Optimisation of amplified fragment length polymorphism (AFLP) analysis of hop wilt (Verticillium alboatrum and Verticillium dahliae)
Hop wilt causes traheomycotic fungi Verticillium alboatrum Reinke & Berthold and Verticillium dahliae Klebahn. On hop, the disease appears in mild and lethal forms, depending on the virulence of isolates, sensitivity of cultivars and ecological factors. Identification of the disease and its path...
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2001-12-01
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| Series: | Acta Agriculturae Slovenica |
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| Online Access: | https://journals.uni-lj.si/aas/article/view/15705 |
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| author | Sebastjan RADIŠEK Jernej JAKŠE Branka JAVORNIK |
| author_facet | Sebastjan RADIŠEK Jernej JAKŠE Branka JAVORNIK |
| author_sort | Sebastjan RADIŠEK |
| collection | DOAJ |
| description | Hop wilt causes traheomycotic fungi Verticillium alboatrum Reinke & Berthold and Verticillium dahliae Klebahn. On hop, the disease appears in mild and lethal forms, depending on the virulence of isolates, sensitivity of cultivars and ecological factors. Identification of the disease and its pathotypes is important for suitable phytosanitary measures and disease resistance breeding. Methods in traditional detection and diagnosis are usually lengthy and laborious, which could be improved and supplemented by molecular methods. This paper presents a modification of the AFLP method suitable for analysis of Verticillium species. By means of such AFLP, we were able to distinguish the two Verticillium species and found polymorphisms between mild and lethal forms of Verticillium alboatrum from hop. |
| format | Article |
| id | doaj-art-e0fee775121340e8959345cdd80b324d |
| institution | DOAJ |
| issn | 1854-1941 |
| language | English |
| publishDate | 2001-12-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj-art-e0fee775121340e8959345cdd80b324d2025-08-20T03:02:26ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412001-12-0177213914610.14720/aas.2001.77.2.1570522098Optimisation of amplified fragment length polymorphism (AFLP) analysis of hop wilt (Verticillium alboatrum and Verticillium dahliae)Sebastjan RADIŠEKJernej JAKŠEBranka JAVORNIKHop wilt causes traheomycotic fungi Verticillium alboatrum Reinke & Berthold and Verticillium dahliae Klebahn. On hop, the disease appears in mild and lethal forms, depending on the virulence of isolates, sensitivity of cultivars and ecological factors. Identification of the disease and its pathotypes is important for suitable phytosanitary measures and disease resistance breeding. Methods in traditional detection and diagnosis are usually lengthy and laborious, which could be improved and supplemented by molecular methods. This paper presents a modification of the AFLP method suitable for analysis of Verticillium species. By means of such AFLP, we were able to distinguish the two Verticillium species and found polymorphisms between mild and lethal forms of Verticillium alboatrum from hop.https://journals.uni-lj.si/aas/article/view/15705verticillium alboatrumverticillium dahliaemolecular markersaflppcr |
| spellingShingle | Sebastjan RADIŠEK Jernej JAKŠE Branka JAVORNIK Optimisation of amplified fragment length polymorphism (AFLP) analysis of hop wilt (Verticillium alboatrum and Verticillium dahliae) Acta Agriculturae Slovenica verticillium alboatrum verticillium dahliae molecular markers aflp pcr |
| title | Optimisation of amplified fragment length polymorphism (AFLP) analysis of hop wilt (Verticillium alboatrum and Verticillium dahliae) |
| title_full | Optimisation of amplified fragment length polymorphism (AFLP) analysis of hop wilt (Verticillium alboatrum and Verticillium dahliae) |
| title_fullStr | Optimisation of amplified fragment length polymorphism (AFLP) analysis of hop wilt (Verticillium alboatrum and Verticillium dahliae) |
| title_full_unstemmed | Optimisation of amplified fragment length polymorphism (AFLP) analysis of hop wilt (Verticillium alboatrum and Verticillium dahliae) |
| title_short | Optimisation of amplified fragment length polymorphism (AFLP) analysis of hop wilt (Verticillium alboatrum and Verticillium dahliae) |
| title_sort | optimisation of amplified fragment length polymorphism aflp analysis of hop wilt verticillium alboatrum and verticillium dahliae |
| topic | verticillium alboatrum verticillium dahliae molecular markers aflp pcr |
| url | https://journals.uni-lj.si/aas/article/view/15705 |
| work_keys_str_mv | AT sebastjanradisek optimisationofamplifiedfragmentlengthpolymorphismaflpanalysisofhopwiltverticilliumalboatrumandverticilliumdahliae AT jernejjakse optimisationofamplifiedfragmentlengthpolymorphismaflpanalysisofhopwiltverticilliumalboatrumandverticilliumdahliae AT brankajavornik optimisationofamplifiedfragmentlengthpolymorphismaflpanalysisofhopwiltverticilliumalboatrumandverticilliumdahliae |