD, GEPIA platform and UALCAN database, as well as Kaplan–Meier plotter were adopted to analyze the association of HEG1 with clinicopathological characteristics and survival outcomes for LUAD firstly. And then the HEG1 in LUAD tissues, blood and cell lines were detected by qRT‐PCR, western blot, immunofluorescence, immunohistochemistry, and ELISA. Gene set enrichment analysis (GSEA) was conducted to identify pathways that might be affected by HEG1 in LUAD. Results In this study, HEG1 in lung tissues and cell lines of LUAD were significantly downregulated compared to benign pulmonary disease tissues and alveolar epithelial cells (p < 0.05). Moreover, compared with other groups, patients with advanced tumor stage had lower HEG1 mRNA expression levels (p = 0.025), which were negatively correlated with Ki67 index in tumor tissues (r = −0.427, p = 0.033). On the other hand, the LUAD patients with lower HEG1 had shorter overall survival (OS) (HR = 0.51, 95% CI: 0.40–0.65, p < 0.001) according to Kaplan–Meier plotter. In addition, HEG1 in serum of LUAD patients was negatively associated with CEA (r = −0.636, p < 0.001). GSEA showed that HEG1 was enriched in various metabolic‐related pathways, including glucose metabolism, lipid metabolism, and nucleotide metabolism signaling. Conclusions HEG1 was downregulated in LUAD patients and associated with poor prognosis, which indicating HEG1 may serve as a potential biomarker for diagnosis and prognosis of LUAD. |
| format | Article |
| id | doaj-art-e195e6ef28fe42c9abec581e41d96d13 |
| institution | OA Journals |
| issn | 2045-7634 |
| language | English |
| publishDate | 2023-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj-art-e195e6ef28fe42c9abec581e41d96d132025-08-20T02:23:32ZengWileyCancer Medicine2045-76342023-02-011233288329810.1002/cam4.5081HEG1 as a novel potential biomarker for the prognosis of lung adenocarcinomaXin Zou0Yue Zhang1Ning Wang2Jie Shi3Qinghai Li4Wanming Hao5Wenjing Zhu6Wei Han7Department of Pathology, Qingdao Municipal Hospital Dalian Medical University Qingdao ChinaDepartment of Respiratory Jilin Provincial People's Hospital Jilin ChinaDepartment of Pulmonary and Critical Care Medicine, Qingdao Municipal Hospital, School of Medicine Qingdao University Qingdao ChinaNMPA Key Laboratory for Quality Research and Evaluation of Traditional Marine Chinese Medicine Qingdao ChinaRespiratory Disease Key Laboratory of Qingdao Qingdao Municipal Hospital Qingdao ChinaDepartment of Pulmonary and Critical Care Medicine, Qingdao Municipal Hospital, School of Medicine Qingdao University Qingdao ChinaRespiratory Disease Key Laboratory of Qingdao Qingdao Municipal Hospital Qingdao ChinaRespiratory Disease Key Laboratory of Qingdao Qingdao Municipal Hospital Qingdao ChinaAbstract Background Heart development protein with EGF‐like domains 1 (HEG1), generally related to angiogenesis and embryonic development, was reported to participate in the occurrence and progression of some tumors recently. However, the role of HEG1 in lung adenocarcinoma (LUAD) is unclear. Patients and Methods To explore the effect of HEG1 on LUAD, GEPIA platform and UALCAN database, as well as Kaplan–Meier plotter were adopted to analyze the association of HEG1 with clinicopathological characteristics and survival outcomes for LUAD firstly. And then the HEG1 in LUAD tissues, blood and cell lines were detected by qRT‐PCR, western blot, immunofluorescence, immunohistochemistry, and ELISA. Gene set enrichment analysis (GSEA) was conducted to identify pathways that might be affected by HEG1 in LUAD. Results In this study, HEG1 in lung tissues and cell lines of LUAD were significantly downregulated compared to benign pulmonary disease tissues and alveolar epithelial cells (p < 0.05). Moreover, compared with other groups, patients with advanced tumor stage had lower HEG1 mRNA expression levels (p = 0.025), which were negatively correlated with Ki67 index in tumor tissues (r = −0.427, p = 0.033). On the other hand, the LUAD patients with lower HEG1 had shorter overall survival (OS) (HR = 0.51, 95% CI: 0.40–0.65, p < 0.001) according to Kaplan–Meier plotter. In addition, HEG1 in serum of LUAD patients was negatively associated with CEA (r = −0.636, p < 0.001). GSEA showed that HEG1 was enriched in various metabolic‐related pathways, including glucose metabolism, lipid metabolism, and nucleotide metabolism signaling. Conclusions HEG1 was downregulated in LUAD patients and associated with poor prognosis, which indicating HEG1 may serve as a potential biomarker for diagnosis and prognosis of LUAD.https://doi.org/10.1002/cam4.5081biomarkerHEG1lung adenocarcinomaprognosis |
| spellingShingle | Xin Zou Yue Zhang Ning Wang Jie Shi Qinghai Li Wanming Hao Wenjing Zhu Wei Han HEG1 as a novel potential biomarker for the prognosis of lung adenocarcinoma Cancer Medicine biomarker HEG1 lung adenocarcinoma prognosis |
| title | HEG1 as a novel potential biomarker for the prognosis of lung adenocarcinoma |
| title_full | HEG1 as a novel potential biomarker for the prognosis of lung adenocarcinoma |
| title_fullStr | HEG1 as a novel potential biomarker for the prognosis of lung adenocarcinoma |
| title_full_unstemmed | HEG1 as a novel potential biomarker for the prognosis of lung adenocarcinoma |
| title_short | HEG1 as a novel potential biomarker for the prognosis of lung adenocarcinoma |
| title_sort | heg1 as a novel potential biomarker for the prognosis of lung adenocarcinoma |
| topic | biomarker HEG1 lung adenocarcinoma prognosis |
| url | https://doi.org/10.1002/cam4.5081 |
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