Challenges in diagnosing bovine tuberculosis through surveillance and characterization of Mycobacterium species in slaughtered cattle in Kolkata
Abstract Background Tuberculosis in cattle is caused by Mycobacterium tuberculosis complex (MTBC) species. Apart from MTBC, different Nontuberculous Mycobacteria (NTM) species have also been isolated from cattle. The presence of NTM infection in bovines makes the diagnosis of bovine tuberculosis (bT...
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BMC
2024-10-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-024-04272-9 |
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| author | Molla Zakirul Haque Chanchal Guha Ayan Mukherjee Sukhen Samanta Partha Sarathi Jana Ujjwal Biswas Sangeeta Mandal Santanu Pal Manigandan Venkatesan Joy Sarojini Michael Pramod Kumar Nanda Samiran Bandyopadhyay Arun K. Das Premanshu Dandapat |
| author_facet | Molla Zakirul Haque Chanchal Guha Ayan Mukherjee Sukhen Samanta Partha Sarathi Jana Ujjwal Biswas Sangeeta Mandal Santanu Pal Manigandan Venkatesan Joy Sarojini Michael Pramod Kumar Nanda Samiran Bandyopadhyay Arun K. Das Premanshu Dandapat |
| author_sort | Molla Zakirul Haque |
| collection | DOAJ |
| description | Abstract Background Tuberculosis in cattle is caused by Mycobacterium tuberculosis complex (MTBC) species. Apart from MTBC, different Nontuberculous Mycobacteria (NTM) species have also been isolated from cattle. The presence of NTM infection in bovines makes the diagnosis of bovine tuberculosis (bTB) a cumbersome task. Therefore, a cross sectional study was conducted to isolate and characterize different Mycobacterium spp. from a slaughterhouse situated in Kolkata, a city in the eastern part of India. Results Out of 258 morbid samples, 98 isolates were found to be positive for bacterial growth, and 35% (n = 34) were positive for Mycobacterium. 94% of Mycobacterial cultural isolates were NTM (n = 32), and the rest (n = 2) were found to be MTBC. Species-level identification of the isolates by hsp65 sequencing revealed that out of 32 isolates, 24 were M. fortuitum, three were M. abscessus, two each were M. chelonae and M. parascrofulaceum, and one was M. novocastrense. A phylogenetic tree with partial hsp65 gene sequences was also constructed to determine the relatedness of the unknown isolates to the reference strains. Conclusion Both NTM species and MTBCs were identified from TB-like lesions in cattle that were slaughtered at the Kolkata abattoir. This discovery may indicate that NTM contributes to the development of lesions in cattle. Also, we recommend implication of more specific diagnostic tests for bTB. |
| format | Article |
| id | doaj-art-e0ea0383e4ab4633bc904ef593b12242 |
| institution | OA Journals |
| issn | 1746-6148 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj-art-e0ea0383e4ab4633bc904ef593b122422025-08-20T01:50:38ZengBMCBMC Veterinary Research1746-61482024-10-0120111010.1186/s12917-024-04272-9Challenges in diagnosing bovine tuberculosis through surveillance and characterization of Mycobacterium species in slaughtered cattle in KolkataMolla Zakirul Haque0Chanchal Guha1Ayan Mukherjee2Sukhen Samanta3Partha Sarathi Jana4Ujjwal Biswas5Sangeeta Mandal6Santanu Pal7Manigandan Venkatesan8Joy Sarojini Michael9Pramod Kumar Nanda10Samiran Bandyopadhyay11Arun K. Das12Premanshu Dandapat13West Bengal University of Animal and Fishery SciencesWest Bengal University of Animal and Fishery SciencesWest Bengal University of Animal and Fishery SciencesICAR-Indian Veterinary Research Institute, Eastern Regional StationWest Bengal University of Animal and Fishery SciencesWest Bengal University of Animal and Fishery SciencesWest Bengal University of Animal and Fishery SciencesICAR-Indian Veterinary Research Institute, Eastern Regional StationChristian Medical CollegeChristian Medical CollegeICAR-Indian Veterinary Research Institute, Eastern Regional StationICAR-Indian Veterinary Research Institute, Eastern Regional StationICAR-Indian Veterinary Research Institute, Eastern Regional StationICAR-Indian Veterinary Research Institute, Eastern Regional StationAbstract Background Tuberculosis in cattle is caused by Mycobacterium tuberculosis complex (MTBC) species. Apart from MTBC, different Nontuberculous Mycobacteria (NTM) species have also been isolated from cattle. The presence of NTM infection in bovines makes the diagnosis of bovine tuberculosis (bTB) a cumbersome task. Therefore, a cross sectional study was conducted to isolate and characterize different Mycobacterium spp. from a slaughterhouse situated in Kolkata, a city in the eastern part of India. Results Out of 258 morbid samples, 98 isolates were found to be positive for bacterial growth, and 35% (n = 34) were positive for Mycobacterium. 94% of Mycobacterial cultural isolates were NTM (n = 32), and the rest (n = 2) were found to be MTBC. Species-level identification of the isolates by hsp65 sequencing revealed that out of 32 isolates, 24 were M. fortuitum, three were M. abscessus, two each were M. chelonae and M. parascrofulaceum, and one was M. novocastrense. A phylogenetic tree with partial hsp65 gene sequences was also constructed to determine the relatedness of the unknown isolates to the reference strains. Conclusion Both NTM species and MTBCs were identified from TB-like lesions in cattle that were slaughtered at the Kolkata abattoir. This discovery may indicate that NTM contributes to the development of lesions in cattle. Also, we recommend implication of more specific diagnostic tests for bTB.https://doi.org/10.1186/s12917-024-04272-9Nontuberculous MycobacteriaAbattoirhsp65Duplex PCRZoonotic bovine tuberculosis |
| spellingShingle | Molla Zakirul Haque Chanchal Guha Ayan Mukherjee Sukhen Samanta Partha Sarathi Jana Ujjwal Biswas Sangeeta Mandal Santanu Pal Manigandan Venkatesan Joy Sarojini Michael Pramod Kumar Nanda Samiran Bandyopadhyay Arun K. Das Premanshu Dandapat Challenges in diagnosing bovine tuberculosis through surveillance and characterization of Mycobacterium species in slaughtered cattle in Kolkata BMC Veterinary Research Nontuberculous Mycobacteria Abattoir hsp65 Duplex PCR Zoonotic bovine tuberculosis |
| title | Challenges in diagnosing bovine tuberculosis through surveillance and characterization of Mycobacterium species in slaughtered cattle in Kolkata |
| title_full | Challenges in diagnosing bovine tuberculosis through surveillance and characterization of Mycobacterium species in slaughtered cattle in Kolkata |
| title_fullStr | Challenges in diagnosing bovine tuberculosis through surveillance and characterization of Mycobacterium species in slaughtered cattle in Kolkata |
| title_full_unstemmed | Challenges in diagnosing bovine tuberculosis through surveillance and characterization of Mycobacterium species in slaughtered cattle in Kolkata |
| title_short | Challenges in diagnosing bovine tuberculosis through surveillance and characterization of Mycobacterium species in slaughtered cattle in Kolkata |
| title_sort | challenges in diagnosing bovine tuberculosis through surveillance and characterization of mycobacterium species in slaughtered cattle in kolkata |
| topic | Nontuberculous Mycobacteria Abattoir hsp65 Duplex PCR Zoonotic bovine tuberculosis |
| url | https://doi.org/10.1186/s12917-024-04272-9 |
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