Bioinformatics study and cytotoxicity of several curcumin analogues in ovarian cancer

Ovarian cancer ranks as Indonesia’s third-leading cause of cancer-related death, emphasising the need for innovative treatments. This study combined bioinformatics, molecular docking, and experimental assays to tackle this challenge. We identified 166 ovarian cancer-related genes, with MYC standing...

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Main Authors: Retno Murwanti, Ritmaleni, Navista Sri Octa Ujiantari, I Made Rhamandana Putra, Aliffian Farhan Wahyudi, Vigha Ilmanafi Arifka
Format: Article
Language:English
Published: Elsevier 2025-01-01
Series:Current Research in Toxicology
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666027X25000167
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author Retno Murwanti
Ritmaleni
Navista Sri Octa Ujiantari
I Made Rhamandana Putra
Aliffian Farhan Wahyudi
Vigha Ilmanafi Arifka
author_facet Retno Murwanti
Ritmaleni
Navista Sri Octa Ujiantari
I Made Rhamandana Putra
Aliffian Farhan Wahyudi
Vigha Ilmanafi Arifka
author_sort Retno Murwanti
collection DOAJ
description Ovarian cancer ranks as Indonesia’s third-leading cause of cancer-related death, emphasising the need for innovative treatments. This study combined bioinformatics, molecular docking, and experimental assays to tackle this challenge. We identified 166 ovarian cancer-related genes, with MYC standing out as a key target. Analysis of MYC mutations revealed prevalent alterations, though no significant survival differences were observed in patients with or without the mutations. Molecular docking pinpointed compound B155 as a promising MYC inhibitor. A preliminary cytotoxicity assay revealed compound B155′s notable activity, with an 87.19 % inhibition of cell viability at 50 μM. Most of the other curcumin analogues only caused more than 50 % inhibition at the same concentration. This result suggests alternative mechanisms of action, possibly antioxidant effects, warranting further exploration. In summary, this study unveiled MYC as a prime target for ovarian cancer treatment, with curcumin analogues like B155 showing potential. Nonetheless, the complex factors affecting cytotoxicity underscore the need for deeper investigation into these compounds’ mechanisms in ovarian cancer cells.
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spelling doaj-art-e14b5db720e64176ab5759b92e602a3d2025-08-20T02:03:14ZengElsevierCurrent Research in Toxicology2666-027X2025-01-01810023010.1016/j.crtox.2025.100230Bioinformatics study and cytotoxicity of several curcumin analogues in ovarian cancerRetno Murwanti0 Ritmaleni1Navista Sri Octa Ujiantari2I Made Rhamandana Putra3Aliffian Farhan Wahyudi4Vigha Ilmanafi Arifka5Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia; Curcumin Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia; Corresponding author at: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia.Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia; Curcumin Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia; Curcumin Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaFaculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaFaculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaFaculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaOvarian cancer ranks as Indonesia’s third-leading cause of cancer-related death, emphasising the need for innovative treatments. This study combined bioinformatics, molecular docking, and experimental assays to tackle this challenge. We identified 166 ovarian cancer-related genes, with MYC standing out as a key target. Analysis of MYC mutations revealed prevalent alterations, though no significant survival differences were observed in patients with or without the mutations. Molecular docking pinpointed compound B155 as a promising MYC inhibitor. A preliminary cytotoxicity assay revealed compound B155′s notable activity, with an 87.19 % inhibition of cell viability at 50 μM. Most of the other curcumin analogues only caused more than 50 % inhibition at the same concentration. This result suggests alternative mechanisms of action, possibly antioxidant effects, warranting further exploration. In summary, this study unveiled MYC as a prime target for ovarian cancer treatment, with curcumin analogues like B155 showing potential. Nonetheless, the complex factors affecting cytotoxicity underscore the need for deeper investigation into these compounds’ mechanisms in ovarian cancer cells.http://www.sciencedirect.com/science/article/pii/S2666027X25000167Ovarian cancerCurcumin analoguesDibenzylidene-cyclopentanoneCytotoxicityBioinformatics
spellingShingle Retno Murwanti
Ritmaleni
Navista Sri Octa Ujiantari
I Made Rhamandana Putra
Aliffian Farhan Wahyudi
Vigha Ilmanafi Arifka
Bioinformatics study and cytotoxicity of several curcumin analogues in ovarian cancer
Current Research in Toxicology
Ovarian cancer
Curcumin analogues
Dibenzylidene-cyclopentanone
Cytotoxicity
Bioinformatics
title Bioinformatics study and cytotoxicity of several curcumin analogues in ovarian cancer
title_full Bioinformatics study and cytotoxicity of several curcumin analogues in ovarian cancer
title_fullStr Bioinformatics study and cytotoxicity of several curcumin analogues in ovarian cancer
title_full_unstemmed Bioinformatics study and cytotoxicity of several curcumin analogues in ovarian cancer
title_short Bioinformatics study and cytotoxicity of several curcumin analogues in ovarian cancer
title_sort bioinformatics study and cytotoxicity of several curcumin analogues in ovarian cancer
topic Ovarian cancer
Curcumin analogues
Dibenzylidene-cyclopentanone
Cytotoxicity
Bioinformatics
url http://www.sciencedirect.com/science/article/pii/S2666027X25000167
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