Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining waste
Seeds of four native species of trees and shrubs (Larrea cuneifolia, Bulnesia retama, Plectrocarpa tetracantha and Prosopis flexuosa) were exposed to soil contaminated with As, Cu, Cd, and Zn from an abandoned gold mine to identify adaptation strategies. Several physiological, morpho-anatomical, and...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325000867 |
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| author | Belén Heredia Pablo Diaz Recio Brian Jonathan Young Pablo Pacheco Franco Barcelo Zungre Silvina Hruby Maria Andreína Acevedo María Inés Mercado Gonzalo Roqueiro |
| author_facet | Belén Heredia Pablo Diaz Recio Brian Jonathan Young Pablo Pacheco Franco Barcelo Zungre Silvina Hruby Maria Andreína Acevedo María Inés Mercado Gonzalo Roqueiro |
| author_sort | Belén Heredia |
| collection | DOAJ |
| description | Seeds of four native species of trees and shrubs (Larrea cuneifolia, Bulnesia retama, Plectrocarpa tetracantha and Prosopis flexuosa) were exposed to soil contaminated with As, Cu, Cd, and Zn from an abandoned gold mine to identify adaptation strategies. Several physiological, morpho-anatomical, and biochemical parameters were determined. The seed germination of L. cuneifolia, B. retama, and P. tetracantha was fully inhibited in 100 % contaminated soil. Toxicological endpoints as NOEC, LOEC and IC50 ranged from 10 % to 25 % of soil contaminated with mining waste. Radicle elongation was the most sensitive variable to high metal(loid) concentrations, except for L. cuneifolia that hypocotyl elongation was the most affected parameter. P. flexuosa was selected to evaluate biochemical biomarkers and morpho-anatomical parameters. It showed an increase in radicle diameter and central radicle cylinder. A concentration-dependent increase in the O2·- production was observed in radicle and cotyledon. A peak of the enzymatic activity of guaiacol peroxidase, ascorbate peroxidase and catalase enzymes in P. flexuosa seedlings showed a negative relationship between metal(loid) concentration and exposure time. After a drop in the enzymatic activity, an increase in the malondialdehyde content (lipid peroxidation) was observed. The tested native species could be useful for phytoremediation of soils with a very high degree of metal contamination. A further investigation should focus on strategies to improve soil physicochemical characteristics for plant survival at highest contamination levels. |
| format | Article |
| id | doaj-art-e0fdf57dbed047eb8d9555a86d82a060 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-e0fdf57dbed047eb8d9555a86d82a0602025-02-12T05:30:11ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01290117750Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining wasteBelén Heredia0Pablo Diaz Recio1Brian Jonathan Young2Pablo Pacheco3Franco Barcelo Zungre4Silvina Hruby5Maria Andreína Acevedo6María Inés Mercado7Gonzalo Roqueiro8Universidad Nacional de San Juan, Facultad de Ingeniería (FI-UNSJ), Av. Lib. San Martín (Oeste) 1109, San Juan, San Juan 5400, Argentina; Instituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Agropecuaria San Juan, Calle 11 y Vidart, Pocito, San Juan 5427, Argentina; Correspondence to: Facultad de Ingeniería-UNSJ, Av. Libertador Gral. San Martín 1109, San Juan, San Juan 5400, Argentina.Universidad Nacional de San Juan, Facultad de Ingeniería (FI-UNSJ), Av. Lib. San Martín (Oeste) 1109, San Juan, San Juan 5400, ArgentinaInstituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Microbiología y Zoología Agrícola (IMyZA), Nicolas Repetto 2751, Hurlingham, Buenos Aires B1686, ArgentinaInstituto de Química San Luis (INQUISAL-CONICET), Chacabuco y Pedernera s/n, San Luis 5700, ArgentinaInstituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Agropecuaria San Juan, Calle 11 y Vidart, Pocito, San Juan 5427, Argentina; Consejo Nacional de Investigación Científica y Técnica (CONICET-CCT San Juan), Facultad de Ingeniería-UNSJ, Av. Libertador Gral. San Martín 1109, San Juan 5400, ArgentinaInstituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Agropecuaria San Juan, Calle 11 y Vidart, Pocito, San Juan 5427, Argentina; Consejo Nacional de Investigación Científica y Técnica (CONICET-CCT San Juan), Facultad de Ingeniería-UNSJ, Av. Libertador Gral. San Martín 1109, San Juan 5400, ArgentinaFundación Miguel Lillo, Instituto de Morfología Vegetal, Calle Miguel Lillo 251, San Miguel de Tucumán, Tucumán T4000JFE, ArgentinaFundación Miguel Lillo, Instituto de Morfología Vegetal, Calle Miguel Lillo 251, San Miguel de Tucumán, Tucumán T4000JFE, ArgentinaUniversidad Nacional de San Juan, Facultad de Ingeniería (FI-UNSJ), Av. Lib. San Martín (Oeste) 1109, San Juan, San Juan 5400, Argentina; Instituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Agropecuaria San Juan, Calle 11 y Vidart, Pocito, San Juan 5427, ArgentinaSeeds of four native species of trees and shrubs (Larrea cuneifolia, Bulnesia retama, Plectrocarpa tetracantha and Prosopis flexuosa) were exposed to soil contaminated with As, Cu, Cd, and Zn from an abandoned gold mine to identify adaptation strategies. Several physiological, morpho-anatomical, and biochemical parameters were determined. The seed germination of L. cuneifolia, B. retama, and P. tetracantha was fully inhibited in 100 % contaminated soil. Toxicological endpoints as NOEC, LOEC and IC50 ranged from 10 % to 25 % of soil contaminated with mining waste. Radicle elongation was the most sensitive variable to high metal(loid) concentrations, except for L. cuneifolia that hypocotyl elongation was the most affected parameter. P. flexuosa was selected to evaluate biochemical biomarkers and morpho-anatomical parameters. It showed an increase in radicle diameter and central radicle cylinder. A concentration-dependent increase in the O2·- production was observed in radicle and cotyledon. A peak of the enzymatic activity of guaiacol peroxidase, ascorbate peroxidase and catalase enzymes in P. flexuosa seedlings showed a negative relationship between metal(loid) concentration and exposure time. After a drop in the enzymatic activity, an increase in the malondialdehyde content (lipid peroxidation) was observed. The tested native species could be useful for phytoremediation of soils with a very high degree of metal contamination. A further investigation should focus on strategies to improve soil physicochemical characteristics for plant survival at highest contamination levels.http://www.sciencedirect.com/science/article/pii/S0147651325000867Oxidative stressMetalPhytoremediationSoil pollutionArid environmentPhytotoxicity |
| spellingShingle | Belén Heredia Pablo Diaz Recio Brian Jonathan Young Pablo Pacheco Franco Barcelo Zungre Silvina Hruby Maria Andreína Acevedo María Inés Mercado Gonzalo Roqueiro Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining waste Ecotoxicology and Environmental Safety Oxidative stress Metal Phytoremediation Soil pollution Arid environment Phytotoxicity |
| title | Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining waste |
| title_full | Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining waste |
| title_fullStr | Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining waste |
| title_full_unstemmed | Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining waste |
| title_short | Strategies of physiological, morpho-anatomical and biochemical adaptation in seedlings of native species exposed to mining waste |
| title_sort | strategies of physiological morpho anatomical and biochemical adaptation in seedlings of native species exposed to mining waste |
| topic | Oxidative stress Metal Phytoremediation Soil pollution Arid environment Phytotoxicity |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325000867 |
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