Genetic epidemiology of Plasmid Mediated Class C Beta-Lactamase among Enterobacteriaceae isolates
Introduction: Plasmid-mediated class C ß-lactamase (pAmpC) is a member of broad-spectrum ß-lactamase that spreads worldwide. However, its prevalence was under-evaluated. Objective: To characterize the prevalence and distribution of pAmpC types in 294 cefoxitin (FOX) and third-generation cephalospor...
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| Format: | Article |
| Language: | Spanish |
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Editorial Ciencias Médicas - ECIMED
2024-05-01
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| Series: | Revista Cubana de Medicina Militar |
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| Online Access: | https://revmedmilitar.sld.cu/index.php/mil/article/view/38119 |
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| author | Chuong Van Le Nguyen Nhat Tran Phuong Thi Be Nguyen Nga Thi Le Duong Thuy Le |
| author_facet | Chuong Van Le Nguyen Nhat Tran Phuong Thi Be Nguyen Nga Thi Le Duong Thuy Le |
| author_sort | Chuong Van Le |
| collection | DOAJ |
| description | Introduction: Plasmid-mediated class C ß-lactamase (pAmpC) is a member of broad-spectrum ß-lactamase that spreads worldwide. However, its prevalence was under-evaluated.
Objective: To characterize the prevalence and distribution of pAmpC types in 294 cefoxitin (FOX) and third-generation cephalosporin (3GC) resistant Enterobacteriaceae collected in several regions of Thailand and Vietnam in 2018 and 2020.
Methods: Multiplex Polymerase Chain Reaction (PCR) for pAmpC identification was utilized to investigate prevalence and diversification of pAmpC among 294 cefoxitin and third-generation cephalosporin resistant Enterobacteriaceae isolated from Thailand (n= 197) and Vietnam (n= 97).
Results: The prevalence of pAmpC was 37.1 % found in second and third-generation cephalosporin resistant Enterobacteriaceae. CMY-2 like was prominent in Thailand and Vietnam; however, prevalence of CMY-2 was varied in each hospital. DHA contributed 25.7 %, ACT/MIR rate was dominant in Chiang Rai hospital, reached 100 % in Thanh Hoa Pediatrics hospital. Worrisome, 3.7 % - isolates carried two types of pAmpC. The incidence of pAmpC in Vietnam was significantly higher than those in Thailand.
Conclusions: These findings provide evidence-based of highly spreading and diversified distribution of transferable AmpC among Enterobacteriaceae in two Asia-Pacific countries.
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| format | Article |
| id | doaj-art-e139be4eead249e6a20f6e269e0a0044 |
| institution | Kabale University |
| issn | 1561-3046 |
| language | Spanish |
| publishDate | 2024-05-01 |
| publisher | Editorial Ciencias Médicas - ECIMED |
| record_format | Article |
| series | Revista Cubana de Medicina Militar |
| spelling | doaj-art-e139be4eead249e6a20f6e269e0a00442025-08-20T03:25:11ZspaEditorial Ciencias Médicas - ECIMEDRevista Cubana de Medicina Militar1561-30462024-05-01532980Genetic epidemiology of Plasmid Mediated Class C Beta-Lactamase among Enterobacteriaceae isolatesChuong Van Le0https://orcid.org/0000-0002-7648-5716Nguyen Nhat Tran1https://orcid.org/0000-0002-5914-6659Phuong Thi Be Nguyen2https://orcid.org/0009-0002-7455-8886Nga Thi Le3https://orcid.org/0009-0005-2584-2073Duong Thuy Le4https://orcid.org/0009-0007-2066-7733University of Medicine and Pharmacy at Ho Chi Minh City. Ho Chi Minh City, VietnamUniversity of Medicine and Pharmacy at Ho Chi Minh City. Ho Chi Minh City, VietnamUniversity of Medicine and Pharmacy at Ho Chi Minh City. Ho Chi Minh City, VietnamInstitute of Public Health, Ho Chi Minh City, Viet NamMilitary Hospital 175, Ho Chi Minh City, VietnamIntroduction: Plasmid-mediated class C ß-lactamase (pAmpC) is a member of broad-spectrum ß-lactamase that spreads worldwide. However, its prevalence was under-evaluated. Objective: To characterize the prevalence and distribution of pAmpC types in 294 cefoxitin (FOX) and third-generation cephalosporin (3GC) resistant Enterobacteriaceae collected in several regions of Thailand and Vietnam in 2018 and 2020. Methods: Multiplex Polymerase Chain Reaction (PCR) for pAmpC identification was utilized to investigate prevalence and diversification of pAmpC among 294 cefoxitin and third-generation cephalosporin resistant Enterobacteriaceae isolated from Thailand (n= 197) and Vietnam (n= 97). Results: The prevalence of pAmpC was 37.1 % found in second and third-generation cephalosporin resistant Enterobacteriaceae. CMY-2 like was prominent in Thailand and Vietnam; however, prevalence of CMY-2 was varied in each hospital. DHA contributed 25.7 %, ACT/MIR rate was dominant in Chiang Rai hospital, reached 100 % in Thanh Hoa Pediatrics hospital. Worrisome, 3.7 % - isolates carried two types of pAmpC. The incidence of pAmpC in Vietnam was significantly higher than those in Thailand. Conclusions: These findings provide evidence-based of highly spreading and diversified distribution of transferable AmpC among Enterobacteriaceae in two Asia-Pacific countries. https://revmedmilitar.sld.cu/index.php/mil/article/view/38119beta-Lactamasescephalosporin resistanceenterobacteriaceaegenetic epidemiology. |
| spellingShingle | Chuong Van Le Nguyen Nhat Tran Phuong Thi Be Nguyen Nga Thi Le Duong Thuy Le Genetic epidemiology of Plasmid Mediated Class C Beta-Lactamase among Enterobacteriaceae isolates Revista Cubana de Medicina Militar beta-Lactamases cephalosporin resistance enterobacteriaceae genetic epidemiology. |
| title | Genetic epidemiology of Plasmid Mediated Class C Beta-Lactamase among Enterobacteriaceae isolates |
| title_full | Genetic epidemiology of Plasmid Mediated Class C Beta-Lactamase among Enterobacteriaceae isolates |
| title_fullStr | Genetic epidemiology of Plasmid Mediated Class C Beta-Lactamase among Enterobacteriaceae isolates |
| title_full_unstemmed | Genetic epidemiology of Plasmid Mediated Class C Beta-Lactamase among Enterobacteriaceae isolates |
| title_short | Genetic epidemiology of Plasmid Mediated Class C Beta-Lactamase among Enterobacteriaceae isolates |
| title_sort | genetic epidemiology of plasmid mediated class c beta lactamase among enterobacteriaceae isolates |
| topic | beta-Lactamases cephalosporin resistance enterobacteriaceae genetic epidemiology. |
| url | https://revmedmilitar.sld.cu/index.php/mil/article/view/38119 |
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