Comparative genomics of multi-drug resistant Escherichia coli ST131 from human, animal, and environmental sources in the Czech Republic
Globally, Escherichia coli is a leading cause of multiple drug-resistant (MDR) extraintestinal infections. ST131 is an MDR, pandemic sequence type that causes urinary tract and bloodstream infections in humans and companion animals, and systemic infections affecting multiple organs in poultry. ST131...
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Elsevier
2025-07-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325006566 |
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| author | Michaela Ruzickova Ivana Karola Tomas Nohejl Iva Sukkar Jana Palkovicova Ivo Papousek Max L. Cummins Steven P. Djordjevic Monika Dolejska |
| author_facet | Michaela Ruzickova Ivana Karola Tomas Nohejl Iva Sukkar Jana Palkovicova Ivo Papousek Max L. Cummins Steven P. Djordjevic Monika Dolejska |
| author_sort | Michaela Ruzickova |
| collection | DOAJ |
| description | Globally, Escherichia coli is a leading cause of multiple drug-resistant (MDR) extraintestinal infections. ST131 is an MDR, pandemic sequence type that causes urinary tract and bloodstream infections in humans and companion animals, and systemic infections affecting multiple organs in poultry. ST131 has also been isolated from wastewater treatment plants and diverse wildlife sources. Studies investigating the phylogeny of ST131 from a One Health perspective are, however, limited. We performed whole-genome sequencing on 898 ST131 isolates from humans (n = 713), diverse environments (n = 139), wildlife (n = 32), food animals (n = 13), and companion animals (n = 1), sourced from the Czech Republic from 2005–2021, to identify evidence of clonal spread across different sources and to investigate carriage of genes encoding extended-spectrum beta-lactamases (ESBLs). ST131 belonging to clades C1 (262/898; 29 %) and C2 (528/898; 58.8 %) were dominant in our study. Irrespective of source or clade designation, most isolates (72.4 %) carried a blaCTX-M allele, with those residing in clade C1 carrying blaCTX-M-27, while those in clade C2 carried blaCTX-M-15. IncF plasmid sequence types were also segregated by clades, with F29:A-:B10 residing in clade A, F1:A2:B20 in clade C1, and F2:A1:B- and F31/F36:A4:B1 partitioning to clade C2. Clonal ST131 isolates (0–10 single-nucleotide polymorphisms; SNPs) were identified from humans, wild birds, and wastewater. In summary, our study showed that clonal ST131 isolates can be recovered across diverse sources in the Czech Republic, underscoring the importance of studying pandemic ExPEC lineages within a One Health context and across widespread geographic locations. |
| format | Article |
| id | doaj-art-e13885fb9e694c78ac01b9b4cacc7d34 |
| institution | OA Journals |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-e13885fb9e694c78ac01b9b4cacc7d342025-08-20T02:03:25ZengElsevierEcotoxicology and Environmental Safety0147-65132025-07-0129911832010.1016/j.ecoenv.2025.118320Comparative genomics of multi-drug resistant Escherichia coli ST131 from human, animal, and environmental sources in the Czech RepublicMichaela Ruzickova0Ivana Karola1Tomas Nohejl2Iva Sukkar3Jana Palkovicova4Ivo Papousek5Max L. Cummins6Steven P. Djordjevic7Monika Dolejska8Central European Institute of Technology, University of Veterinary Sciences Brno, Brno, Czech Republic; Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic; Department of Microbiology, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech RepublicCentral European Institute of Technology, University of Veterinary Sciences Brno, Brno, Czech RepublicCentral European Institute of Technology, University of Veterinary Sciences Brno, Brno, Czech Republic; Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech RepublicCentral European Institute of Technology, University of Veterinary Sciences Brno, Brno, Czech Republic; Department of Microbiology, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech RepublicCentral European Institute of Technology, University of Veterinary Sciences Brno, Brno, Czech Republic; Department of Microbiology, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech RepublicDepartment of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech RepublicAustralian Institute for Microbiology and Infection, University of Technology Sydney, Australia; The Australian Centre for Genomic Epidemiological Microbiology, University of Technology Sydney, AustraliaAustralian Institute for Microbiology and Infection, University of Technology Sydney, Australia; The Australian Centre for Genomic Epidemiological Microbiology, University of Technology Sydney, AustraliaCentral European Institute of Technology, University of Veterinary Sciences Brno, Brno, Czech Republic; Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czech Republic; Department of Microbiology, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech Republic; Division of Clinical Microbiology and Immunology, Department of Laboratory Medicine, University Hospital Brno, Brno, Czech Republic; Department of Chemistry and Biochemistry, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech Republic; Corresponding author at: Central European Institute of Technology, University of Veterinary Sciences Brno, Brno, Czech Republic.Globally, Escherichia coli is a leading cause of multiple drug-resistant (MDR) extraintestinal infections. ST131 is an MDR, pandemic sequence type that causes urinary tract and bloodstream infections in humans and companion animals, and systemic infections affecting multiple organs in poultry. ST131 has also been isolated from wastewater treatment plants and diverse wildlife sources. Studies investigating the phylogeny of ST131 from a One Health perspective are, however, limited. We performed whole-genome sequencing on 898 ST131 isolates from humans (n = 713), diverse environments (n = 139), wildlife (n = 32), food animals (n = 13), and companion animals (n = 1), sourced from the Czech Republic from 2005–2021, to identify evidence of clonal spread across different sources and to investigate carriage of genes encoding extended-spectrum beta-lactamases (ESBLs). ST131 belonging to clades C1 (262/898; 29 %) and C2 (528/898; 58.8 %) were dominant in our study. Irrespective of source or clade designation, most isolates (72.4 %) carried a blaCTX-M allele, with those residing in clade C1 carrying blaCTX-M-27, while those in clade C2 carried blaCTX-M-15. IncF plasmid sequence types were also segregated by clades, with F29:A-:B10 residing in clade A, F1:A2:B20 in clade C1, and F2:A1:B- and F31/F36:A4:B1 partitioning to clade C2. Clonal ST131 isolates (0–10 single-nucleotide polymorphisms; SNPs) were identified from humans, wild birds, and wastewater. In summary, our study showed that clonal ST131 isolates can be recovered across diverse sources in the Czech Republic, underscoring the importance of studying pandemic ExPEC lineages within a One Health context and across widespread geographic locations.http://www.sciencedirect.com/science/article/pii/S0147651325006566Escherichia coliAntibiotic resistanceIncF plasmidsOne HealthVirulence genesPhylogeny |
| spellingShingle | Michaela Ruzickova Ivana Karola Tomas Nohejl Iva Sukkar Jana Palkovicova Ivo Papousek Max L. Cummins Steven P. Djordjevic Monika Dolejska Comparative genomics of multi-drug resistant Escherichia coli ST131 from human, animal, and environmental sources in the Czech Republic Ecotoxicology and Environmental Safety Escherichia coli Antibiotic resistance IncF plasmids One Health Virulence genes Phylogeny |
| title | Comparative genomics of multi-drug resistant Escherichia coli ST131 from human, animal, and environmental sources in the Czech Republic |
| title_full | Comparative genomics of multi-drug resistant Escherichia coli ST131 from human, animal, and environmental sources in the Czech Republic |
| title_fullStr | Comparative genomics of multi-drug resistant Escherichia coli ST131 from human, animal, and environmental sources in the Czech Republic |
| title_full_unstemmed | Comparative genomics of multi-drug resistant Escherichia coli ST131 from human, animal, and environmental sources in the Czech Republic |
| title_short | Comparative genomics of multi-drug resistant Escherichia coli ST131 from human, animal, and environmental sources in the Czech Republic |
| title_sort | comparative genomics of multi drug resistant escherichia coli st131 from human animal and environmental sources in the czech republic |
| topic | Escherichia coli Antibiotic resistance IncF plasmids One Health Virulence genes Phylogeny |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325006566 |
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