Characterization of size distribution and markers for mosquito extracellular vesicles
Extracellular vesicles (EVs) are non-replicative, cell-derived membranous structures secreted by potentially all eukaryotic cells, playing a crucial role in intercellular communication. The study of EVs requires approaches and tools, which have predominantly been developed for mammalian models. Here...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-04-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2025.1497795/full |
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| author | Félix Rey-Cadilhac Florian Rachenne Antonin Marquant Josephine Lai Kee Him Aurélie Ancelin Veronica Foisor Marie Morille Marie Morille Sébastien Lyonnais Chantal Cazevieille Dorothée Missé Julien Pompon |
| author_facet | Félix Rey-Cadilhac Florian Rachenne Antonin Marquant Josephine Lai Kee Him Aurélie Ancelin Veronica Foisor Marie Morille Marie Morille Sébastien Lyonnais Chantal Cazevieille Dorothée Missé Julien Pompon |
| author_sort | Félix Rey-Cadilhac |
| collection | DOAJ |
| description | Extracellular vesicles (EVs) are non-replicative, cell-derived membranous structures secreted by potentially all eukaryotic cells, playing a crucial role in intercellular communication. The study of EVs requires approaches and tools, which have predominantly been developed for mammalian models. Here, we undertook a multimodal characterization of mosquito EVs to provide a technical and knowledge foundation for their study. First, using a cell line model from Aedes aegypti and applying multiple analytical technologies (i.e., NTA, TEM, cryo-EM, and AFM), we observed that mosquito EVs range from 20 to 500 nm in diameter and that a majority are smaller than 100 nm. Second, we showed that smaller EVs are secreted in mosquito saliva. Third, we evaluated the capacity of differential centrifugation and size exclusion chromatography to separate mosquito EVs, revealing the strengths and weaknesses of each technology. Finally, we identified a mosquito homolog of CD63 as an extravesicular marker and the mosquito syntenin as a putative luminal marker. Overall, our results promote the development of tools and approaches for the study of mosquito EVs. |
| format | Article |
| id | doaj-art-e1614c5bd70b44e69ddff559fa268677 |
| institution | DOAJ |
| issn | 2296-634X |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj-art-e1614c5bd70b44e69ddff559fa2686772025-08-20T03:06:30ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2025-04-011310.3389/fcell.2025.14977951497795Characterization of size distribution and markers for mosquito extracellular vesiclesFélix Rey-Cadilhac0Florian Rachenne1Antonin Marquant2Josephine Lai Kee Him3Aurélie Ancelin4Veronica Foisor5Marie Morille6Marie Morille7Sébastien Lyonnais8Chantal Cazevieille9Dorothée Missé10Julien Pompon11MIVEGEC, Univ. Montpellier, IRD, CNRS, Montpellier, FranceMIVEGEC, Univ. Montpellier, IRD, CNRS, Montpellier, FranceICGM, Univ. Montpellier, CNRS, ENSCM, Montpellier, FranceCBS (Centre de Biologie Structurale), Univ. Montpellier, CNRS, Inserm, Montpellier, FranceCBS (Centre de Biologie Structurale), Univ. Montpellier, CNRS, Inserm, Montpellier, FranceUnchained Labs, Royston, United KingdomICGM, Univ. Montpellier, CNRS, ENSCM, Montpellier, FranceInstitut Universitaire de France (IUF), Paris, FranceCEMIPAI, UAR3725 CNRS - Montpellier University, Montpellier, FranceINM (Institut de Neuroscience de Montpellier), Electronic Microscopy Plateform, Saint Eloi Hospital, Montpellier, FranceMIVEGEC, Univ. Montpellier, IRD, CNRS, Montpellier, FranceMIVEGEC, Univ. Montpellier, IRD, CNRS, Montpellier, FranceExtracellular vesicles (EVs) are non-replicative, cell-derived membranous structures secreted by potentially all eukaryotic cells, playing a crucial role in intercellular communication. The study of EVs requires approaches and tools, which have predominantly been developed for mammalian models. Here, we undertook a multimodal characterization of mosquito EVs to provide a technical and knowledge foundation for their study. First, using a cell line model from Aedes aegypti and applying multiple analytical technologies (i.e., NTA, TEM, cryo-EM, and AFM), we observed that mosquito EVs range from 20 to 500 nm in diameter and that a majority are smaller than 100 nm. Second, we showed that smaller EVs are secreted in mosquito saliva. Third, we evaluated the capacity of differential centrifugation and size exclusion chromatography to separate mosquito EVs, revealing the strengths and weaknesses of each technology. Finally, we identified a mosquito homolog of CD63 as an extravesicular marker and the mosquito syntenin as a putative luminal marker. Overall, our results promote the development of tools and approaches for the study of mosquito EVs.https://www.frontiersin.org/articles/10.3389/fcell.2025.1497795/fullextracellular vesiclesmosquitomicroscopyprotein markerssyntenintetraspanin |
| spellingShingle | Félix Rey-Cadilhac Florian Rachenne Antonin Marquant Josephine Lai Kee Him Aurélie Ancelin Veronica Foisor Marie Morille Marie Morille Sébastien Lyonnais Chantal Cazevieille Dorothée Missé Julien Pompon Characterization of size distribution and markers for mosquito extracellular vesicles Frontiers in Cell and Developmental Biology extracellular vesicles mosquito microscopy protein markers syntenin tetraspanin |
| title | Characterization of size distribution and markers for mosquito extracellular vesicles |
| title_full | Characterization of size distribution and markers for mosquito extracellular vesicles |
| title_fullStr | Characterization of size distribution and markers for mosquito extracellular vesicles |
| title_full_unstemmed | Characterization of size distribution and markers for mosquito extracellular vesicles |
| title_short | Characterization of size distribution and markers for mosquito extracellular vesicles |
| title_sort | characterization of size distribution and markers for mosquito extracellular vesicles |
| topic | extracellular vesicles mosquito microscopy protein markers syntenin tetraspanin |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2025.1497795/full |
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