CREB Phosphorylation Coincides with Transient Synapse Formation in the Rat Hippocampal Dentate Gyrus Following Avoidance Learning
Spine density change in the hippocampal dentate gyrus accompanies memory consolidation and coincides with the increased expression of ribosome-rich, hyperchromatic granule cells. Although this suggests increased protein synthesis to be required for synaptic growth in the 5 to 7 h post-training perio...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2000-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/NP.2000.279 |
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| _version_ | 1849467931359969280 |
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| author | Cormac O'Connell Helen C. Gallagher Aoibheinn O'Malley Mary Bourke Ciaran M. Regan |
| author_facet | Cormac O'Connell Helen C. Gallagher Aoibheinn O'Malley Mary Bourke Ciaran M. Regan |
| author_sort | Cormac O'Connell |
| collection | DOAJ |
| description | Spine density change in the hippocampal
dentate gyrus accompanies memory consolidation
and coincides with the increased expression of
ribosome-rich, hyperchromatic granule cells.
Although this suggests increased protein synthesis
to be required for synaptic growth in the 5 to 7 h
post-training period, little temporal mapping of
the associated molecular mechanisms has been
done. Here, we demonstrate a similar frequency
of hyperchromatic cells in naïve animals and in
those sacrificed 6 h post-training, suggesting a
transient repression of protein synthesis in the
early post-training period. Immunoblot analysis
of CREB phosphorylation in the dentate gyrus
supported this view, with downregulation from
basal levels observed at 2 to 3 h and at 12 h posttraining.
Protein synthesis reactivation appears to
be specific for de novo spine production as no
change in spine frequency accompanies the
immediate post-training period of depressed
protein synthesis. These findings support the view
that CREB-mediated gene transcription is a
requirement for long-term memory consolidation
and may be directly implicated in the process of
synaptic growth. |
| format | Article |
| id | doaj-art-e13d926c595a4da9b73b82f0bada644d |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2000-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-e13d926c595a4da9b73b82f0bada644d2025-08-20T03:25:59ZengWileyNeural Plasticity2090-59041687-54432000-01-017427928910.1155/NP.2000.279CREB Phosphorylation Coincides with Transient Synapse Formation in the Rat Hippocampal Dentate Gyrus Following Avoidance LearningCormac O'Connell0Helen C. Gallagher1Aoibheinn O'Malley2Mary Bourke3Ciaran M. Regan4Department of Pharmacology, Conway Institute, University College, Belfield, Dublin 4, IrelandDepartment of Pharmacology, Conway Institute, University College, Belfield, Dublin 4, IrelandDepartment of Pharmacology, Conway Institute, University College, Belfield, Dublin 4, IrelandDepartment of Pharmacology, Conway Institute, University College, Belfield, Dublin 4, IrelandDepartment of Pharmacology, Conway Institute, University College, Belfield, Dublin 4, IrelandSpine density change in the hippocampal dentate gyrus accompanies memory consolidation and coincides with the increased expression of ribosome-rich, hyperchromatic granule cells. Although this suggests increased protein synthesis to be required for synaptic growth in the 5 to 7 h post-training period, little temporal mapping of the associated molecular mechanisms has been done. Here, we demonstrate a similar frequency of hyperchromatic cells in naïve animals and in those sacrificed 6 h post-training, suggesting a transient repression of protein synthesis in the early post-training period. Immunoblot analysis of CREB phosphorylation in the dentate gyrus supported this view, with downregulation from basal levels observed at 2 to 3 h and at 12 h posttraining. Protein synthesis reactivation appears to be specific for de novo spine production as no change in spine frequency accompanies the immediate post-training period of depressed protein synthesis. These findings support the view that CREB-mediated gene transcription is a requirement for long-term memory consolidation and may be directly implicated in the process of synaptic growth.http://dx.doi.org/10.1155/NP.2000.279 |
| spellingShingle | Cormac O'Connell Helen C. Gallagher Aoibheinn O'Malley Mary Bourke Ciaran M. Regan CREB Phosphorylation Coincides with Transient Synapse Formation in the Rat Hippocampal Dentate Gyrus Following Avoidance Learning Neural Plasticity |
| title | CREB Phosphorylation Coincides with Transient Synapse Formation in the Rat Hippocampal Dentate Gyrus Following Avoidance Learning |
| title_full | CREB Phosphorylation Coincides with Transient Synapse Formation in the Rat Hippocampal Dentate Gyrus Following Avoidance Learning |
| title_fullStr | CREB Phosphorylation Coincides with Transient Synapse Formation in the Rat Hippocampal Dentate Gyrus Following Avoidance Learning |
| title_full_unstemmed | CREB Phosphorylation Coincides with Transient Synapse Formation in the Rat Hippocampal Dentate Gyrus Following Avoidance Learning |
| title_short | CREB Phosphorylation Coincides with Transient Synapse Formation in the Rat Hippocampal Dentate Gyrus Following Avoidance Learning |
| title_sort | creb phosphorylation coincides with transient synapse formation in the rat hippocampal dentate gyrus following avoidance learning |
| url | http://dx.doi.org/10.1155/NP.2000.279 |
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