Activation studies with amino acids and amines of a β-carbonic anhydrase from Mammaliicoccus (Staphylococcus) sciuri previously annotated as Staphylococcus aureus (SauBCA) carbonic anhydrase

A β-carbonic anhydrase (CA, EC 4.2.1.1) previously annotated to be present in the genome of Staphylococcus aureus, SauBCA, has been shown to belong to another pathogenic bacterium, Mammaliicoccus (Staphylococcus) sciuri. This enzyme, MscCA, has been investigated for its activation with a series of n...

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Main Authors: Andrea Angeli, Linda J Urbański, Clemente Capasso, Seppo Parkkila, Claudiu T. Supuran
Format: Article
Language:English
Published: Taylor & Francis Group 2022-12-01
Series:Journal of Enzyme Inhibition and Medicinal Chemistry
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Online Access:https://www.tandfonline.com/doi/10.1080/14756366.2022.2131780
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author Andrea Angeli
Linda J Urbański
Clemente Capasso
Seppo Parkkila
Claudiu T. Supuran
author_facet Andrea Angeli
Linda J Urbański
Clemente Capasso
Seppo Parkkila
Claudiu T. Supuran
author_sort Andrea Angeli
collection DOAJ
description A β-carbonic anhydrase (CA, EC 4.2.1.1) previously annotated to be present in the genome of Staphylococcus aureus, SauBCA, has been shown to belong to another pathogenic bacterium, Mammaliicoccus (Staphylococcus) sciuri. This enzyme, MscCA, has been investigated for its activation with a series of natural and synthetic amino acid and amines, comparing the results with those obtained for the ortholog enzyme from Escherichia coli, EcoCAβ. The best MscCA activators were D-His, L- and D-DOPA, 4-(2-aminoethyl)-morpholine and L-Asn, which showed KAs of 0.12 − 0.89 µM. The least efficient activators were D-Tyr and L-Gln (KAs of 13.9 − 28.6 µM). The enzyme was also also inhibited by anions and sulphonamides, as described earlier. Endogenous CA activators may play a role in bacterial virulence and colonisation of the host which makes this research topic of great interest.
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publishDate 2022-12-01
publisher Taylor & Francis Group
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series Journal of Enzyme Inhibition and Medicinal Chemistry
spelling doaj-art-e18514fffad44559bc26538e46288bea2025-08-20T02:36:30ZengTaylor & Francis GroupJournal of Enzyme Inhibition and Medicinal Chemistry1475-63661475-63742022-12-013712786279210.1080/14756366.2022.2131780Activation studies with amino acids and amines of a β-carbonic anhydrase from Mammaliicoccus (Staphylococcus) sciuri previously annotated as Staphylococcus aureus (SauBCA) carbonic anhydraseAndrea Angeli0Linda J Urbański1Clemente Capasso2Seppo Parkkila3Claudiu T. Supuran4Dipartimento Neurofarba, Sezione di Scienze Farmaceutiche e Nutraceutiche, Università degli Studi di Firenze, Sesto Fiorentino (Florence), ItalyFaculty of Medicine and Health Technology, Tampere University, Tampere, FinlandDepartment of Biology, Agriculture and Food Sciences, Institute of Biosciences and Bioresources, Napoli, ItalyFaculty of Medicine and Health Technology, Tampere University, Tampere, FinlandDipartimento Neurofarba, Sezione di Scienze Farmaceutiche e Nutraceutiche, Università degli Studi di Firenze, Sesto Fiorentino (Florence), ItalyA β-carbonic anhydrase (CA, EC 4.2.1.1) previously annotated to be present in the genome of Staphylococcus aureus, SauBCA, has been shown to belong to another pathogenic bacterium, Mammaliicoccus (Staphylococcus) sciuri. This enzyme, MscCA, has been investigated for its activation with a series of natural and synthetic amino acid and amines, comparing the results with those obtained for the ortholog enzyme from Escherichia coli, EcoCAβ. The best MscCA activators were D-His, L- and D-DOPA, 4-(2-aminoethyl)-morpholine and L-Asn, which showed KAs of 0.12 − 0.89 µM. The least efficient activators were D-Tyr and L-Gln (KAs of 13.9 − 28.6 µM). The enzyme was also also inhibited by anions and sulphonamides, as described earlier. Endogenous CA activators may play a role in bacterial virulence and colonisation of the host which makes this research topic of great interest.https://www.tandfonline.com/doi/10.1080/14756366.2022.2131780Staphylococcaceaecarbonic anhydraseactivatoramine/amino acidMammaliicoccus (Staphylococcus) sciuri
spellingShingle Andrea Angeli
Linda J Urbański
Clemente Capasso
Seppo Parkkila
Claudiu T. Supuran
Activation studies with amino acids and amines of a β-carbonic anhydrase from Mammaliicoccus (Staphylococcus) sciuri previously annotated as Staphylococcus aureus (SauBCA) carbonic anhydrase
Journal of Enzyme Inhibition and Medicinal Chemistry
Staphylococcaceae
carbonic anhydrase
activator
amine/amino acid
Mammaliicoccus (Staphylococcus) sciuri
title Activation studies with amino acids and amines of a β-carbonic anhydrase from Mammaliicoccus (Staphylococcus) sciuri previously annotated as Staphylococcus aureus (SauBCA) carbonic anhydrase
title_full Activation studies with amino acids and amines of a β-carbonic anhydrase from Mammaliicoccus (Staphylococcus) sciuri previously annotated as Staphylococcus aureus (SauBCA) carbonic anhydrase
title_fullStr Activation studies with amino acids and amines of a β-carbonic anhydrase from Mammaliicoccus (Staphylococcus) sciuri previously annotated as Staphylococcus aureus (SauBCA) carbonic anhydrase
title_full_unstemmed Activation studies with amino acids and amines of a β-carbonic anhydrase from Mammaliicoccus (Staphylococcus) sciuri previously annotated as Staphylococcus aureus (SauBCA) carbonic anhydrase
title_short Activation studies with amino acids and amines of a β-carbonic anhydrase from Mammaliicoccus (Staphylococcus) sciuri previously annotated as Staphylococcus aureus (SauBCA) carbonic anhydrase
title_sort activation studies with amino acids and amines of a β carbonic anhydrase from mammaliicoccus staphylococcus sciuri previously annotated as staphylococcus aureus saubca carbonic anhydrase
topic Staphylococcaceae
carbonic anhydrase
activator
amine/amino acid
Mammaliicoccus (Staphylococcus) sciuri
url https://www.tandfonline.com/doi/10.1080/14756366.2022.2131780
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