Screening of a microbial consortium with efficient corn stover degradation ability at low temperature
To speed up the degradation of corn stover directly returned to soil at low temperature, the corn stover-degrading microbial consortium GF-20, acclimated to biological decomposition in the frigid region, was successfully constructed under a long-term limiting substrate. To evaluate its potential in...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2016-10-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311915612722 |
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| author | Qinggeer Ju-lin GAO Xiao-fang YU Bao-lin ZHANG Zhi-gang WANG Borjigin Naoganchaolu Shu-ping HU Ji-ying SUN Min XIE Zhen WANG |
| author_facet | Qinggeer Ju-lin GAO Xiao-fang YU Bao-lin ZHANG Zhi-gang WANG Borjigin Naoganchaolu Shu-ping HU Ji-ying SUN Min XIE Zhen WANG |
| author_sort | Qinggeer |
| collection | DOAJ |
| description | To speed up the degradation of corn stover directly returned to soil at low temperature, the corn stover-degrading microbial consortium GF-20, acclimated to biological decomposition in the frigid region, was successfully constructed under a long-term limiting substrate. To evaluate its potential in accelerating the decomposition of un-pretreated corn stover, the decomposing property, fermentation dynamic and the microbial diversity were analyzed. GF-20 degraded corn stover by 32% after 15-day fermentation at 10°C. Peak activities of filter paperlyase (FPA), β-glucosidases (CB), endoglucanases (Cx), and cellobiohydrolases (C1) were 1.15, 1.67, 1.73, and 1.42 U mL−1, appearing at the 6th, 3rd, 11th, and 9th d, respectively. The pH averaged at 6.73–8.42, and the optical density (OD) value peaked at 1.87 at the 120 h of the degradation process. Cellulase, hemicellulase and lignin in corn stover were persistently degraded by 44.85, 43.85 and 25.29% at the end of incubation. Result of denaturing gradient gel electrophoresis (DGGE) profiles demonstrated that GF-20 had a stable component structure under switching the temperature and pH. The composition of the GF-20 was also analyzed by constructing bacterial 16S rDNA clone library and fungal 18SrDNA-PCR-DGGE. Twenty-two bacterial clones and four fungal bands were detected and identified dominant bacteria represented by Cellvibrio mixtus subsp., Azospira oryzae, Arcobacter defluyii, and Clostridium populeti and the fungi were mainly identified as related to Trichosporon sp. |
| format | Article |
| id | doaj-art-e146d58a1c04487b843a7145f4142104 |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2016-10-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-e146d58a1c04487b843a7145f41421042025-08-20T03:58:03ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192016-10-0115102369237910.1016/S2095-3119(15)61272-2Screening of a microbial consortium with efficient corn stover degradation ability at low temperature Qinggeer0Ju-lin GAO1Xiao-fang YU2Bao-lin ZHANG3Zhi-gang WANG4Borjigin Naoganchaolu5Shu-ping HU6Ji-ying SUN7Min XIE8Zhen WANG9Agricultural College, Inner Mongolia Agricultural University, Hohhot 010019, P.R. ChinaAgricultural College, Inner Mongolia Agricultural University, Hohhot 010019, P.R. China; Correspondence GAO Ju-lin, Tel: +86-471-4301317Agricultural College, Inner Mongolia Agricultural University, Hohhot 010019, P.R. ChinaChemistry and Environmental Science College, Inner Mongolia Normal University, Hohhot 010019, P.R. ChinaAgricultural College, Inner Mongolia Agricultural University, Hohhot 010019, P.R. ChinaAgricultural College, Inner Mongolia Agricultural University, Hohhot 010019, P.R. ChinaAgricultural College, Inner Mongolia Agricultural University, Hohhot 010019, P.R. ChinaAgricultural College, Inner Mongolia Agricultural University, Hohhot 010019, P.R. ChinaAgricultural College, Inner Mongolia Agricultural University, Hohhot 010019, P.R. ChinaAgricultural College, Inner Mongolia Agricultural University, Hohhot 010019, P.R. ChinaTo speed up the degradation of corn stover directly returned to soil at low temperature, the corn stover-degrading microbial consortium GF-20, acclimated to biological decomposition in the frigid region, was successfully constructed under a long-term limiting substrate. To evaluate its potential in accelerating the decomposition of un-pretreated corn stover, the decomposing property, fermentation dynamic and the microbial diversity were analyzed. GF-20 degraded corn stover by 32% after 15-day fermentation at 10°C. Peak activities of filter paperlyase (FPA), β-glucosidases (CB), endoglucanases (Cx), and cellobiohydrolases (C1) were 1.15, 1.67, 1.73, and 1.42 U mL−1, appearing at the 6th, 3rd, 11th, and 9th d, respectively. The pH averaged at 6.73–8.42, and the optical density (OD) value peaked at 1.87 at the 120 h of the degradation process. Cellulase, hemicellulase and lignin in corn stover were persistently degraded by 44.85, 43.85 and 25.29% at the end of incubation. Result of denaturing gradient gel electrophoresis (DGGE) profiles demonstrated that GF-20 had a stable component structure under switching the temperature and pH. The composition of the GF-20 was also analyzed by constructing bacterial 16S rDNA clone library and fungal 18SrDNA-PCR-DGGE. Twenty-two bacterial clones and four fungal bands were detected and identified dominant bacteria represented by Cellvibrio mixtus subsp., Azospira oryzae, Arcobacter defluyii, and Clostridium populeti and the fungi were mainly identified as related to Trichosporon sp.http://www.sciencedirect.com/science/article/pii/S2095311915612722corn stoverdegradationmicrobial consortiumlow temperature |
| spellingShingle | Qinggeer Ju-lin GAO Xiao-fang YU Bao-lin ZHANG Zhi-gang WANG Borjigin Naoganchaolu Shu-ping HU Ji-ying SUN Min XIE Zhen WANG Screening of a microbial consortium with efficient corn stover degradation ability at low temperature Journal of Integrative Agriculture corn stover degradation microbial consortium low temperature |
| title | Screening of a microbial consortium with efficient corn stover degradation ability at low temperature |
| title_full | Screening of a microbial consortium with efficient corn stover degradation ability at low temperature |
| title_fullStr | Screening of a microbial consortium with efficient corn stover degradation ability at low temperature |
| title_full_unstemmed | Screening of a microbial consortium with efficient corn stover degradation ability at low temperature |
| title_short | Screening of a microbial consortium with efficient corn stover degradation ability at low temperature |
| title_sort | screening of a microbial consortium with efficient corn stover degradation ability at low temperature |
| topic | corn stover degradation microbial consortium low temperature |
| url | http://www.sciencedirect.com/science/article/pii/S2095311915612722 |
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