Comparative transcriptomic and phenotypic analysis of monoclonal and polyclonal Populus deltoides genotypes
Populus species are highly valued for bioenergy and bioproducts due to their rapid growth and productivity. Polyclonal plantings, or mixtures of Populus clones, have shown the potential to enhance resource utilization and productivity, likely due to phenotypic differences arising from niche differen...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1498535/full |
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| author | Macy Gosselaar Macy Gosselaar Mark A. Arick Chuan-Yu Hsu Heidi Renninger Courtney M. Siegert Waqar Shafqat Daniel G. Peterson Austin Himes |
| author_facet | Macy Gosselaar Macy Gosselaar Mark A. Arick Chuan-Yu Hsu Heidi Renninger Courtney M. Siegert Waqar Shafqat Daniel G. Peterson Austin Himes |
| author_sort | Macy Gosselaar |
| collection | DOAJ |
| description | Populus species are highly valued for bioenergy and bioproducts due to their rapid growth and productivity. Polyclonal plantings, or mixtures of Populus clones, have shown the potential to enhance resource utilization and productivity, likely due to phenotypic differences arising from niche differentiation. In this study, we investigated gene expression and productivity in monoclonal and polyclonal stands of P. deltoides. Phenotypic results showed that polyclonal plots exhibited higher leaf area index (LAI; p < 0.01, 2.96 ± 0.057 m2) and total biomass (p < 0.01, 2.74 ± 0.06) compared to monoclonal plots, indicating superior productivity. RNA sequencing revealed upregulation of key genes such as exocyst subunit exo70 family protein H7 (EXO70H7), NDH-dependent cyclic electron flow 5 (NDF5), and expansin-like A3 (EXLA3). We also observed enrichment in phenylalanine metabolism and other secondary metabolic pathways in clone S7C8. Phenotypic results, upregulated genes and enriched biological pathways identified in this study may explain the enhanced productivity, increased nitrate content, and expanded canopy in polyclonal plantings. Overall, this study provides a foundation for future research to enhance forest productivity by linking molecular mechanisms to practical applications in field plantings. |
| format | Article |
| id | doaj-art-e0f75c705186475180831a6f341d34aa |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj-art-e0f75c705186475180831a6f341d34aa2025-01-23T06:56:19ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011510.3389/fpls.2024.14985351498535Comparative transcriptomic and phenotypic analysis of monoclonal and polyclonal Populus deltoides genotypesMacy Gosselaar0Macy Gosselaar1Mark A. Arick2Chuan-Yu Hsu3Heidi Renninger4Courtney M. Siegert5Waqar Shafqat6Daniel G. Peterson7Austin Himes8Department of Forestry, Forest and Wildlife Research Center, Mississippi State University, Mississippi State, MS, United StatesInstitute for Genomics, Biocomputing, and Biotechnology, Mississippi State University, Mississippi State, MS, United StatesInstitute for Genomics, Biocomputing, and Biotechnology, Mississippi State University, Mississippi State, MS, United StatesInstitute for Genomics, Biocomputing, and Biotechnology, Mississippi State University, Mississippi State, MS, United StatesDepartment of Forestry, Forest and Wildlife Research Center, Mississippi State University, Mississippi State, MS, United StatesDepartment of Forestry, Forest and Wildlife Research Center, Mississippi State University, Mississippi State, MS, United StatesDepartment of Forestry, Forest and Wildlife Research Center, Mississippi State University, Mississippi State, MS, United StatesInstitute for Genomics, Biocomputing, and Biotechnology, Mississippi State University, Mississippi State, MS, United StatesDepartment of Forestry, Forest and Wildlife Research Center, Mississippi State University, Mississippi State, MS, United StatesPopulus species are highly valued for bioenergy and bioproducts due to their rapid growth and productivity. Polyclonal plantings, or mixtures of Populus clones, have shown the potential to enhance resource utilization and productivity, likely due to phenotypic differences arising from niche differentiation. In this study, we investigated gene expression and productivity in monoclonal and polyclonal stands of P. deltoides. Phenotypic results showed that polyclonal plots exhibited higher leaf area index (LAI; p < 0.01, 2.96 ± 0.057 m2) and total biomass (p < 0.01, 2.74 ± 0.06) compared to monoclonal plots, indicating superior productivity. RNA sequencing revealed upregulation of key genes such as exocyst subunit exo70 family protein H7 (EXO70H7), NDH-dependent cyclic electron flow 5 (NDF5), and expansin-like A3 (EXLA3). We also observed enrichment in phenylalanine metabolism and other secondary metabolic pathways in clone S7C8. Phenotypic results, upregulated genes and enriched biological pathways identified in this study may explain the enhanced productivity, increased nitrate content, and expanded canopy in polyclonal plantings. Overall, this study provides a foundation for future research to enhance forest productivity by linking molecular mechanisms to practical applications in field plantings.https://www.frontiersin.org/articles/10.3389/fpls.2024.1498535/fulleastern cottonwoodniche differentiationdifferential gene expressionnitrogenuse efficiencyproductivity |
| spellingShingle | Macy Gosselaar Macy Gosselaar Mark A. Arick Chuan-Yu Hsu Heidi Renninger Courtney M. Siegert Waqar Shafqat Daniel G. Peterson Austin Himes Comparative transcriptomic and phenotypic analysis of monoclonal and polyclonal Populus deltoides genotypes Frontiers in Plant Science eastern cottonwood niche differentiation differential gene expression nitrogenuse efficiency productivity |
| title | Comparative transcriptomic and phenotypic analysis of monoclonal and polyclonal Populus deltoides genotypes |
| title_full | Comparative transcriptomic and phenotypic analysis of monoclonal and polyclonal Populus deltoides genotypes |
| title_fullStr | Comparative transcriptomic and phenotypic analysis of monoclonal and polyclonal Populus deltoides genotypes |
| title_full_unstemmed | Comparative transcriptomic and phenotypic analysis of monoclonal and polyclonal Populus deltoides genotypes |
| title_short | Comparative transcriptomic and phenotypic analysis of monoclonal and polyclonal Populus deltoides genotypes |
| title_sort | comparative transcriptomic and phenotypic analysis of monoclonal and polyclonal populus deltoides genotypes |
| topic | eastern cottonwood niche differentiation differential gene expression nitrogenuse efficiency productivity |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1498535/full |
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