Investigation of genetic polymorphisms in genes encoding growth factors and dental pulp calcification in orthodontic patients
Background and objectives: Pulp calcification is associated with many factors and triggers, including individual genetic predisposition and orthodontic forces. This study aimed to investigate whether genetic polymorphisms in epidermal growth factor (EGF), epidermal growth factor receptor (EGFR1), tr...
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Elsevier
2024-11-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221242682400143X |
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| author | Sandra Regina Santos Meyfarth Iago Ramirez Alice Corrêa Silva-Sousa Peter Proff Marilisa Carneiro Leão Gabardo Manoel Damião Sousa-Neto Flares Baratto-Filho Erika Calvano Küchler Leonardo Santos Antunes Christian Kirschneck |
| author_facet | Sandra Regina Santos Meyfarth Iago Ramirez Alice Corrêa Silva-Sousa Peter Proff Marilisa Carneiro Leão Gabardo Manoel Damião Sousa-Neto Flares Baratto-Filho Erika Calvano Küchler Leonardo Santos Antunes Christian Kirschneck |
| author_sort | Sandra Regina Santos Meyfarth |
| collection | DOAJ |
| description | Background and objectives: Pulp calcification is associated with many factors and triggers, including individual genetic predisposition and orthodontic forces. This study aimed to investigate whether genetic polymorphisms in epidermal growth factor (EGF), epidermal growth factor receptor (EGFR1), transforming growth factor-beta 1 (TGFβ1), and transforming growth factor-beta receptor 2 (TGFβR2) are associated with a risk of dental pulp calcifications in orthodontic patients. Materials and methods: Digital orthopantomography (OPG) and genomic DNA from 132 patients were analyzed in this cross-sectional study. Pulp calcification was observed in the maxillary and mandibular first molars. Genomic DNA extracted from saliva cells was used to genotype eight genetic polymorphisms using real-time polymerase chain reaction: EGF (rs2237051 and rs4444903), EGFR (rs2227983 and rs763317), TGFβ1 (rs1800469 and rs4803455), and TGFβR2 (rs3087465 and rs764522). The association between pulp calcification and genetic polymorphisms was analyzed using allelic and genotypic distributions, and haplotype frequencies (P < 0.05). Results: The prevalence of pulp calcification was 42.4 % in 490 molars. Genotypic analysis and allelic distribution showed no statistically significant association between the evaluated growth factors and molar calcification (P > 0.05). No haplotype combinations showed a statistically significant difference (P > 0.05). Conclusion: The genetic polymorphisms investigated were not associated with dental pulp calcification in orthodontic patients. Further studies should investigate other polymorphisms in genes encoding growth factors. |
| format | Article |
| id | doaj-art-e11a4f1671be4a2c9eb5fab70d7f0da8 |
| institution | OA Journals |
| issn | 2212-4268 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-e11a4f1671be4a2c9eb5fab70d7f0da82025-08-20T02:05:28ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682024-11-0114671271910.1016/j.jobcr.2024.09.013Investigation of genetic polymorphisms in genes encoding growth factors and dental pulp calcification in orthodontic patientsSandra Regina Santos Meyfarth0Iago Ramirez1Alice Corrêa Silva-Sousa2Peter Proff3Marilisa Carneiro Leão Gabardo4Manoel Damião Sousa-Neto5Flares Baratto-Filho6Erika Calvano Küchler7Leonardo Santos Antunes8Christian Kirschneck9Postgraduation Program, School of Dentistry, Fluminense Federal University, Niterói, RJ, BrazilSchool of Dentistry, University of São Paulo, Ribeirão Preto, SP, BrazilSchool of Dentistry, University of São Paulo, Ribeirão Preto, SP, BrazilDepartment of Orthodontics, University of Regensburg, Regensburg, GermanySchool of Dentistry, Positivo University, Curitiba, PR, BrazilSchool of Dentistry, University of São Paulo, Ribeirão Preto, SP, BrazilSchool of Dentistry, Tuiuti University from Paraná, Curitiba, PR, Brazil; Department of Dentistry, University of Joinville Region (Univille), Joinville, SC, BrazilDepartment of Orthodontics, University Hospital Bonn, Medical Faculty, Bonn, Germany; Corresponding author. Department of Orthodontics, University of Bonn, Welschnonnenstr. 17, 53111, Bonn, Germany.Postgraduation Program, School of Dentistry, Fluminense Federal University, Niterói, RJ, Brazil; Department of Specific Formation, Fluminense Federal University, Niterói, RJ, BrazilDepartment of Orthodontics, University Hospital Bonn, Medical Faculty, Bonn, GermanyBackground and objectives: Pulp calcification is associated with many factors and triggers, including individual genetic predisposition and orthodontic forces. This study aimed to investigate whether genetic polymorphisms in epidermal growth factor (EGF), epidermal growth factor receptor (EGFR1), transforming growth factor-beta 1 (TGFβ1), and transforming growth factor-beta receptor 2 (TGFβR2) are associated with a risk of dental pulp calcifications in orthodontic patients. Materials and methods: Digital orthopantomography (OPG) and genomic DNA from 132 patients were analyzed in this cross-sectional study. Pulp calcification was observed in the maxillary and mandibular first molars. Genomic DNA extracted from saliva cells was used to genotype eight genetic polymorphisms using real-time polymerase chain reaction: EGF (rs2237051 and rs4444903), EGFR (rs2227983 and rs763317), TGFβ1 (rs1800469 and rs4803455), and TGFβR2 (rs3087465 and rs764522). The association between pulp calcification and genetic polymorphisms was analyzed using allelic and genotypic distributions, and haplotype frequencies (P < 0.05). Results: The prevalence of pulp calcification was 42.4 % in 490 molars. Genotypic analysis and allelic distribution showed no statistically significant association between the evaluated growth factors and molar calcification (P > 0.05). No haplotype combinations showed a statistically significant difference (P > 0.05). Conclusion: The genetic polymorphisms investigated were not associated with dental pulp calcification in orthodontic patients. Further studies should investigate other polymorphisms in genes encoding growth factors.http://www.sciencedirect.com/science/article/pii/S221242682400143XDental pulp calcificationGenetic polymorphismEGFEGFRTGFβ1TGFβR2 |
| spellingShingle | Sandra Regina Santos Meyfarth Iago Ramirez Alice Corrêa Silva-Sousa Peter Proff Marilisa Carneiro Leão Gabardo Manoel Damião Sousa-Neto Flares Baratto-Filho Erika Calvano Küchler Leonardo Santos Antunes Christian Kirschneck Investigation of genetic polymorphisms in genes encoding growth factors and dental pulp calcification in orthodontic patients Journal of Oral Biology and Craniofacial Research Dental pulp calcification Genetic polymorphism EGF EGFR TGFβ1 TGFβR2 |
| title | Investigation of genetic polymorphisms in genes encoding growth factors and dental pulp calcification in orthodontic patients |
| title_full | Investigation of genetic polymorphisms in genes encoding growth factors and dental pulp calcification in orthodontic patients |
| title_fullStr | Investigation of genetic polymorphisms in genes encoding growth factors and dental pulp calcification in orthodontic patients |
| title_full_unstemmed | Investigation of genetic polymorphisms in genes encoding growth factors and dental pulp calcification in orthodontic patients |
| title_short | Investigation of genetic polymorphisms in genes encoding growth factors and dental pulp calcification in orthodontic patients |
| title_sort | investigation of genetic polymorphisms in genes encoding growth factors and dental pulp calcification in orthodontic patients |
| topic | Dental pulp calcification Genetic polymorphism EGF EGFR TGFβ1 TGFβR2 |
| url | http://www.sciencedirect.com/science/article/pii/S221242682400143X |
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