Hesperetin Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication by Downregulating the P38/JUN/FOS Pathway In Vitro
Porcine reproductive and respiratory syndrome virus (PRRSV) is a persistent pathogen that causes significant economic losses to the global swine industry. Commercial vaccines provide only partial protection, and no effective therapeutic treatments are currently available. In this study, we demonstra...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/13/2/450 |
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| author | Ruiheng Gu Feike Zhao Quanying Li Limin Hou Guochang Liu Xueyan Sun Junyuan Du Binghu Fang |
| author_facet | Ruiheng Gu Feike Zhao Quanying Li Limin Hou Guochang Liu Xueyan Sun Junyuan Du Binghu Fang |
| author_sort | Ruiheng Gu |
| collection | DOAJ |
| description | Porcine reproductive and respiratory syndrome virus (PRRSV) is a persistent pathogen that causes significant economic losses to the global swine industry. Commercial vaccines provide only partial protection, and no effective therapeutic treatments are currently available. In this study, we demonstrate that hesperetin (Hst), a flavonoid glycoside derived from orange and tangerine peels, inhibits PRRSV replication in a dose-dependent manner in Marc-145 and PAMs cells. Transcriptome analysis further reveals that the anti-PRRSV effects of Hst are associated with the suppression of the P38 MAPK pathway, as Hst significantly downregulates key genes, including NRA41, JUN, FOS, and DUSP1. Subsequent investigations show that Hst inhibits PRRSV replication by downregulating the P38/JUN/FOS signaling cascade. These findings offer valuable insights for the development of novel preventive and therapeutic strategies against PRRSV infection. |
| format | Article |
| id | doaj-art-e0fb239eb01148d88dc2f30c74b667d2 |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-e0fb239eb01148d88dc2f30c74b667d22025-08-20T03:12:01ZengMDPI AGMicroorganisms2076-26072025-02-0113245010.3390/microorganisms13020450Hesperetin Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication by Downregulating the P38/JUN/FOS Pathway In VitroRuiheng Gu0Feike Zhao1Quanying Li2Limin Hou3Guochang Liu4Xueyan Sun5Junyuan Du6Binghu Fang7College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaPorcine reproductive and respiratory syndrome virus (PRRSV) is a persistent pathogen that causes significant economic losses to the global swine industry. Commercial vaccines provide only partial protection, and no effective therapeutic treatments are currently available. In this study, we demonstrate that hesperetin (Hst), a flavonoid glycoside derived from orange and tangerine peels, inhibits PRRSV replication in a dose-dependent manner in Marc-145 and PAMs cells. Transcriptome analysis further reveals that the anti-PRRSV effects of Hst are associated with the suppression of the P38 MAPK pathway, as Hst significantly downregulates key genes, including NRA41, JUN, FOS, and DUSP1. Subsequent investigations show that Hst inhibits PRRSV replication by downregulating the P38/JUN/FOS signaling cascade. These findings offer valuable insights for the development of novel preventive and therapeutic strategies against PRRSV infection.https://www.mdpi.com/2076-2607/13/2/450Hesperetin (Hst)Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)inhibitsP38 MAPK pathwayP38/JUN/FOS |
| spellingShingle | Ruiheng Gu Feike Zhao Quanying Li Limin Hou Guochang Liu Xueyan Sun Junyuan Du Binghu Fang Hesperetin Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication by Downregulating the P38/JUN/FOS Pathway In Vitro Microorganisms Hesperetin (Hst) Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) inhibits P38 MAPK pathway P38/JUN/FOS |
| title | Hesperetin Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication by Downregulating the P38/JUN/FOS Pathway In Vitro |
| title_full | Hesperetin Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication by Downregulating the P38/JUN/FOS Pathway In Vitro |
| title_fullStr | Hesperetin Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication by Downregulating the P38/JUN/FOS Pathway In Vitro |
| title_full_unstemmed | Hesperetin Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication by Downregulating the P38/JUN/FOS Pathway In Vitro |
| title_short | Hesperetin Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication by Downregulating the P38/JUN/FOS Pathway In Vitro |
| title_sort | hesperetin inhibits porcine reproductive and respiratory syndrome virus replication by downregulating the p38 jun fos pathway in vitro |
| topic | Hesperetin (Hst) Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) inhibits P38 MAPK pathway P38/JUN/FOS |
| url | https://www.mdpi.com/2076-2607/13/2/450 |
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