NR1I3 modulates Wnt signaling to promote anterior–posterior axis patterning
Abstract Background Regeneration of missing structures and tissue turnover during homeostasis require positional information to instruct the reestablishment of body axes and to specify new cells to replace aged tissues. Planarians present an ideal model for positional information studies, as they ca...
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| Format: | Article |
| Language: | English |
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BMC
2025-07-01
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| Series: | BMC Biology |
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| Online Access: | https://doi.org/10.1186/s12915-025-02346-0 |
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| author | Yujia Sun Ning Ding Shuqi Li Yuan Zhang Yunfei Qin Zhitai Hao Shoutao Zhang Qingnan Tian |
| author_facet | Yujia Sun Ning Ding Shuqi Li Yuan Zhang Yunfei Qin Zhitai Hao Shoutao Zhang Qingnan Tian |
| author_sort | Yujia Sun |
| collection | DOAJ |
| description | Abstract Background Regeneration of missing structures and tissue turnover during homeostasis require positional information to instruct the reestablishment of body axes and to specify new cells to replace aged tissues. Planarians present an ideal model for positional information studies, as they can regenerate any missing parts and replace aged cells during tissue turnover. Position control genes (PCGs) characterized by constitutive and regional expression in planarians have been identified to instruct regeneration and tissue turnover. However, it is not entirely understood how these genes coordinate in maintaining proper tissue structures. Results Here we report that NR1I3, a nuclear receptor family transcription factor, functions in the establishment and maintenance of the anteroposterior (AP) axis in planarians. NR1I3 RNA interference (RNAi) during regeneration caused ectopic head formation in the posterior blastema, correlated with symmetric notum expression at wounds. Loss of NR1I3 function in intact planarians resulted in ectopic anterior eye formation and the absence or reduction of posterior PCG expression. Notably, the phenotype of two-headed planarians after NR1I3 RNAi can be suppressed by APC, axins, and notum, which are components of the Wnt signaling. Conclusions Our data reveal that NR1I3 in planarians acts as an upstream regulator of Wnt signaling, mediating AP patterning through regulating the activation of notum. |
| format | Article |
| id | doaj-art-e0e5aa50db1840e8ab2ac35aa6d17ec5 |
| institution | Kabale University |
| issn | 1741-7007 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biology |
| spelling | doaj-art-e0e5aa50db1840e8ab2ac35aa6d17ec52025-08-20T03:46:29ZengBMCBMC Biology1741-70072025-07-0123111610.1186/s12915-025-02346-0NR1I3 modulates Wnt signaling to promote anterior–posterior axis patterningYujia Sun0Ning Ding1Shuqi Li2Yuan Zhang3Yunfei Qin4Zhitai Hao5Shoutao Zhang6Qingnan Tian7School of Life Sciences, Zhengzhou UniversitySchool of Life Sciences, Zhengzhou UniversitySchool of Life Sciences, Zhengzhou UniversitySchool of Life Sciences, Zhengzhou UniversityBiotherapy Center, The Third Affiliated Hospital, Sun Yat-Sen UniversityDepartment of Biochemistry and Molecular Pharmacology, School of Medicine, New York UniversitySchool of Life Sciences, Zhengzhou UniversitySchool of Life Sciences, Zhengzhou UniversityAbstract Background Regeneration of missing structures and tissue turnover during homeostasis require positional information to instruct the reestablishment of body axes and to specify new cells to replace aged tissues. Planarians present an ideal model for positional information studies, as they can regenerate any missing parts and replace aged cells during tissue turnover. Position control genes (PCGs) characterized by constitutive and regional expression in planarians have been identified to instruct regeneration and tissue turnover. However, it is not entirely understood how these genes coordinate in maintaining proper tissue structures. Results Here we report that NR1I3, a nuclear receptor family transcription factor, functions in the establishment and maintenance of the anteroposterior (AP) axis in planarians. NR1I3 RNA interference (RNAi) during regeneration caused ectopic head formation in the posterior blastema, correlated with symmetric notum expression at wounds. Loss of NR1I3 function in intact planarians resulted in ectopic anterior eye formation and the absence or reduction of posterior PCG expression. Notably, the phenotype of two-headed planarians after NR1I3 RNAi can be suppressed by APC, axins, and notum, which are components of the Wnt signaling. Conclusions Our data reveal that NR1I3 in planarians acts as an upstream regulator of Wnt signaling, mediating AP patterning through regulating the activation of notum.https://doi.org/10.1186/s12915-025-02346-0Positional informationNR1I3Wnt signalingNotumAP patterning |
| spellingShingle | Yujia Sun Ning Ding Shuqi Li Yuan Zhang Yunfei Qin Zhitai Hao Shoutao Zhang Qingnan Tian NR1I3 modulates Wnt signaling to promote anterior–posterior axis patterning BMC Biology Positional information NR1I3 Wnt signaling Notum AP patterning |
| title | NR1I3 modulates Wnt signaling to promote anterior–posterior axis patterning |
| title_full | NR1I3 modulates Wnt signaling to promote anterior–posterior axis patterning |
| title_fullStr | NR1I3 modulates Wnt signaling to promote anterior–posterior axis patterning |
| title_full_unstemmed | NR1I3 modulates Wnt signaling to promote anterior–posterior axis patterning |
| title_short | NR1I3 modulates Wnt signaling to promote anterior–posterior axis patterning |
| title_sort | nr1i3 modulates wnt signaling to promote anterior posterior axis patterning |
| topic | Positional information NR1I3 Wnt signaling Notum AP patterning |
| url | https://doi.org/10.1186/s12915-025-02346-0 |
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