Impaired LTB4-induced neutrophil chemotactic directionality in myelodysplastic neoplasms patients
Objectives Myelodysplastic neoplasm (MDS) patients are at a high risk of infections, contributing significantly to morbidity and mortality. While neutrophil dysfunction is considered a primary factor, specific functional defects remain elusive.Methods We conducted a comprehensive study involving 90...
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Taylor & Francis Group
2025-12-01
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| Series: | Hematology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/16078454.2025.2483551 |
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| author | Xinyan Xie Yumei Liu Liyan Yang Zhe Zhang Hongzhao Li Wei Zhang Hong Liu Huaquan Wang Zonghong Shao |
| author_facet | Xinyan Xie Yumei Liu Liyan Yang Zhe Zhang Hongzhao Li Wei Zhang Hong Liu Huaquan Wang Zonghong Shao |
| author_sort | Xinyan Xie |
| collection | DOAJ |
| description | Objectives Myelodysplastic neoplasm (MDS) patients are at a high risk of infections, contributing significantly to morbidity and mortality. While neutrophil dysfunction is considered a primary factor, specific functional defects remain elusive.Methods We conducted a comprehensive study involving 90 participants, including controls and de novo MDS patients. We utilized the TAXIScan-FL system to evaluate neutrophil chemotaxis towards leukotriene B4 (LTB4). The global reactive oxygen species (ROS) production by neutrophils were measured by chemiluminescence assay, neutrophil alkaline phosphatase (NAP) was evaluated by enzymatic staining.Results MDS patients, irrespective of absolute neutrophil count (ANC) levels, exhibited elevated empirical antimicrobial therapy (EAT) rate compared to controls. Neutrophil migration towards LTB4 was notably impaired, demonstrating reduced velocity and directionality. Interestingly, MDS patients with high ANC still displayed poor directionality and slower migration. MDS patients also had compromised ROS and NAP activity. A noteworthy correlation was observed between EAT rate and chemotactic directionality parameters.Conclusion MDS patients face a heightened risk of infection, potentially attributed to impaired neutrophil chemotactic speed and directionality, alongside compromised ROS and NAP activity. Notably, chemotactic directionality emerged as a pivotal factor correlated with antimicrobial therapy. These insights hold significant clinical implications for managing infections in MDS patients, underscoring the importance of targeting specific neutrophil defects for more effective therapeutic strategies. |
| format | Article |
| id | doaj-art-e125f1ae9926439098ce01dcc55291d6 |
| institution | OA Journals |
| issn | 1607-8454 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Hematology |
| spelling | doaj-art-e125f1ae9926439098ce01dcc55291d62025-08-20T01:55:42ZengTaylor & Francis GroupHematology1607-84542025-12-0130110.1080/16078454.2025.2483551Impaired LTB4-induced neutrophil chemotactic directionality in myelodysplastic neoplasms patientsXinyan Xie0Yumei Liu1Liyan Yang2Zhe Zhang3Hongzhao Li4Wei Zhang5Hong Liu6Huaquan Wang7Zonghong Shao8Department of Hematology, Tianjin Medical University General Hospital, Tianjin, People’s Republic of ChinaDepartment of Hematology, Tianjin Medical University General Hospital, Tianjin, People’s Republic of ChinaDepartment of Hematology, Tianjin Medical University General Hospital, Tianjin, People’s Republic of ChinaDepartment of Hematology, Tianjin Medical University General Hospital, Tianjin, People’s Republic of ChinaTianjin Children’s Hospital, Tianjin, People’s Republic of ChinaDepartment of Hematology, Tianjin Medical University General Hospital, Tianjin, People’s Republic of ChinaDepartment of Hematology, Tianjin Medical University General Hospital, Tianjin, People’s Republic of ChinaDepartment of Hematology, Tianjin Medical University General Hospital, Tianjin, People’s Republic of ChinaDepartment of Hematology, Tianjin Medical University General Hospital, Tianjin, People’s Republic of ChinaObjectives Myelodysplastic neoplasm (MDS) patients are at a high risk of infections, contributing significantly to morbidity and mortality. While neutrophil dysfunction is considered a primary factor, specific functional defects remain elusive.Methods We conducted a comprehensive study involving 90 participants, including controls and de novo MDS patients. We utilized the TAXIScan-FL system to evaluate neutrophil chemotaxis towards leukotriene B4 (LTB4). The global reactive oxygen species (ROS) production by neutrophils were measured by chemiluminescence assay, neutrophil alkaline phosphatase (NAP) was evaluated by enzymatic staining.Results MDS patients, irrespective of absolute neutrophil count (ANC) levels, exhibited elevated empirical antimicrobial therapy (EAT) rate compared to controls. Neutrophil migration towards LTB4 was notably impaired, demonstrating reduced velocity and directionality. Interestingly, MDS patients with high ANC still displayed poor directionality and slower migration. MDS patients also had compromised ROS and NAP activity. A noteworthy correlation was observed between EAT rate and chemotactic directionality parameters.Conclusion MDS patients face a heightened risk of infection, potentially attributed to impaired neutrophil chemotactic speed and directionality, alongside compromised ROS and NAP activity. Notably, chemotactic directionality emerged as a pivotal factor correlated with antimicrobial therapy. These insights hold significant clinical implications for managing infections in MDS patients, underscoring the importance of targeting specific neutrophil defects for more effective therapeutic strategies.https://www.tandfonline.com/doi/10.1080/16078454.2025.2483551Myelodysplastic neoplasmsneutrophilleukotriene B4chemotaxisTAXIScanreactive oxygen species |
| spellingShingle | Xinyan Xie Yumei Liu Liyan Yang Zhe Zhang Hongzhao Li Wei Zhang Hong Liu Huaquan Wang Zonghong Shao Impaired LTB4-induced neutrophil chemotactic directionality in myelodysplastic neoplasms patients Hematology Myelodysplastic neoplasms neutrophil leukotriene B4 chemotaxis TAXIScan reactive oxygen species |
| title | Impaired LTB4-induced neutrophil chemotactic directionality in myelodysplastic neoplasms patients |
| title_full | Impaired LTB4-induced neutrophil chemotactic directionality in myelodysplastic neoplasms patients |
| title_fullStr | Impaired LTB4-induced neutrophil chemotactic directionality in myelodysplastic neoplasms patients |
| title_full_unstemmed | Impaired LTB4-induced neutrophil chemotactic directionality in myelodysplastic neoplasms patients |
| title_short | Impaired LTB4-induced neutrophil chemotactic directionality in myelodysplastic neoplasms patients |
| title_sort | impaired ltb4 induced neutrophil chemotactic directionality in myelodysplastic neoplasms patients |
| topic | Myelodysplastic neoplasms neutrophil leukotriene B4 chemotaxis TAXIScan reactive oxygen species |
| url | https://www.tandfonline.com/doi/10.1080/16078454.2025.2483551 |
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