Gut microbiota characteristics in neonatal respiratory distress syndrome and the therapeutic potential of probiotics in recovery
BackgroundNeonatal Respiratory Distress Syndrome (NRDS) is a common and severe respiratory disorder in neonates, particularly among preterm infants (PTIs), and is often associated with hypoxemia and multiple organ dysfunction. This study aims to investigate the gut microbiota characteristics in NRDS...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-04-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1544055/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849766555004436480 |
|---|---|
| author | Yongcheng Fu Xiujuan Wang Lintao Nie Zhaorui Wang Xiao Ma Lijia Wu Liping Han Wenjun Fu Ruoming Wang Hongyan Ren Da Zhang Juan Ding |
| author_facet | Yongcheng Fu Xiujuan Wang Lintao Nie Zhaorui Wang Xiao Ma Lijia Wu Liping Han Wenjun Fu Ruoming Wang Hongyan Ren Da Zhang Juan Ding |
| author_sort | Yongcheng Fu |
| collection | DOAJ |
| description | BackgroundNeonatal Respiratory Distress Syndrome (NRDS) is a common and severe respiratory disorder in neonates, particularly among preterm infants (PTIs), and is often associated with hypoxemia and multiple organ dysfunction. This study aims to investigate the gut microbiota characteristics in NRDS and the potential regulatory role of probiotics in restoring gut microbiota dysbiosis.MethodsThis study enrolled 55 PTIs diagnosed with NRDS and 26 preterm infants without NRDS. The NRDS group was classified into two groups based on treatment: an antibiotic-only group (TA group, N = 30) and an antibiotic plus probiotics group (TB group, N = 25). Fecal samples were collected within 48 h of birth and again after recovery, for 16S rRNA sequencing.ResultsThe study revealed that the gut microbiota diversity in the NRDS group was significantly greater than in the non-NRDS group, and the microbiota composition in the NRDS group was closely associated with multiple clinical indicators, including Apgar score, pH, PaO2, and PaCO2. Notably, the abundance of bacteria such as Muribaculaceae Incertae Sedis, Rhodococcus, and Corynebacterium was significantly higher in the NRDS group, which may contribute to disease progression. ROC analysis suggested that gut microbiota could serve as potential biomarkers for diagnosing NRDS. Probiotic intervention notably restored the gut microbiota structure in the NRDS group, particularly by enhancing the abundance of beneficial genera such as Streptococcus, Bifidobacterium, and Clostridium. This intervention reduced the microbiota disparity between the NRDS group and normal one-month-old children, thereby slowing disease progression.ConclusionThis study demonstrated that the NRDS displayed an increase in gut microbiota diversity and alterations in specific bacterial populations, both of which were closely correlated with clinical data. Probiotic treatment aids in restoring the disrupted gut microbiota in NRDS infants, promoting disease recovery, and providing new biomarkers and clinical strategies for managing NRDS. |
| format | Article |
| id | doaj-art-e15938ee48e142ff90b13e6913458f5a |
| institution | DOAJ |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-e15938ee48e142ff90b13e6913458f5a2025-08-20T03:04:31ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-04-011610.3389/fmicb.2025.15440551544055Gut microbiota characteristics in neonatal respiratory distress syndrome and the therapeutic potential of probiotics in recoveryYongcheng Fu0Xiujuan Wang1Lintao Nie2Zhaorui Wang3Xiao Ma4Lijia Wu5Liping Han6Wenjun Fu7Ruoming Wang8Hongyan Ren9Da Zhang10Juan Ding11Department of Pediatric Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Nursing, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Nursing, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaTranslational Medicine Research Center, The Fifth Clinical Medical College of Henan University of Chinese Medicine (Zhengzhou People's Hospital), Zhengzhou, ChinaDepartment of Human Resources, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Neonatal Intensive Care Unit, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Obstetrics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Nursing, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaShanghai Mobio Biomedical Technology Co., Ltd., Shanghai, ChinaDepartment of Pediatric Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Nursing, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaBackgroundNeonatal Respiratory Distress Syndrome (NRDS) is a common and severe respiratory disorder in neonates, particularly among preterm infants (PTIs), and is often associated with hypoxemia and multiple organ dysfunction. This study aims to investigate the gut microbiota characteristics in NRDS and the potential regulatory role of probiotics in restoring gut microbiota dysbiosis.MethodsThis study enrolled 55 PTIs diagnosed with NRDS and 26 preterm infants without NRDS. The NRDS group was classified into two groups based on treatment: an antibiotic-only group (TA group, N = 30) and an antibiotic plus probiotics group (TB group, N = 25). Fecal samples were collected within 48 h of birth and again after recovery, for 16S rRNA sequencing.ResultsThe study revealed that the gut microbiota diversity in the NRDS group was significantly greater than in the non-NRDS group, and the microbiota composition in the NRDS group was closely associated with multiple clinical indicators, including Apgar score, pH, PaO2, and PaCO2. Notably, the abundance of bacteria such as Muribaculaceae Incertae Sedis, Rhodococcus, and Corynebacterium was significantly higher in the NRDS group, which may contribute to disease progression. ROC analysis suggested that gut microbiota could serve as potential biomarkers for diagnosing NRDS. Probiotic intervention notably restored the gut microbiota structure in the NRDS group, particularly by enhancing the abundance of beneficial genera such as Streptococcus, Bifidobacterium, and Clostridium. This intervention reduced the microbiota disparity between the NRDS group and normal one-month-old children, thereby slowing disease progression.ConclusionThis study demonstrated that the NRDS displayed an increase in gut microbiota diversity and alterations in specific bacterial populations, both of which were closely correlated with clinical data. Probiotic treatment aids in restoring the disrupted gut microbiota in NRDS infants, promoting disease recovery, and providing new biomarkers and clinical strategies for managing NRDS.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1544055/full16s rRNA sequencingneonatal respiratory distress syndromegut microbiotaprobioticspreterm infantspredictive diagnosis |
| spellingShingle | Yongcheng Fu Xiujuan Wang Lintao Nie Zhaorui Wang Xiao Ma Lijia Wu Liping Han Wenjun Fu Ruoming Wang Hongyan Ren Da Zhang Juan Ding Gut microbiota characteristics in neonatal respiratory distress syndrome and the therapeutic potential of probiotics in recovery Frontiers in Microbiology 16s rRNA sequencing neonatal respiratory distress syndrome gut microbiota probiotics preterm infants predictive diagnosis |
| title | Gut microbiota characteristics in neonatal respiratory distress syndrome and the therapeutic potential of probiotics in recovery |
| title_full | Gut microbiota characteristics in neonatal respiratory distress syndrome and the therapeutic potential of probiotics in recovery |
| title_fullStr | Gut microbiota characteristics in neonatal respiratory distress syndrome and the therapeutic potential of probiotics in recovery |
| title_full_unstemmed | Gut microbiota characteristics in neonatal respiratory distress syndrome and the therapeutic potential of probiotics in recovery |
| title_short | Gut microbiota characteristics in neonatal respiratory distress syndrome and the therapeutic potential of probiotics in recovery |
| title_sort | gut microbiota characteristics in neonatal respiratory distress syndrome and the therapeutic potential of probiotics in recovery |
| topic | 16s rRNA sequencing neonatal respiratory distress syndrome gut microbiota probiotics preterm infants predictive diagnosis |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1544055/full |
| work_keys_str_mv | AT yongchengfu gutmicrobiotacharacteristicsinneonatalrespiratorydistresssyndromeandthetherapeuticpotentialofprobioticsinrecovery AT xiujuanwang gutmicrobiotacharacteristicsinneonatalrespiratorydistresssyndromeandthetherapeuticpotentialofprobioticsinrecovery AT lintaonie gutmicrobiotacharacteristicsinneonatalrespiratorydistresssyndromeandthetherapeuticpotentialofprobioticsinrecovery AT zhaoruiwang gutmicrobiotacharacteristicsinneonatalrespiratorydistresssyndromeandthetherapeuticpotentialofprobioticsinrecovery AT xiaoma gutmicrobiotacharacteristicsinneonatalrespiratorydistresssyndromeandthetherapeuticpotentialofprobioticsinrecovery AT lijiawu gutmicrobiotacharacteristicsinneonatalrespiratorydistresssyndromeandthetherapeuticpotentialofprobioticsinrecovery AT lipinghan gutmicrobiotacharacteristicsinneonatalrespiratorydistresssyndromeandthetherapeuticpotentialofprobioticsinrecovery AT wenjunfu gutmicrobiotacharacteristicsinneonatalrespiratorydistresssyndromeandthetherapeuticpotentialofprobioticsinrecovery AT ruomingwang gutmicrobiotacharacteristicsinneonatalrespiratorydistresssyndromeandthetherapeuticpotentialofprobioticsinrecovery AT hongyanren gutmicrobiotacharacteristicsinneonatalrespiratorydistresssyndromeandthetherapeuticpotentialofprobioticsinrecovery AT dazhang gutmicrobiotacharacteristicsinneonatalrespiratorydistresssyndromeandthetherapeuticpotentialofprobioticsinrecovery AT juanding gutmicrobiotacharacteristicsinneonatalrespiratorydistresssyndromeandthetherapeuticpotentialofprobioticsinrecovery |