Proteomics signatures associated with hip arthropathy in ankylosing spondylitis
ObjectiveAnkylosing spondylitis (AS) is a chronic inflammatory disease primarily affecting the axial skeleton and peripheral joints, with hip arthropathy representing a severe complication that critically impairs mobility. While persistent inflammation is a hallmark of AS, the molecular mechanisms d...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-05-01
|
| Series: | Frontiers in Medicine |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2025.1556118/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850278944737067008 |
|---|---|
| author | Xianghui Wen Xianghui Wen Linkai Fang Zena Chen Dong Liu Shenghui Wen Jinwei Li Qiuxia Li Qiujing Wei Shuangyan Cao Peng Zhang Jieruo Gu Jieruo Gu |
| author_facet | Xianghui Wen Xianghui Wen Linkai Fang Zena Chen Dong Liu Shenghui Wen Jinwei Li Qiuxia Li Qiujing Wei Shuangyan Cao Peng Zhang Jieruo Gu Jieruo Gu |
| author_sort | Xianghui Wen |
| collection | DOAJ |
| description | ObjectiveAnkylosing spondylitis (AS) is a chronic inflammatory disease primarily affecting the axial skeleton and peripheral joints, with hip arthropathy representing a severe complication that critically impairs mobility. While persistent inflammation is a hallmark of AS, the molecular mechanisms driving hip involvement remain poorly characterized. This study aimed to identify and validate protein biomarkers associated with hip arthropathy progression in AS through integrated proteomic and functional analyses.MethodsLiquid chromatography-mass spectrometry (LC–MS/MS) was employed to screen for differentially abundant proteins (DAPs) in hip joint tissues from 30 AS patients and 14 non-AS controls. Bioinformatics methods were utilized to screen for and identify key DAPs.ResultsA total of 2,050 proteins were relatively quantified, with 109 DAPs (34 upregulated and 75 downregulated) meeting the criteria of p < 0.05 and a fold change ≥1.5 or ≤0.67. Enriched GO terms represented by DAPs included the Wnt signaling pathway, MAPK cascade, and antigen processing and presentation of exogenous peptide antigen via MHC class I. The main Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways included the PI3K-Akt signaling pathway, ribosome, metabolic pathways, and neutrophil extracellular trap formation. The protein–protein interaction (PPI) network identified ribosomal proteins (RPs), including RPS11, RPS24, RPL35, RPS3A, RPS6, RPS8, RPS14, and RPS7, as highly connected hub proteins. These RPs were significantly enriched in pathways associated with hip arthropathy pathogenesis, particularly osteoblast differentiation and T cell-mediated immune regulation.ConclusionBased on proteomics approaches and bioinformatics analysis, this study discovered DAPs and signaling pathways associated with hip arthropathy in AS. It may provide potential as screening tools or therapeutic targets for AS, warranting further research for validation. |
| format | Article |
| id | doaj-art-e0cf761fdbf54995a07844d6a7ebaec3 |
| institution | OA Journals |
| issn | 2296-858X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj-art-e0cf761fdbf54995a07844d6a7ebaec32025-08-20T01:49:16ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2025-05-011210.3389/fmed.2025.15561181556118Proteomics signatures associated with hip arthropathy in ankylosing spondylitisXianghui Wen0Xianghui Wen1Linkai Fang2Zena Chen3Dong Liu4Shenghui Wen5Jinwei Li6Qiuxia Li7Qiujing Wei8Shuangyan Cao9Peng Zhang10Jieruo Gu11Jieruo Gu12Shenzhen Longhua District Central Hospital, Shenzhen, ChinaShenzhen Longhua Institute of Immunology Transformation, Shenzhen, ChinaDepartment of Rheumatology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaDepartment of Rheumatology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaDepartment of Rheumatology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaDepartment of Rheumatology, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaDepartment of Rheumatology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaDepartment of Rheumatology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaDepartment of Rheumatology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaDepartment of Rheumatology, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, ChinaShenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, ChinaShenzhen Longhua Institute of Immunology Transformation, Shenzhen, ChinaDepartment of Rheumatology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaObjectiveAnkylosing spondylitis (AS) is a chronic inflammatory disease primarily affecting the axial skeleton and peripheral joints, with hip arthropathy representing a severe complication that critically impairs mobility. While persistent inflammation is a hallmark of AS, the molecular mechanisms driving hip involvement remain poorly characterized. This study aimed to identify and validate protein biomarkers associated with hip arthropathy progression in AS through integrated proteomic and functional analyses.MethodsLiquid chromatography-mass spectrometry (LC–MS/MS) was employed to screen for differentially abundant proteins (DAPs) in hip joint tissues from 30 AS patients and 14 non-AS controls. Bioinformatics methods were utilized to screen for and identify key DAPs.ResultsA total of 2,050 proteins were relatively quantified, with 109 DAPs (34 upregulated and 75 downregulated) meeting the criteria of p < 0.05 and a fold change ≥1.5 or ≤0.67. Enriched GO terms represented by DAPs included the Wnt signaling pathway, MAPK cascade, and antigen processing and presentation of exogenous peptide antigen via MHC class I. The main Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways included the PI3K-Akt signaling pathway, ribosome, metabolic pathways, and neutrophil extracellular trap formation. The protein–protein interaction (PPI) network identified ribosomal proteins (RPs), including RPS11, RPS24, RPL35, RPS3A, RPS6, RPS8, RPS14, and RPS7, as highly connected hub proteins. These RPs were significantly enriched in pathways associated with hip arthropathy pathogenesis, particularly osteoblast differentiation and T cell-mediated immune regulation.ConclusionBased on proteomics approaches and bioinformatics analysis, this study discovered DAPs and signaling pathways associated with hip arthropathy in AS. It may provide potential as screening tools or therapeutic targets for AS, warranting further research for validation.https://www.frontiersin.org/articles/10.3389/fmed.2025.1556118/fullankylosing spondylitiship arthropathyproteomicship joint tissuesbiomarker |
| spellingShingle | Xianghui Wen Xianghui Wen Linkai Fang Zena Chen Dong Liu Shenghui Wen Jinwei Li Qiuxia Li Qiujing Wei Shuangyan Cao Peng Zhang Jieruo Gu Jieruo Gu Proteomics signatures associated with hip arthropathy in ankylosing spondylitis Frontiers in Medicine ankylosing spondylitis hip arthropathy proteomics hip joint tissues biomarker |
| title | Proteomics signatures associated with hip arthropathy in ankylosing spondylitis |
| title_full | Proteomics signatures associated with hip arthropathy in ankylosing spondylitis |
| title_fullStr | Proteomics signatures associated with hip arthropathy in ankylosing spondylitis |
| title_full_unstemmed | Proteomics signatures associated with hip arthropathy in ankylosing spondylitis |
| title_short | Proteomics signatures associated with hip arthropathy in ankylosing spondylitis |
| title_sort | proteomics signatures associated with hip arthropathy in ankylosing spondylitis |
| topic | ankylosing spondylitis hip arthropathy proteomics hip joint tissues biomarker |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2025.1556118/full |
| work_keys_str_mv | AT xianghuiwen proteomicssignaturesassociatedwithhiparthropathyinankylosingspondylitis AT xianghuiwen proteomicssignaturesassociatedwithhiparthropathyinankylosingspondylitis AT linkaifang proteomicssignaturesassociatedwithhiparthropathyinankylosingspondylitis AT zenachen proteomicssignaturesassociatedwithhiparthropathyinankylosingspondylitis AT dongliu proteomicssignaturesassociatedwithhiparthropathyinankylosingspondylitis AT shenghuiwen proteomicssignaturesassociatedwithhiparthropathyinankylosingspondylitis AT jinweili proteomicssignaturesassociatedwithhiparthropathyinankylosingspondylitis AT qiuxiali proteomicssignaturesassociatedwithhiparthropathyinankylosingspondylitis AT qiujingwei proteomicssignaturesassociatedwithhiparthropathyinankylosingspondylitis AT shuangyancao proteomicssignaturesassociatedwithhiparthropathyinankylosingspondylitis AT pengzhang proteomicssignaturesassociatedwithhiparthropathyinankylosingspondylitis AT jieruogu proteomicssignaturesassociatedwithhiparthropathyinankylosingspondylitis AT jieruogu proteomicssignaturesassociatedwithhiparthropathyinankylosingspondylitis |