Reduced-Pressure Boiling Extraction of Oleuropein Coupled with Ultrasonication from Olive Leaves (Olea europaea L.)

Oleuropein was extracted from Frantoio olive leaves using reduced-pressure boiling extraction coupled with ultrasound-assist (URPE). Four important factors, extraction temperature, ultrasonic power, ethanol concentration, and the ratio of solid to liquid, were selected to carry out the response surf...

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Main Authors: Pu-jun Xie, Li-xin Huang, Cai-hong Zhang, Feng You, Cheng-zhang Wang, Hao Zhou
Format: Article
Language:English
Published: Wiley 2015-01-01
Series:Advances in Materials Science and Engineering
Online Access:http://dx.doi.org/10.1155/2015/719485
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author Pu-jun Xie
Li-xin Huang
Cai-hong Zhang
Feng You
Cheng-zhang Wang
Hao Zhou
author_facet Pu-jun Xie
Li-xin Huang
Cai-hong Zhang
Feng You
Cheng-zhang Wang
Hao Zhou
author_sort Pu-jun Xie
collection DOAJ
description Oleuropein was extracted from Frantoio olive leaves using reduced-pressure boiling extraction coupled with ultrasound-assist (URPE). Four important factors, extraction temperature, ultrasonic power, ethanol concentration, and the ratio of solid to liquid, were selected to carry out the response surface methodology (RSM) for seeking optimal conditions on high oleuropein extraction yield at different levels. Box-Behnken design was employed to investigate the effects of the four factors on it. The results showed that the ratio of solid to liquid was the most significant factor of all on oleuropein yield. The optimal operation conditions were obtained as follows: ethanol concentration 75% (v/v), extraction temperature 53°C, ultrasonic power 600 W, and the ratio of solid to liquid 1 : 31. Under these optimal conditions, oleuropein extraction yield was 7.08%, which was close to the predicted value 7.121%. The scanning electron microscope (SEM) images of olive leaves after extraction were provided as well. It was seen that, compared with the untreated leaves, URPE could effectively break cells within the olive leaves.
format Article
id doaj-art-e13b9e0be4384c489ea1a26d0ad838ea
institution Kabale University
issn 1687-8434
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language English
publishDate 2015-01-01
publisher Wiley
record_format Article
series Advances in Materials Science and Engineering
spelling doaj-art-e13b9e0be4384c489ea1a26d0ad838ea2025-08-20T03:25:59ZengWileyAdvances in Materials Science and Engineering1687-84341687-84422015-01-01201510.1155/2015/719485719485Reduced-Pressure Boiling Extraction of Oleuropein Coupled with Ultrasonication from Olive Leaves (Olea europaea L.)Pu-jun Xie0Li-xin Huang1Cai-hong Zhang2Feng You3Cheng-zhang Wang4Hao Zhou5Institute of Chemical Industry of Forest Products, CAF, Nanjing, Jiangsu 210042, ChinaInstitute of Chemical Industry of Forest Products, CAF, Nanjing, Jiangsu 210042, ChinaInstitute of Chemical Industry of Forest Products, CAF, Nanjing, Jiangsu 210042, ChinaInstitute of Chemical Industry of Forest Products, CAF, Nanjing, Jiangsu 210042, ChinaInstitute of Chemical Industry of Forest Products, CAF, Nanjing, Jiangsu 210042, ChinaInstitute of Chemical Industry of Forest Products, CAF, Nanjing, Jiangsu 210042, ChinaOleuropein was extracted from Frantoio olive leaves using reduced-pressure boiling extraction coupled with ultrasound-assist (URPE). Four important factors, extraction temperature, ultrasonic power, ethanol concentration, and the ratio of solid to liquid, were selected to carry out the response surface methodology (RSM) for seeking optimal conditions on high oleuropein extraction yield at different levels. Box-Behnken design was employed to investigate the effects of the four factors on it. The results showed that the ratio of solid to liquid was the most significant factor of all on oleuropein yield. The optimal operation conditions were obtained as follows: ethanol concentration 75% (v/v), extraction temperature 53°C, ultrasonic power 600 W, and the ratio of solid to liquid 1 : 31. Under these optimal conditions, oleuropein extraction yield was 7.08%, which was close to the predicted value 7.121%. The scanning electron microscope (SEM) images of olive leaves after extraction were provided as well. It was seen that, compared with the untreated leaves, URPE could effectively break cells within the olive leaves.http://dx.doi.org/10.1155/2015/719485
spellingShingle Pu-jun Xie
Li-xin Huang
Cai-hong Zhang
Feng You
Cheng-zhang Wang
Hao Zhou
Reduced-Pressure Boiling Extraction of Oleuropein Coupled with Ultrasonication from Olive Leaves (Olea europaea L.)
Advances in Materials Science and Engineering
title Reduced-Pressure Boiling Extraction of Oleuropein Coupled with Ultrasonication from Olive Leaves (Olea europaea L.)
title_full Reduced-Pressure Boiling Extraction of Oleuropein Coupled with Ultrasonication from Olive Leaves (Olea europaea L.)
title_fullStr Reduced-Pressure Boiling Extraction of Oleuropein Coupled with Ultrasonication from Olive Leaves (Olea europaea L.)
title_full_unstemmed Reduced-Pressure Boiling Extraction of Oleuropein Coupled with Ultrasonication from Olive Leaves (Olea europaea L.)
title_short Reduced-Pressure Boiling Extraction of Oleuropein Coupled with Ultrasonication from Olive Leaves (Olea europaea L.)
title_sort reduced pressure boiling extraction of oleuropein coupled with ultrasonication from olive leaves olea europaea l
url http://dx.doi.org/10.1155/2015/719485
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