Diagnostic In Vivo Sensing of COVID-19 Antibody Detection Using DNA-Linking Graphene Oxide Synthetic Mimic Skin Tattoo Probes
COVID-19 antibody detection is dependent on highly specialized, time-consuming techniques, such as PCR separation, DNA amplification, and other methods such as spectrophotometric absorption. For these reasons, specialized technical training is necessary because individual diagnostic treatment is dif...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/13/2/354 |
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| author | Kyung Lee Dong Ho Kim Sihyun Jun Yeseul Oh Ye Jun Oh Seo Jun Lee Keumsook Kim Suw Young Ly |
| author_facet | Kyung Lee Dong Ho Kim Sihyun Jun Yeseul Oh Ye Jun Oh Seo Jun Lee Keumsook Kim Suw Young Ly |
| author_sort | Kyung Lee |
| collection | DOAJ |
| description | COVID-19 antibody detection is dependent on highly specialized, time-consuming techniques, such as PCR separation, DNA amplification, and other methods such as spectrophotometric absorption. For these reasons, specialized technical training is necessary because individual diagnostic treatment is difficult. We have attempted to perform rapid sensing with a detection time of only 30 s. Additionally, we used a wearable multi-layer graphene oxide nanocolloid synthetic skin tattoo probe assay for influenza and COVID-19 virus detection with an electrochemical antigen–antibody redox ionic titration circuit. Cyclic voltametric−2 V~2.0 V potential windows were used. The diagnostic detection limit was determined using stripping anodic and cathodic amplifiers, and the working probe was fabricated with a graphene molecule structure with a virus antigen-immobilized amplifier. With redox potential strength obtained within −1.0 V~−1.3 V ionic activity, anodic and cathodic current linearly increased in the phosphate-buffered saline 5 mL electrolyte. The results indicate that instant detection was enabled via individual and wearable tattoo sensors. |
| format | Article |
| id | doaj-art-e195d7a09380455fb5ebfbce116072db |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-e195d7a09380455fb5ebfbce116072db2025-08-20T02:45:00ZengMDPI AGMicroorganisms2076-26072025-02-0113235410.3390/microorganisms13020354Diagnostic In Vivo Sensing of COVID-19 Antibody Detection Using DNA-Linking Graphene Oxide Synthetic Mimic Skin Tattoo ProbesKyung Lee0Dong Ho Kim1Sihyun Jun2Yeseul Oh3Ye Jun Oh4Seo Jun Lee5Keumsook Kim6Suw Young Ly7Biosensor Research Institute, Seoul National University of Science & Technology, Seoul 01811, Republic of KoreaUniversity of Michigan College of Pharmacy, 428 Church St, Ann Arbor, MI 48109, USADepartment of Radiological & Clinical Research, Korea Cancer Center Hospital, Korea Institute of Radiological and Medical Sciences, Seoul 01812, Republic of KoreaBiosensor Research Institute, Seoul National University of Science & Technology, Seoul 01811, Republic of KoreaBiosensor Research Institute, Seoul National University of Science & Technology, Seoul 01811, Republic of KoreaBiosensor Research Institute, Seoul National University of Science & Technology, Seoul 01811, Republic of KoreaBiosensor Research Institute, Seoul National University of Science & Technology, Seoul 01811, Republic of KoreaBiosensor Research Institute, Seoul National University of Science & Technology, Seoul 01811, Republic of KoreaCOVID-19 antibody detection is dependent on highly specialized, time-consuming techniques, such as PCR separation, DNA amplification, and other methods such as spectrophotometric absorption. For these reasons, specialized technical training is necessary because individual diagnostic treatment is difficult. We have attempted to perform rapid sensing with a detection time of only 30 s. Additionally, we used a wearable multi-layer graphene oxide nanocolloid synthetic skin tattoo probe assay for influenza and COVID-19 virus detection with an electrochemical antigen–antibody redox ionic titration circuit. Cyclic voltametric−2 V~2.0 V potential windows were used. The diagnostic detection limit was determined using stripping anodic and cathodic amplifiers, and the working probe was fabricated with a graphene molecule structure with a virus antigen-immobilized amplifier. With redox potential strength obtained within −1.0 V~−1.3 V ionic activity, anodic and cathodic current linearly increased in the phosphate-buffered saline 5 mL electrolyte. The results indicate that instant detection was enabled via individual and wearable tattoo sensors.https://www.mdpi.com/2076-2607/13/2/354grapheneDNA immobilizedinfluenzaCOVID-19viruselectrochemical |
| spellingShingle | Kyung Lee Dong Ho Kim Sihyun Jun Yeseul Oh Ye Jun Oh Seo Jun Lee Keumsook Kim Suw Young Ly Diagnostic In Vivo Sensing of COVID-19 Antibody Detection Using DNA-Linking Graphene Oxide Synthetic Mimic Skin Tattoo Probes Microorganisms graphene DNA immobilized influenza COVID-19 virus electrochemical |
| title | Diagnostic In Vivo Sensing of COVID-19 Antibody Detection Using DNA-Linking Graphene Oxide Synthetic Mimic Skin Tattoo Probes |
| title_full | Diagnostic In Vivo Sensing of COVID-19 Antibody Detection Using DNA-Linking Graphene Oxide Synthetic Mimic Skin Tattoo Probes |
| title_fullStr | Diagnostic In Vivo Sensing of COVID-19 Antibody Detection Using DNA-Linking Graphene Oxide Synthetic Mimic Skin Tattoo Probes |
| title_full_unstemmed | Diagnostic In Vivo Sensing of COVID-19 Antibody Detection Using DNA-Linking Graphene Oxide Synthetic Mimic Skin Tattoo Probes |
| title_short | Diagnostic In Vivo Sensing of COVID-19 Antibody Detection Using DNA-Linking Graphene Oxide Synthetic Mimic Skin Tattoo Probes |
| title_sort | diagnostic in vivo sensing of covid 19 antibody detection using dna linking graphene oxide synthetic mimic skin tattoo probes |
| topic | graphene DNA immobilized influenza COVID-19 virus electrochemical |
| url | https://www.mdpi.com/2076-2607/13/2/354 |
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