Characterization of total phenol and flavonoid contents, colour, functional properties from honey samples with different floral origins

Honey has long been used as a food and has been reported to have potential health benefits. In this work, total phenol content, colour and antioxidant and hepatoprotective activities of honey samples of different floral origins from the State of Hidalgo, Mexico were explored using in vitro assays. H...

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Main Authors: Alma Delia Hernández-Fuentes, David Chávez-Borges, Antonio de Jesús Cenobio-Galindo, Andrea Paloma Zepeda-Velázquez, Ana Cristina Figueira, Rubén Jiménez-Alvarado, Rafael Germán Campos-Montiel
Format: Article
Language:English
Published: ISEKI_Food Association (IFA) 2021-10-01
Series:International Journal of Food Studies
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Online Access:https://www.iseki-food-ejournal.com/article/138
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author Alma Delia Hernández-Fuentes
David Chávez-Borges
Antonio de Jesús Cenobio-Galindo
Andrea Paloma Zepeda-Velázquez
Ana Cristina Figueira
Rubén Jiménez-Alvarado
Rafael Germán Campos-Montiel
author_facet Alma Delia Hernández-Fuentes
David Chávez-Borges
Antonio de Jesús Cenobio-Galindo
Andrea Paloma Zepeda-Velázquez
Ana Cristina Figueira
Rubén Jiménez-Alvarado
Rafael Germán Campos-Montiel
author_sort Alma Delia Hernández-Fuentes
collection DOAJ
description Honey has long been used as a food and has been reported to have potential health benefits. In this work, total phenol content, colour and antioxidant and hepatoprotective activities of honey samples of different floral origins from the State of Hidalgo, Mexico were explored using in vitro assays. Hepatoprotective activity was measured by inhibitition of β-glucuronidase; gastroprotective activity was determined by inhibition of urease; antioxidant activity was evaluated by 2,2'-Azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) methods. All the parameters showed significant differences (p<0.05) among honey samples. The total phenolic content ranged from 18.02 to 102.77 mg GAE 100 g-1. The colour ranged from extra light amber to dark amber. Inhibition of β-glucuronidase ranged from 23.70% to 36.00%, while urease inhibition ranged between 7.64% and 63.80%. The antioxidant activity by ABTS was between 44.68 and 441.56 mg AAE 100 g-1, and DPPH showed activities ranging from 35.64 to 573.06 mg AAE 100 g-1. All honey samples contained bioactive compounds and displayed functional properties; therefore, the honeys from this region of Mexico offer attractive characteristics for their potential use in the food industry.
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publisher ISEKI_Food Association (IFA)
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spelling doaj-art-e120ab5a4d774fa1886b50b703abd58d2025-08-20T02:21:51ZengISEKI_Food Association (IFA)International Journal of Food Studies2182-10542021-10-0110210.7455/ijfs/10.2.2021.a6Characterization of total phenol and flavonoid contents, colour, functional properties from honey samples with different floral originsAlma Delia Hernández-Fuentes0David Chávez-Borges1Antonio de Jesús Cenobio-Galindo2Andrea Paloma Zepeda-Velázquez3Ana Cristina Figueira4Rubén Jiménez-Alvarado5Rafael Germán Campos-Montiel6Universidad Autónoma del Estado de Hidalgo, Pachuca, MexicoUniversidad Autónoma del Estado de Hidalgo, Pachuca, MexicoUniversidad Autónoma del Estado de Hidalgo, Pachuca, MexicoUniversidad Autónoma del Estado de Hidalgo, Pachuca, MexicoUniversity of the AlgarveUniversidad Autónoma del Estado de Hidalgo, Pachuca, MexicoUniversidad Autónoma del Estado de Hidalgo, Pachuca, MexicoHoney has long been used as a food and has been reported to have potential health benefits. In this work, total phenol content, colour and antioxidant and hepatoprotective activities of honey samples of different floral origins from the State of Hidalgo, Mexico were explored using in vitro assays. Hepatoprotective activity was measured by inhibitition of β-glucuronidase; gastroprotective activity was determined by inhibition of urease; antioxidant activity was evaluated by 2,2'-Azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) methods. All the parameters showed significant differences (p<0.05) among honey samples. The total phenolic content ranged from 18.02 to 102.77 mg GAE 100 g-1. The colour ranged from extra light amber to dark amber. Inhibition of β-glucuronidase ranged from 23.70% to 36.00%, while urease inhibition ranged between 7.64% and 63.80%. The antioxidant activity by ABTS was between 44.68 and 441.56 mg AAE 100 g-1, and DPPH showed activities ranging from 35.64 to 573.06 mg AAE 100 g-1. All honey samples contained bioactive compounds and displayed functional properties; therefore, the honeys from this region of Mexico offer attractive characteristics for their potential use in the food industry.https://www.iseki-food-ejournal.com/article/138PhenolsFlavonoidsColourβ-glucuronidaseUrease
spellingShingle Alma Delia Hernández-Fuentes
David Chávez-Borges
Antonio de Jesús Cenobio-Galindo
Andrea Paloma Zepeda-Velázquez
Ana Cristina Figueira
Rubén Jiménez-Alvarado
Rafael Germán Campos-Montiel
Characterization of total phenol and flavonoid contents, colour, functional properties from honey samples with different floral origins
International Journal of Food Studies
Phenols
Flavonoids
Colour
β-glucuronidase
Urease
title Characterization of total phenol and flavonoid contents, colour, functional properties from honey samples with different floral origins
title_full Characterization of total phenol and flavonoid contents, colour, functional properties from honey samples with different floral origins
title_fullStr Characterization of total phenol and flavonoid contents, colour, functional properties from honey samples with different floral origins
title_full_unstemmed Characterization of total phenol and flavonoid contents, colour, functional properties from honey samples with different floral origins
title_short Characterization of total phenol and flavonoid contents, colour, functional properties from honey samples with different floral origins
title_sort characterization of total phenol and flavonoid contents colour functional properties from honey samples with different floral origins
topic Phenols
Flavonoids
Colour
β-glucuronidase
Urease
url https://www.iseki-food-ejournal.com/article/138
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