Characterization of nuclear microsatellites in Marchantia polymorpha (liverwort) with additional trans-specific analyses
Microsatellites are present in mitochondria, chloroplast, and nuclear DNA, but nuclear microsatellites are more useful genetic tools than those in plastids or mitochondria. Plastid and mitochondrial microsatellites have been identified in the model plant Marchantia polymorpha (liverwort), but no lab...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-11-01
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| Series: | BioTechniques |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/07366205.2024.2445454 |
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| author | Nicole Rodriguez Ortiz Niharika Sharma Tian-Xiong Zheng James J. Campanella |
| author_facet | Nicole Rodriguez Ortiz Niharika Sharma Tian-Xiong Zheng James J. Campanella |
| author_sort | Nicole Rodriguez Ortiz |
| collection | DOAJ |
| description | Microsatellites are present in mitochondria, chloroplast, and nuclear DNA, but nuclear microsatellites are more useful genetic tools than those in plastids or mitochondria. Plastid and mitochondrial microsatellites have been identified in the model plant Marchantia polymorpha (liverwort), but no laboratory has published information on nuclear microsatellite loci. The aim of this study was to detect novel nuclear markers in the most commonly employed liverwort species, design PCR primers that would allow amplification, and characterize the subsequently generated loci. We detected 18 polymorphic nuclear loci in M. polymorpha, amplifiable by PCR across all chromosomes. Additionally, trans-specific amplification of the eighteen loci was characterized in the closely related taxa Marchantia emarginata and Marchantia paleacea. All loci were present in M. paleacea, whereas 17 of 18 primer pairs were amplified in M. emarginata. |
| format | Article |
| id | doaj-art-e1397a2921ed425a90d235f56bc26d74 |
| institution | Kabale University |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-e1397a2921ed425a90d235f56bc26d742025-01-21T13:10:55ZengTaylor & Francis GroupBioTechniques0736-62051940-98182024-11-01761152753410.1080/07366205.2024.2445454Characterization of nuclear microsatellites in Marchantia polymorpha (liverwort) with additional trans-specific analysesNicole Rodriguez Ortiz0Niharika Sharma1Tian-Xiong Zheng2James J. Campanella3Department of Biology, Montclair State University, Montclair, NJ, USANSW Department of Primary Industries, Orange Agricultural Institute, Orange, AustraliaHattori Botanical Laboratory, Nichinan, JapanDepartment of Biology, Montclair State University, Montclair, NJ, USAMicrosatellites are present in mitochondria, chloroplast, and nuclear DNA, but nuclear microsatellites are more useful genetic tools than those in plastids or mitochondria. Plastid and mitochondrial microsatellites have been identified in the model plant Marchantia polymorpha (liverwort), but no laboratory has published information on nuclear microsatellite loci. The aim of this study was to detect novel nuclear markers in the most commonly employed liverwort species, design PCR primers that would allow amplification, and characterize the subsequently generated loci. We detected 18 polymorphic nuclear loci in M. polymorpha, amplifiable by PCR across all chromosomes. Additionally, trans-specific amplification of the eighteen loci was characterized in the closely related taxa Marchantia emarginata and Marchantia paleacea. All loci were present in M. paleacea, whereas 17 of 18 primer pairs were amplified in M. emarginata.https://www.tandfonline.com/doi/10.1080/07366205.2024.2445454Nuclear microsatellitesbryophyteliverwortMarchantia emarginataMarchantia paleaceaMarchantia polymorpha |
| spellingShingle | Nicole Rodriguez Ortiz Niharika Sharma Tian-Xiong Zheng James J. Campanella Characterization of nuclear microsatellites in Marchantia polymorpha (liverwort) with additional trans-specific analyses BioTechniques Nuclear microsatellites bryophyte liverwort Marchantia emarginata Marchantia paleacea Marchantia polymorpha |
| title | Characterization of nuclear microsatellites in Marchantia polymorpha (liverwort) with additional trans-specific analyses |
| title_full | Characterization of nuclear microsatellites in Marchantia polymorpha (liverwort) with additional trans-specific analyses |
| title_fullStr | Characterization of nuclear microsatellites in Marchantia polymorpha (liverwort) with additional trans-specific analyses |
| title_full_unstemmed | Characterization of nuclear microsatellites in Marchantia polymorpha (liverwort) with additional trans-specific analyses |
| title_short | Characterization of nuclear microsatellites in Marchantia polymorpha (liverwort) with additional trans-specific analyses |
| title_sort | characterization of nuclear microsatellites in marchantia polymorpha liverwort with additional trans specific analyses |
| topic | Nuclear microsatellites bryophyte liverwort Marchantia emarginata Marchantia paleacea Marchantia polymorpha |
| url | https://www.tandfonline.com/doi/10.1080/07366205.2024.2445454 |
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