Elusive elapids: biogeographic venom variation in Indian kraits and its repercussion on snakebite therapy

Snakebite is a major public health concern in many parts of the world, including India, where over 58,000 deaths occur annually due to snake envenoming. The common krait (Bungarus caeruleus) is responsible for the second-highest number of snakebite-related mortalities in the country. However, despit...

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Main Authors: U. Rashmi, Siddharth Bhatia, Muralidhar Nayak, Suyog Khochare, Kartik Sunagar
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-11-01
Series:Frontiers in Pharmacology
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Online Access:https://www.frontiersin.org/articles/10.3389/fphar.2024.1443073/full
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author U. Rashmi
Siddharth Bhatia
Muralidhar Nayak
Suyog Khochare
Kartik Sunagar
author_facet U. Rashmi
Siddharth Bhatia
Muralidhar Nayak
Suyog Khochare
Kartik Sunagar
author_sort U. Rashmi
collection DOAJ
description Snakebite is a major public health concern in many parts of the world, including India, where over 58,000 deaths occur annually due to snake envenoming. The common krait (Bungarus caeruleus) is responsible for the second-highest number of snakebite-related mortalities in the country. However, despite its notoriety, little is known about its venom ecology, functions and compositional variation across bioclimatic zones, partly because these nocturnal snakes are highly elusive, making it difficult to find them in the wild. We aim to address this knowledge gap by characterising the venom composition and toxicity profiles of the pan-Indian populations (n = 8) of B. caeruleus using a combination of proteomics, receptor-toxin interaction assays, biochemical experiments, pharmacological tests and preclinical evaluations. We reveal considerable variation in venom composition, functions, and pharmacological activities among the geographically distinct populations of B. caeruleus. Furthermore, toxin-receptor interaction assays provide insights into their feeding ecology and prey-predator interactions. Finally, in vitro and in vivo experiments revealed the poor neutralising potencies of Indian antivenoms towards most populations of the common krait. Our findings highlight the alarming need to develop efficacious snakebite therapy in India to treat bites from this medically most important elapid snake.
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spelling doaj-art-e0e5d5f3913f41f3a87418ba178fdaff2025-08-20T02:12:47ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122024-11-011510.3389/fphar.2024.14430731443073Elusive elapids: biogeographic venom variation in Indian kraits and its repercussion on snakebite therapyU. RashmiSiddharth BhatiaMuralidhar NayakSuyog KhochareKartik SunagarSnakebite is a major public health concern in many parts of the world, including India, where over 58,000 deaths occur annually due to snake envenoming. The common krait (Bungarus caeruleus) is responsible for the second-highest number of snakebite-related mortalities in the country. However, despite its notoriety, little is known about its venom ecology, functions and compositional variation across bioclimatic zones, partly because these nocturnal snakes are highly elusive, making it difficult to find them in the wild. We aim to address this knowledge gap by characterising the venom composition and toxicity profiles of the pan-Indian populations (n = 8) of B. caeruleus using a combination of proteomics, receptor-toxin interaction assays, biochemical experiments, pharmacological tests and preclinical evaluations. We reveal considerable variation in venom composition, functions, and pharmacological activities among the geographically distinct populations of B. caeruleus. Furthermore, toxin-receptor interaction assays provide insights into their feeding ecology and prey-predator interactions. Finally, in vitro and in vivo experiments revealed the poor neutralising potencies of Indian antivenoms towards most populations of the common krait. Our findings highlight the alarming need to develop efficacious snakebite therapy in India to treat bites from this medically most important elapid snake.https://www.frontiersin.org/articles/10.3389/fphar.2024.1443073/fullBungarus caeruleussnake venomElapidaeIndian antivenomssnakebite
spellingShingle U. Rashmi
Siddharth Bhatia
Muralidhar Nayak
Suyog Khochare
Kartik Sunagar
Elusive elapids: biogeographic venom variation in Indian kraits and its repercussion on snakebite therapy
Frontiers in Pharmacology
Bungarus caeruleus
snake venom
Elapidae
Indian antivenoms
snakebite
title Elusive elapids: biogeographic venom variation in Indian kraits and its repercussion on snakebite therapy
title_full Elusive elapids: biogeographic venom variation in Indian kraits and its repercussion on snakebite therapy
title_fullStr Elusive elapids: biogeographic venom variation in Indian kraits and its repercussion on snakebite therapy
title_full_unstemmed Elusive elapids: biogeographic venom variation in Indian kraits and its repercussion on snakebite therapy
title_short Elusive elapids: biogeographic venom variation in Indian kraits and its repercussion on snakebite therapy
title_sort elusive elapids biogeographic venom variation in indian kraits and its repercussion on snakebite therapy
topic Bungarus caeruleus
snake venom
Elapidae
Indian antivenoms
snakebite
url https://www.frontiersin.org/articles/10.3389/fphar.2024.1443073/full
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