Blue Light and Green Light Fundus Autofluorescence, Complementary to Optical Coherence Tomography, in Age-Related Macular Degeneration Evaluation

<b>Background:</b> Age-related macular degeneration (AMD) is one of the leading causes of permanent vision loss in the elderly, particularly in higher-income countries. Fundus autofluorescence (FAF) imaging is a widely used, non-invasive technique that complements structural imaging in t...

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Main Authors: Antonia-Elena Ranetti, Horia Tudor Stanca, Mihnea Munteanu, Raluca Bievel Radulescu, Simona Stanca
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Diagnostics
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Online Access:https://www.mdpi.com/2075-4418/15/13/1688
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author Antonia-Elena Ranetti
Horia Tudor Stanca
Mihnea Munteanu
Raluca Bievel Radulescu
Simona Stanca
author_facet Antonia-Elena Ranetti
Horia Tudor Stanca
Mihnea Munteanu
Raluca Bievel Radulescu
Simona Stanca
author_sort Antonia-Elena Ranetti
collection DOAJ
description <b>Background:</b> Age-related macular degeneration (AMD) is one of the leading causes of permanent vision loss in the elderly, particularly in higher-income countries. Fundus autofluorescence (FAF) imaging is a widely used, non-invasive technique that complements structural imaging in the assessment of retinal pigment epithelium (RPE) integrity. While optical coherence tomography (OCT) remains the gold standard for retinal imaging due to its high-resolution cross-sectional visualization, FAF offers unique metabolic insights. Among the FAF modalities, blue light FAF (B-FAF) is more commonly employed, whereas green light FAF (G-FAF) provides subtly different image characteristics, particularly improved visualization and contrast in the central macula. Despite identical acquisition times and nearly indistinguishable workflows, G-FAF is notably underutilized in clinical practice. <b>Objectives:</b> This narrative review critically compares green and blue FAF in terms of their diagnostic utility relative to OCT, with a focus on lesion detectability, macular pigment interference, and clinical decision-making in retinal disorders. <b>Methods:</b> A comprehensive literature search was performed using the PubMed database for studies published prior to February 2025. The search utilized the keywords fundus autofluorescence and age-related macular degeneration. The primary focus was on short-wavelength FAF and its clinical utility in AMD, considering three aspects: diagnosis, follow-up, and prognosis. The OCT findings served as the reference standard for anatomical correlation and diagnostic accuracy. <b>Results:</b> Both FAF modalities correlated well with OCT in detecting RPE abnormalities. G-FAF demonstrated improved visibility of central lesions due to reduced masking by macular pigment and enhanced contrast in the macula. However, clinical preference remained skewed toward B-FAF, driven more by tradition and device default settings than by evidence-based superiority. G-FAF’s diagnostic potential remains underrecognized despite its comparable practicality and subtle imaging advantages specifically for AMD patients. AMD stages were accurately characterized, and relevant images were used to highlight the significance of G-FAF and B-FAF in the examination of AMD patients. <b>Conclusions:</b> While OCT remains the gold standard, FAF provides complementary information that can guide management strategy. Since G-FAF is functionally equivalent in acquisition, it offers slight advantages. Broader awareness and more frequent integration of G-FAF that could optimize multimodal imaging strategies, particularly in the intermediate stage, should be developed.
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spelling doaj-art-e16c4e0cadef459fabc37eef17eda4142025-08-20T02:35:46ZengMDPI AGDiagnostics2075-44182025-07-011513168810.3390/diagnostics15131688Blue Light and Green Light Fundus Autofluorescence, Complementary to Optical Coherence Tomography, in Age-Related Macular Degeneration EvaluationAntonia-Elena Ranetti0Horia Tudor Stanca1Mihnea Munteanu2Raluca Bievel Radulescu3Simona Stanca4Doctoral School, “Carol Davila” University of Medicine and Pharmacy, Strada Dionisie Lupu No. 37, 020021 București, RomaniaDoctoral School, “Carol Davila” University of Medicine and Pharmacy, Strada Dionisie Lupu No. 37, 020021 București, RomaniaDepartment of Ophthalmology, “Victor Babes” University of Medicine and Pharmacy, 300041 Timisoara, RomaniaDoctoral School, “Carol Davila” University of Medicine and Pharmacy, Strada Dionisie Lupu No. 37, 020021 București, RomaniaClinical Department of Pediatrics, “Carol Davila” University of Medicine and Pharmacy, Strada Dionisie Lupu No. 37, 020021 București, Romania<b>Background:</b> Age-related macular degeneration (AMD) is one of the leading causes of permanent vision loss in the elderly, particularly in higher-income countries. Fundus autofluorescence (FAF) imaging is a widely used, non-invasive technique that complements structural imaging in the assessment of retinal pigment epithelium (RPE) integrity. While optical coherence tomography (OCT) remains the gold standard for retinal imaging due to its high-resolution cross-sectional visualization, FAF offers unique metabolic insights. Among the FAF modalities, blue light FAF (B-FAF) is more commonly employed, whereas green light FAF (G-FAF) provides subtly different image characteristics, particularly improved visualization and contrast in the central macula. Despite identical acquisition times and nearly indistinguishable workflows, G-FAF is notably underutilized in clinical practice. <b>Objectives:</b> This narrative review critically compares green and blue FAF in terms of their diagnostic utility relative to OCT, with a focus on lesion detectability, macular pigment interference, and clinical decision-making in retinal disorders. <b>Methods:</b> A comprehensive literature search was performed using the PubMed database for studies published prior to February 2025. The search utilized the keywords fundus autofluorescence and age-related macular degeneration. The primary focus was on short-wavelength FAF and its clinical utility in AMD, considering three aspects: diagnosis, follow-up, and prognosis. The OCT findings served as the reference standard for anatomical correlation and diagnostic accuracy. <b>Results:</b> Both FAF modalities correlated well with OCT in detecting RPE abnormalities. G-FAF demonstrated improved visibility of central lesions due to reduced masking by macular pigment and enhanced contrast in the macula. However, clinical preference remained skewed toward B-FAF, driven more by tradition and device default settings than by evidence-based superiority. G-FAF’s diagnostic potential remains underrecognized despite its comparable practicality and subtle imaging advantages specifically for AMD patients. AMD stages were accurately characterized, and relevant images were used to highlight the significance of G-FAF and B-FAF in the examination of AMD patients. <b>Conclusions:</b> While OCT remains the gold standard, FAF provides complementary information that can guide management strategy. Since G-FAF is functionally equivalent in acquisition, it offers slight advantages. Broader awareness and more frequent integration of G-FAF that could optimize multimodal imaging strategies, particularly in the intermediate stage, should be developed.https://www.mdpi.com/2075-4418/15/13/1688age-related macular degenerationfundus autofluorescenceoptical coherence tomographyretinal pigment epitheliumscanning laser ophthalmoscopemultimodal imaging
spellingShingle Antonia-Elena Ranetti
Horia Tudor Stanca
Mihnea Munteanu
Raluca Bievel Radulescu
Simona Stanca
Blue Light and Green Light Fundus Autofluorescence, Complementary to Optical Coherence Tomography, in Age-Related Macular Degeneration Evaluation
Diagnostics
age-related macular degeneration
fundus autofluorescence
optical coherence tomography
retinal pigment epithelium
scanning laser ophthalmoscope
multimodal imaging
title Blue Light and Green Light Fundus Autofluorescence, Complementary to Optical Coherence Tomography, in Age-Related Macular Degeneration Evaluation
title_full Blue Light and Green Light Fundus Autofluorescence, Complementary to Optical Coherence Tomography, in Age-Related Macular Degeneration Evaluation
title_fullStr Blue Light and Green Light Fundus Autofluorescence, Complementary to Optical Coherence Tomography, in Age-Related Macular Degeneration Evaluation
title_full_unstemmed Blue Light and Green Light Fundus Autofluorescence, Complementary to Optical Coherence Tomography, in Age-Related Macular Degeneration Evaluation
title_short Blue Light and Green Light Fundus Autofluorescence, Complementary to Optical Coherence Tomography, in Age-Related Macular Degeneration Evaluation
title_sort blue light and green light fundus autofluorescence complementary to optical coherence tomography in age related macular degeneration evaluation
topic age-related macular degeneration
fundus autofluorescence
optical coherence tomography
retinal pigment epithelium
scanning laser ophthalmoscope
multimodal imaging
url https://www.mdpi.com/2075-4418/15/13/1688
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