Enhancing Histological Techniques for Small Crustaceans: Evaluation of Fixation, Decalcification, and Enzymatic Digestion in <i>Neocaridina</i> Shrimp

Enhancing Histological Techniques for Small Crustaceans: Evaluation of Fixation, Decalcification, and Enzymatic Digestion in <i>Neocaridina</i> Shrimp

Histological techniques are essential for studying small crustaceans’, such as <i>Neocaridina</i> shrimp, anatomy and physiology. However, their small size and rapid tissue autolysis present challenges for fixation and processing. This study aimed to optimize histological methods for <...

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Bibliographic Details
Main Authors: Rafał Karol Wild, Dobrochna Adamek-Urbańska, Artur Witold Balicki, Wiktoria Cieśla, Jakub Przybyszewski, Maciej Grzegorz Kamaszewski
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Animals
Subjects:
crustacean histology
fixation
decalcification
protocol optimization
Online Access:https://www.mdpi.com/2076-2615/15/12/1715
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