Antitumor Molecular Mechanism of Chlorogenic Acid on Inducting Genes GSK-3β and APC and Inhibiting Gene β-Catenin
Objective. Inhibiting gene β-catenin and inducting genes GSK-3β and APC, promoting the tumor cell apoptosis in Wnt pathway, by chlorogenic acid were discussed (CGA). Method. The different genes were scanned by the 4*44K mouse microarray chips. The effect of the three genes was confirmed by RT-PCR t...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/951319 |
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| _version_ | 1832547470170652672 |
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| author | Ruoshi Xu Qiumei Kang Jie Ren Zukun Li Xiaoping Xu |
| author_facet | Ruoshi Xu Qiumei Kang Jie Ren Zukun Li Xiaoping Xu |
| author_sort | Ruoshi Xu |
| collection | DOAJ |
| description | Objective. Inhibiting gene β-catenin and inducting genes GSK-3β and APC, promoting the tumor cell apoptosis in Wnt pathway, by chlorogenic acid were discussed (CGA). Method. The different genes were scanned by the 4*44K mouse microarray chips. The effect of the three genes was confirmed by RT-PCR technique with CGA dosage of 5, 10, and 20 mg/kg. Result. The expression of GSK-3β and APC was upregulated in group of 20 mg/kg dosage (P<0.05) and the expression of β-catenin was downregulated in the same dosage (P<0.05). Conclusion. The results infer that the multimeric protein complex of β-catenin could be increased by CGA upregulated genes GSK-3β and APC, which could inhibit the free β-catenin into the nucleus to connect with TCF. So the transcriptional expression of the target genes will be cut to abnormal cell proliferation. It is probably one of the ways that can stop the tumor increase by CGA. |
| format | Article |
| id | doaj-art-e173070b61744ef580be4d3b46bfdb5a |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-e173070b61744ef580be4d3b46bfdb5a2025-02-03T06:44:39ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732013-01-01201310.1155/2013/951319951319Antitumor Molecular Mechanism of Chlorogenic Acid on Inducting Genes GSK-3β and APC and Inhibiting Gene β-CateninRuoshi Xu0Qiumei Kang1Jie Ren2Zukun Li3Xiaoping Xu4West China School of Stomatology, Sichuan University, Chengdu, Sichuan 610041, ChinaWest China School of Pharmacy, Sichuan University, Chengdu, Sichuan 610041, ChinaWest China School of Pharmacy, Sichuan University, Chengdu, Sichuan 610041, ChinaWest China School of Pharmacy, Sichuan University, Chengdu, Sichuan 610041, ChinaWest China School of Pharmacy, Sichuan University, Chengdu, Sichuan 610041, ChinaObjective. Inhibiting gene β-catenin and inducting genes GSK-3β and APC, promoting the tumor cell apoptosis in Wnt pathway, by chlorogenic acid were discussed (CGA). Method. The different genes were scanned by the 4*44K mouse microarray chips. The effect of the three genes was confirmed by RT-PCR technique with CGA dosage of 5, 10, and 20 mg/kg. Result. The expression of GSK-3β and APC was upregulated in group of 20 mg/kg dosage (P<0.05) and the expression of β-catenin was downregulated in the same dosage (P<0.05). Conclusion. The results infer that the multimeric protein complex of β-catenin could be increased by CGA upregulated genes GSK-3β and APC, which could inhibit the free β-catenin into the nucleus to connect with TCF. So the transcriptional expression of the target genes will be cut to abnormal cell proliferation. It is probably one of the ways that can stop the tumor increase by CGA.http://dx.doi.org/10.1155/2013/951319 |
| spellingShingle | Ruoshi Xu Qiumei Kang Jie Ren Zukun Li Xiaoping Xu Antitumor Molecular Mechanism of Chlorogenic Acid on Inducting Genes GSK-3β and APC and Inhibiting Gene β-Catenin Journal of Analytical Methods in Chemistry |
| title | Antitumor Molecular Mechanism of Chlorogenic Acid on Inducting Genes GSK-3β and APC and Inhibiting Gene β-Catenin |
| title_full | Antitumor Molecular Mechanism of Chlorogenic Acid on Inducting Genes GSK-3β and APC and Inhibiting Gene β-Catenin |
| title_fullStr | Antitumor Molecular Mechanism of Chlorogenic Acid on Inducting Genes GSK-3β and APC and Inhibiting Gene β-Catenin |
| title_full_unstemmed | Antitumor Molecular Mechanism of Chlorogenic Acid on Inducting Genes GSK-3β and APC and Inhibiting Gene β-Catenin |
| title_short | Antitumor Molecular Mechanism of Chlorogenic Acid on Inducting Genes GSK-3β and APC and Inhibiting Gene β-Catenin |
| title_sort | antitumor molecular mechanism of chlorogenic acid on inducting genes gsk 3β and apc and inhibiting gene β catenin |
| url | http://dx.doi.org/10.1155/2013/951319 |
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