Distribution of Bacillus cereus and vomiting toxin gene in cooked rice samples which simulated contaminated by raw vegetables
ObjectiveTo analyze the distribution of Bacillus cereus （B. cereus） and vomiting toxin gene （ces） in cooked rice samples which simulated contaminated by raw vegetables， and provide basic data for prevention of food poisoning caused by B.cereus.MethodsFifty raw vegetable samples were collected in thi...
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The Editorial Office of Chinese Journal of Food Hygiene
2024-04-01
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| Series: | Zhongguo shipin weisheng zazhi |
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| Online Access: | https://www.zgspws.com/zgspwszz/article/abstract/202404012?st=article_issue |
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| author | LI Ying QUAN Fei CUI Juanjuan WANG Yuanyuan WANG Miao JING Hongbo LIU Shengtian PENG Tao PANG Bo |
| author_facet | LI Ying QUAN Fei CUI Juanjuan WANG Yuanyuan WANG Miao JING Hongbo LIU Shengtian PENG Tao PANG Bo |
| author_sort | LI Ying |
| collection | DOAJ |
| description | ObjectiveTo analyze the distribution of Bacillus cereus （B. cereus） and vomiting toxin gene （ces） in cooked rice samples which simulated contaminated by raw vegetables， and provide basic data for prevention of food poisoning caused by B.cereus.MethodsFifty raw vegetable samples were collected in this study. Simulate method was as contaminated “freshly cooked rice” with 0.85% physiological saline solution from washing raw vegetables， and then place it in a 30 ℃ 70% RH environment for 24 h to prepare a sample of “contaminated rice”. Plate counting， real time PCR and digital PCR were performed for raw vegetable and “contaminated rice” samples. Statistical analysis was performed on the detection rates grouped by different vegetable types for raw vegetable samples and “contaminated rice” contaminated with them.ResultsThe detection ratios of B.cereus， ces gene and Bac16s RNA gene in raw vegetables was 80.00% （40/50）， 0（0/50） and 10.00%（5/50）. The detection ratios of B.cereus， ces gene and Bac16s RNA gene in “contaminated rice” was 94.00% （47/50）， 14.00% （7/50） and 90.00% （45/50）. There was significant difference in the detection ratio of B.cereus between raw vegetables collected from agricultural markets and farmland（χ2=11.063， P=0.000 88 correction）， and the difference in the detection rates of Bac16s RNA gene and ces genes in “contaminated rice” contaminated with the two groups of raw vegetables mentioned above is statistically significant（χ2=3.926， P=0.047 5 correction； χ2=5.444， P=0.019 6 correction）. Seven “contaminated rice” were tested positive for the ces gene using real time PCR， with Ct values ranging from 24.12 to 37.73， and digital PCR results ranging from 6.8 copes/μL-6.2×106 copes/μL.ConclusionCooked rice samples which simulated contaminated by raw vegetables might be has the etiology characteristics that lead to a risk of food poisoning caused by B.cereus. |
| format | Article |
| id | doaj-art-e180ca16935f45e1a3074fe58f00007c |
| institution | OA Journals |
| issn | 1004-8456 |
| language | zho |
| publishDate | 2024-04-01 |
| publisher | The Editorial Office of Chinese Journal of Food Hygiene |
| record_format | Article |
| series | Zhongguo shipin weisheng zazhi |
| spelling | doaj-art-e180ca16935f45e1a3074fe58f00007c2025-08-20T02:11:29ZzhoThe Editorial Office of Chinese Journal of Food HygieneZhongguo shipin weisheng zazhi1004-84562024-04-0136444044410.13590/j.cjfh.2024.04.0121004-8456（2024）04-0440-05Distribution of Bacillus cereus and vomiting toxin gene in cooked rice samples which simulated contaminated by raw vegetablesLI Ying0QUAN Fei1CUI Juanjuan2WANG Yuanyuan3WANG Miao4JING Hongbo5LIU Shengtian6PENG Tao7PANG Bo8Shunyi District Center for Disease Control and Prevention, Beijing 101300, ChinaShunyi District Center for Disease Control and Prevention, Beijing 101300, ChinaBaotou Medical College, Inner Mongolia Baotou 010040, ChinaShunyi District Center for Disease Control and Prevention, Beijing 101300, ChinaShunyi District Center for Disease Control and Prevention, Beijing 101300, ChinaShunyi District Center for Disease Control and Prevention, Beijing 101300, ChinaShunyi District Center for Disease Control and Prevention, Beijing 101300, ChinaShunyi District Center for Disease Control and Prevention, Beijing 101300, ChinaWorkstation for Microbial Infectious Disease, Beijing 101300, ChinaObjectiveTo analyze the distribution of Bacillus cereus （B. cereus） and vomiting toxin gene （ces） in cooked rice samples which simulated contaminated by raw vegetables， and provide basic data for prevention of food poisoning caused by B.cereus.MethodsFifty raw vegetable samples were collected in this study. Simulate method was as contaminated “freshly cooked rice” with 0.85% physiological saline solution from washing raw vegetables， and then place it in a 30 ℃ 70% RH environment for 24 h to prepare a sample of “contaminated rice”. Plate counting， real time PCR and digital PCR were performed for raw vegetable and “contaminated rice” samples. Statistical analysis was performed on the detection rates grouped by different vegetable types for raw vegetable samples and “contaminated rice” contaminated with them.ResultsThe detection ratios of B.cereus， ces gene and Bac16s RNA gene in raw vegetables was 80.00% （40/50）， 0（0/50） and 10.00%（5/50）. The detection ratios of B.cereus， ces gene and Bac16s RNA gene in “contaminated rice” was 94.00% （47/50）， 14.00% （7/50） and 90.00% （45/50）. There was significant difference in the detection ratio of B.cereus between raw vegetables collected from agricultural markets and farmland（χ2=11.063， P=0.000 88 correction）， and the difference in the detection rates of Bac16s RNA gene and ces genes in “contaminated rice” contaminated with the two groups of raw vegetables mentioned above is statistically significant（χ2=3.926， P=0.047 5 correction； χ2=5.444， P=0.019 6 correction）. Seven “contaminated rice” were tested positive for the ces gene using real time PCR， with Ct values ranging from 24.12 to 37.73， and digital PCR results ranging from 6.8 copes/μL-6.2×106 copes/μL.ConclusionCooked rice samples which simulated contaminated by raw vegetables might be has the etiology characteristics that lead to a risk of food poisoning caused by B.cereus.https://www.zgspws.com/zgspwszz/article/abstract/202404012?st=article_issuebacillus cereusriceraw vegetablesvomiting toxin |
| spellingShingle | LI Ying QUAN Fei CUI Juanjuan WANG Yuanyuan WANG Miao JING Hongbo LIU Shengtian PENG Tao PANG Bo Distribution of Bacillus cereus and vomiting toxin gene in cooked rice samples which simulated contaminated by raw vegetables Zhongguo shipin weisheng zazhi bacillus cereus rice raw vegetables vomiting toxin |
| title | Distribution of Bacillus cereus and vomiting toxin gene in cooked rice samples which simulated contaminated by raw vegetables |
| title_full | Distribution of Bacillus cereus and vomiting toxin gene in cooked rice samples which simulated contaminated by raw vegetables |
| title_fullStr | Distribution of Bacillus cereus and vomiting toxin gene in cooked rice samples which simulated contaminated by raw vegetables |
| title_full_unstemmed | Distribution of Bacillus cereus and vomiting toxin gene in cooked rice samples which simulated contaminated by raw vegetables |
| title_short | Distribution of Bacillus cereus and vomiting toxin gene in cooked rice samples which simulated contaminated by raw vegetables |
| title_sort | distribution of bacillus cereus and vomiting toxin gene in cooked rice samples which simulated contaminated by raw vegetables |
| topic | bacillus cereus rice raw vegetables vomiting toxin |
| url | https://www.zgspws.com/zgspwszz/article/abstract/202404012?st=article_issue |
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