Intraventricular continuous BDNF administration ameliorates neuroinflammation and enhances neurogenesis against rodent intracerebral hemorrhage model
ObjectiveBrain-derived neurotrophic factor (BDNF) is a pivotal growth factor for neuronal survival; however, its precise role following intracerebral hemorrhage (ICH) remains poorly understood. This study sought to investigate whether intraventricular administration of BDNF could enhance neurogenesi...
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Frontiers Media S.A.
2025-08-01
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| Series: | Frontiers in Neurology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fneur.2025.1606606/full |
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| author | Ting-Chun Lin Masahito Kawabori Erika Yoshie Yo Nakahara Li-Kai Tsai Miki Fujimura |
| author_facet | Ting-Chun Lin Masahito Kawabori Erika Yoshie Yo Nakahara Li-Kai Tsai Miki Fujimura |
| author_sort | Ting-Chun Lin |
| collection | DOAJ |
| description | ObjectiveBrain-derived neurotrophic factor (BDNF) is a pivotal growth factor for neuronal survival; however, its precise role following intracerebral hemorrhage (ICH) remains poorly understood. This study sought to investigate whether intraventricular administration of BDNF could enhance neurogenesis and ameliorate neuroinflammation, resulting in improvement of neurological outcomes in a rat ICH model.MethodsMale Sprague–Dawley rats were utilized to create an acute ICH model by collagenase infusion into the internal capsule. An intraventricular minipump was subcutaneously implanted, with the catheter tip inserted into the contralateral ventricle to deliver BDNF for 7 consecutive days. The rats were assigned to one of three experimental groups; the BDNF group, the anti-BDNF antibody group, and the sham group. Functional assessment was conducted up to 14 days post-ICH, and immunohistochemical analysis was performed to evaluate neurogenesis, apoptosis, and neuroinflammation in the perihematomal area and the subventricular zone (SVZ).ResultsBrain-derived neurotrophic factor treatment significantly increased the proliferation of neural stem cells in the perihematomal region. It also reduced the neuroinflammation 14 days post-ICH. Additionally, BDNF treatment demonstrated a favorable neurological function at 14 days post-ICH.ConclusionIntraventricular administration of BDNF demonstrated favorable recovery after ICH by reducing inflammation and enhancing neurogenesis. |
| format | Article |
| id | doaj-art-e17c18e206ce4736b3dce357aacb80d8 |
| institution | Kabale University |
| issn | 1664-2295 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Neurology |
| spelling | doaj-art-e17c18e206ce4736b3dce357aacb80d82025-08-20T03:41:41ZengFrontiers Media S.A.Frontiers in Neurology1664-22952025-08-011610.3389/fneur.2025.16066061606606Intraventricular continuous BDNF administration ameliorates neuroinflammation and enhances neurogenesis against rodent intracerebral hemorrhage modelTing-Chun Lin0Masahito Kawabori1Erika Yoshie2Yo Nakahara3Li-Kai Tsai4Miki Fujimura5Department of Neurosurgery, Hokkaido University Graduate School of Medicine, Sapporo, JapanDepartment of Neurosurgery, Hokkaido University Graduate School of Medicine, Sapporo, JapanDepartment of Neurosurgery, Hokkaido University Graduate School of Medicine, Sapporo, JapanDepartment of Neurosurgery, Hokkaido University Graduate School of Medicine, Sapporo, JapanDepartment of Neurology and Stroke Center, National Taiwan University Hospital, Taipei, TaiwanDepartment of Neurosurgery, Hokkaido University Graduate School of Medicine, Sapporo, JapanObjectiveBrain-derived neurotrophic factor (BDNF) is a pivotal growth factor for neuronal survival; however, its precise role following intracerebral hemorrhage (ICH) remains poorly understood. This study sought to investigate whether intraventricular administration of BDNF could enhance neurogenesis and ameliorate neuroinflammation, resulting in improvement of neurological outcomes in a rat ICH model.MethodsMale Sprague–Dawley rats were utilized to create an acute ICH model by collagenase infusion into the internal capsule. An intraventricular minipump was subcutaneously implanted, with the catheter tip inserted into the contralateral ventricle to deliver BDNF for 7 consecutive days. The rats were assigned to one of three experimental groups; the BDNF group, the anti-BDNF antibody group, and the sham group. Functional assessment was conducted up to 14 days post-ICH, and immunohistochemical analysis was performed to evaluate neurogenesis, apoptosis, and neuroinflammation in the perihematomal area and the subventricular zone (SVZ).ResultsBrain-derived neurotrophic factor treatment significantly increased the proliferation of neural stem cells in the perihematomal region. It also reduced the neuroinflammation 14 days post-ICH. Additionally, BDNF treatment demonstrated a favorable neurological function at 14 days post-ICH.ConclusionIntraventricular administration of BDNF demonstrated favorable recovery after ICH by reducing inflammation and enhancing neurogenesis.https://www.frontiersin.org/articles/10.3389/fneur.2025.1606606/fullintracerebral hemorrhagebrain derived neurotrophic factorintraventricular transplantationinflammationneurogenesis |
| spellingShingle | Ting-Chun Lin Masahito Kawabori Erika Yoshie Yo Nakahara Li-Kai Tsai Miki Fujimura Intraventricular continuous BDNF administration ameliorates neuroinflammation and enhances neurogenesis against rodent intracerebral hemorrhage model Frontiers in Neurology intracerebral hemorrhage brain derived neurotrophic factor intraventricular transplantation inflammation neurogenesis |
| title | Intraventricular continuous BDNF administration ameliorates neuroinflammation and enhances neurogenesis against rodent intracerebral hemorrhage model |
| title_full | Intraventricular continuous BDNF administration ameliorates neuroinflammation and enhances neurogenesis against rodent intracerebral hemorrhage model |
| title_fullStr | Intraventricular continuous BDNF administration ameliorates neuroinflammation and enhances neurogenesis against rodent intracerebral hemorrhage model |
| title_full_unstemmed | Intraventricular continuous BDNF administration ameliorates neuroinflammation and enhances neurogenesis against rodent intracerebral hemorrhage model |
| title_short | Intraventricular continuous BDNF administration ameliorates neuroinflammation and enhances neurogenesis against rodent intracerebral hemorrhage model |
| title_sort | intraventricular continuous bdnf administration ameliorates neuroinflammation and enhances neurogenesis against rodent intracerebral hemorrhage model |
| topic | intracerebral hemorrhage brain derived neurotrophic factor intraventricular transplantation inflammation neurogenesis |
| url | https://www.frontiersin.org/articles/10.3389/fneur.2025.1606606/full |
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