First Evidence of Cotinine in Canine Semen Reveals Tobacco Smoke Exposure

Tobacco smoke has numerous adverse effects on both human and animal health, including impaired reproductive function. Recent research has explored environmental exposure in dogs, investigating various biological matrices. However, no data are currently available on the presence of cotinine, a nicoti...

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Main Authors: Debora Groppetti, Giulia Pizzi, Elisa Giussani, Alessandro Pecile, Silvia Michela Mazzola, Valerio Bronzo, Eleonora Fusi
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Veterinary Sciences
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Online Access:https://www.mdpi.com/2306-7381/11/12/598
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author Debora Groppetti
Giulia Pizzi
Elisa Giussani
Alessandro Pecile
Silvia Michela Mazzola
Valerio Bronzo
Eleonora Fusi
author_facet Debora Groppetti
Giulia Pizzi
Elisa Giussani
Alessandro Pecile
Silvia Michela Mazzola
Valerio Bronzo
Eleonora Fusi
author_sort Debora Groppetti
collection DOAJ
description Tobacco smoke has numerous adverse effects on both human and animal health, including impaired reproductive function. Recent research has explored environmental exposure in dogs, investigating various biological matrices. However, no data are currently available on the presence of cotinine, a nicotine metabolite, in the canine ejaculate. This study aimed to evaluate the detectability of cotinine in the semen of dogs living with smoking owners. Additionally, seminal cotinine concentrations were correlated with those in serum and hair. To further examine the potential impact of smoking on canine fertility, the relationships between seminal cotinine, total sperm concentration, and antioxidant activity in plasma and semen were analyzed in exposed and non-exposed dogs. This study is the first to demonstrate the presence of cotinine in canine ejaculate and its correlation with blood and hair concentrations. While the potential toxic effect of cotinine on seminal parameters and male fertility in dogs requires further investigation, it is crucial to raise awareness among pet owners about the risks associated with domestic smoking for their animals.
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series Veterinary Sciences
spelling doaj-art-e137b877f5b644f3a6d2f9e9e0651e8c2025-08-20T02:57:01ZengMDPI AGVeterinary Sciences2306-73812024-11-01111259810.3390/vetsci11120598First Evidence of Cotinine in Canine Semen Reveals Tobacco Smoke ExposureDebora Groppetti0Giulia Pizzi1Elisa Giussani2Alessandro Pecile3Silvia Michela Mazzola4Valerio Bronzo5Eleonora Fusi6Department of Veterinary Medicine and Animal Sciences, Università degli Studi di Milano, 26900 Lodi, ItalyPrivate Practitioner, Specialist in Animal Reproduction, 20841 Carate Brianza, MB, ItalyDepartment of Veterinary Medicine and Animal Sciences, Università degli Studi di Milano, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, Università degli Studi di Milano, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, Università degli Studi di Milano, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, Università degli Studi di Milano, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, Università degli Studi di Milano, 26900 Lodi, ItalyTobacco smoke has numerous adverse effects on both human and animal health, including impaired reproductive function. Recent research has explored environmental exposure in dogs, investigating various biological matrices. However, no data are currently available on the presence of cotinine, a nicotine metabolite, in the canine ejaculate. This study aimed to evaluate the detectability of cotinine in the semen of dogs living with smoking owners. Additionally, seminal cotinine concentrations were correlated with those in serum and hair. To further examine the potential impact of smoking on canine fertility, the relationships between seminal cotinine, total sperm concentration, and antioxidant activity in plasma and semen were analyzed in exposed and non-exposed dogs. This study is the first to demonstrate the presence of cotinine in canine ejaculate and its correlation with blood and hair concentrations. While the potential toxic effect of cotinine on seminal parameters and male fertility in dogs requires further investigation, it is crucial to raise awareness among pet owners about the risks associated with domestic smoking for their animals.https://www.mdpi.com/2306-7381/11/12/598cigarettedogoxidative stresssperm concentrationsmoking
spellingShingle Debora Groppetti
Giulia Pizzi
Elisa Giussani
Alessandro Pecile
Silvia Michela Mazzola
Valerio Bronzo
Eleonora Fusi
First Evidence of Cotinine in Canine Semen Reveals Tobacco Smoke Exposure
Veterinary Sciences
cigarette
dog
oxidative stress
sperm concentration
smoking
title First Evidence of Cotinine in Canine Semen Reveals Tobacco Smoke Exposure
title_full First Evidence of Cotinine in Canine Semen Reveals Tobacco Smoke Exposure
title_fullStr First Evidence of Cotinine in Canine Semen Reveals Tobacco Smoke Exposure
title_full_unstemmed First Evidence of Cotinine in Canine Semen Reveals Tobacco Smoke Exposure
title_short First Evidence of Cotinine in Canine Semen Reveals Tobacco Smoke Exposure
title_sort first evidence of cotinine in canine semen reveals tobacco smoke exposure
topic cigarette
dog
oxidative stress
sperm concentration
smoking
url https://www.mdpi.com/2306-7381/11/12/598
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