Isolation of segmented filamentous bacteria from complex gut microbiota
Segmented filamentous bacteria (SFB) modulate the ontogeny of the immune system, and their presence can significantly affect mouse models of disease. Until recently, the inability to successfully culture SFB has made controlled studies on the mechanisms by which these bacteria exert their influence...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2015-08-01
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| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/000114319 |
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| _version_ | 1850151923805585408 |
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| author | Aaron C. Ericsson Giedre Turner Lisa Montoya Annie Wolfe Stacey Meeker Charlie Hsu Lillian Maggio-Price Craig L. Franklin |
| author_facet | Aaron C. Ericsson Giedre Turner Lisa Montoya Annie Wolfe Stacey Meeker Charlie Hsu Lillian Maggio-Price Craig L. Franklin |
| author_sort | Aaron C. Ericsson |
| collection | DOAJ |
| description | Segmented filamentous bacteria (SFB) modulate the ontogeny of the immune system, and their presence can significantly affect mouse models of disease. Until recently, the inability to successfully culture SFB has made controlled studies on the mechanisms by which these bacteria exert their influence problematic. Here, we report a new method for selecting SFB from complex microbial mixtures, providing researchers a simple and cost-effective means to prepare pure infective inocula for prospective studies and also to compare individual SFB isolates. |
| format | Article |
| id | doaj-art-e13ec98f15a3493fb91aebff13879e5b |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2015-08-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-e13ec98f15a3493fb91aebff13879e5b2025-08-20T02:26:06ZengTaylor & Francis GroupBioTechniques0736-62051940-98182015-08-01592949810.2144/000114319Isolation of segmented filamentous bacteria from complex gut microbiotaAaron C. Ericsson0Giedre Turner1Lisa Montoya2Annie Wolfe3Stacey Meeker4Charlie Hsu5Lillian Maggio-Price6Craig L. Franklin71University of Missouri (MU) Mutant Mouse Resource and Research Center, Columbia, MO1University of Missouri (MU) Mutant Mouse Resource and Research Center, Columbia, MO4Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, MO4Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, MO5Department of Comparative Medicine, School of Medicine, University of Washington, Seattle, WA5Department of Comparative Medicine, School of Medicine, University of Washington, Seattle, WA5Department of Comparative Medicine, School of Medicine, University of Washington, Seattle, WA1University of Missouri (MU) Mutant Mouse Resource and Research Center, Columbia, MOSegmented filamentous bacteria (SFB) modulate the ontogeny of the immune system, and their presence can significantly affect mouse models of disease. Until recently, the inability to successfully culture SFB has made controlled studies on the mechanisms by which these bacteria exert their influence problematic. Here, we report a new method for selecting SFB from complex microbial mixtures, providing researchers a simple and cost-effective means to prepare pure infective inocula for prospective studies and also to compare individual SFB isolates.https://www.future-science.com/doi/10.2144/000114319segmented filamentous bacteriaSFBCandidatus Arthromitusculturemicrobiotacommensal |
| spellingShingle | Aaron C. Ericsson Giedre Turner Lisa Montoya Annie Wolfe Stacey Meeker Charlie Hsu Lillian Maggio-Price Craig L. Franklin Isolation of segmented filamentous bacteria from complex gut microbiota BioTechniques segmented filamentous bacteria SFB Candidatus Arthromitus culture microbiota commensal |
| title | Isolation of segmented filamentous bacteria from complex gut microbiota |
| title_full | Isolation of segmented filamentous bacteria from complex gut microbiota |
| title_fullStr | Isolation of segmented filamentous bacteria from complex gut microbiota |
| title_full_unstemmed | Isolation of segmented filamentous bacteria from complex gut microbiota |
| title_short | Isolation of segmented filamentous bacteria from complex gut microbiota |
| title_sort | isolation of segmented filamentous bacteria from complex gut microbiota |
| topic | segmented filamentous bacteria SFB Candidatus Arthromitus culture microbiota commensal |
| url | https://www.future-science.com/doi/10.2144/000114319 |
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