Glycopattern comparison of sperm and exosomes from conventional in vitro fertilization and rescue intracytoplasmic sperm injection: Enhancing sperm motility through exosome‐mediated glycan modulation
Abstract Infertility is a global issue, with male factors contributing to approximately 50% of cases. While assisted reproductive technologies (ART) resolve 30%–50% of cases, 2%–5% of IVF failures require rescue intracytoplasmic sperm injection (RICSI). Sperm glycocalyx is critical for capacitation...
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2025-06-01
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| Online Access: | https://doi.org/10.1002/VIW.20240121 |
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| author | Muhammad Anas Ramzan Zhedong Yang Li Cao Jinyuan Zhang Fei Wu Miaomiao Xin Bo Guo Lumin Wang Lingyu Zhao Yannan Qin Chengcheng Liu |
| author_facet | Muhammad Anas Ramzan Zhedong Yang Li Cao Jinyuan Zhang Fei Wu Miaomiao Xin Bo Guo Lumin Wang Lingyu Zhao Yannan Qin Chengcheng Liu |
| author_sort | Muhammad Anas Ramzan |
| collection | DOAJ |
| description | Abstract Infertility is a global issue, with male factors contributing to approximately 50% of cases. While assisted reproductive technologies (ART) resolve 30%–50% of cases, 2%–5% of IVF failures require rescue intracytoplasmic sperm injection (RICSI). Sperm glycocalyx is critical for capacitation and fertilization, and sperm‐derived exosomes, essential for cell communication, remain underexplored. This study used lectin microarrays and fluorescence lectin histochemistry to analyze glycopatterns in sperm and exosomes from IVF and RICSI groups, and assessed RICSI sperm motility and glycan expression after exposure to IVF‐derived exosomes. Clinical data from 2023 at Northwest Women and Children's Hospital, China, showed that RICSI couples had longer infertility durations, and younger ages, required lower gonadotropin doses, and retrieved more oocytes, despite lower sperm quality. However, pregnancy rates were similar between RICSI and IVF groups. Glycopattern analysis revealed that αGalNAc, Tn antigen, and GalNAcα1‐3(Fucα1‐2)Gal (DBA‐recognized) glycans were downregulated in RICSI sperm, while Galβ1‐3GalNAcα‐Ser/Thr (T), GalNAcα‐Ser/Thr (Tn), and sialyl‐T (Jacalin‐recognized) were upregulated in RICSI exosomes compared to IVF. Exosomes showed higher expression of PHA‐L‐, SBA‐, and UEA‐I‐bound glycans, but lower AAL‐bound glycans than sperm. DBA is primarily bound to the neck, acrosomal region, plasma membrane, and tail of IVF sperm, but its binding was significantly reduced in RICSI sperm. Functionally, IVF exosomes enhanced RICSI sperm motility in a dose‐dependent manner by upregulating DBA‐recognized glycans. These findings provide insights into biomarkers, IVF optimization, and glycosylation's role in sperm and exosome‐related infertility, suggesting exosome‐based therapy for improving RICSI sperm motility. |
| format | Article |
| id | doaj-art-e0ca075b88a64000bc94dbfbc6347d30 |
| institution | DOAJ |
| issn | 2688-3988 2688-268X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Wiley |
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| spelling | doaj-art-e0ca075b88a64000bc94dbfbc6347d302025-08-20T03:22:04ZengWileyView2688-39882688-268X2025-06-0163n/an/a10.1002/VIW.20240121Glycopattern comparison of sperm and exosomes from conventional in vitro fertilization and rescue intracytoplasmic sperm injection: Enhancing sperm motility through exosome‐mediated glycan modulationMuhammad Anas Ramzan0Zhedong Yang1Li Cao2Jinyuan Zhang3Fei Wu4Miaomiao Xin5Bo Guo6Lumin Wang7Lingyu Zhao8Yannan Qin9Chengcheng Liu10Institute of Genetics and Development BiologyTranslational Medicine InstituteXi'an Jiaotong UniversityXi'anChinaInstitute of Genetics and Development BiologyTranslational Medicine InstituteXi'an Jiaotong UniversityXi'anChinaInstitute of Genetics and Development BiologyTranslational Medicine InstituteXi'an Jiaotong UniversityXi'anChinaInstitute of Genetics and Development BiologyTranslational Medicine InstituteXi'an Jiaotong UniversityXi'anChinaDepartment of OncologyThe Second Affiliated HospitalXi'an Jiaotong UniversityXi'anChinaReproductive Medicine Center of Northwest Women's and Children's HospitalXi'anChinaInstitute of Genetics and Development BiologyTranslational Medicine InstituteXi'an Jiaotong UniversityXi'anChinaDepartment of OncologyThe Second Affiliated HospitalXi'an Jiaotong UniversityXi'anChinaInstitute of Genetics and Development BiologyTranslational Medicine InstituteXi'an Jiaotong UniversityXi'anChinaInstitute of Genetics and Development BiologyTranslational Medicine InstituteXi'an Jiaotong UniversityXi'anChinaInstitute of Infection and ImmunityTranslational Medicine InstituteXi'an Jiaotong UniversityXi'anChinaAbstract Infertility is a global issue, with male factors contributing to approximately 50% of cases. While assisted reproductive technologies (ART) resolve 30%–50% of cases, 2%–5% of IVF failures require rescue intracytoplasmic sperm injection (RICSI). Sperm glycocalyx is critical for capacitation and fertilization, and sperm‐derived exosomes, essential for cell communication, remain underexplored. This study used lectin microarrays and fluorescence lectin histochemistry to analyze glycopatterns in sperm and exosomes from IVF and RICSI groups, and assessed RICSI sperm motility and glycan expression after exposure to IVF‐derived exosomes. Clinical data from 2023 at Northwest Women and Children's Hospital, China, showed that RICSI couples had longer infertility durations, and younger ages, required lower gonadotropin doses, and retrieved more oocytes, despite lower sperm quality. However, pregnancy rates were similar between RICSI and IVF groups. Glycopattern analysis revealed that αGalNAc, Tn antigen, and GalNAcα1‐3(Fucα1‐2)Gal (DBA‐recognized) glycans were downregulated in RICSI sperm, while Galβ1‐3GalNAcα‐Ser/Thr (T), GalNAcα‐Ser/Thr (Tn), and sialyl‐T (Jacalin‐recognized) were upregulated in RICSI exosomes compared to IVF. Exosomes showed higher expression of PHA‐L‐, SBA‐, and UEA‐I‐bound glycans, but lower AAL‐bound glycans than sperm. DBA is primarily bound to the neck, acrosomal region, plasma membrane, and tail of IVF sperm, but its binding was significantly reduced in RICSI sperm. Functionally, IVF exosomes enhanced RICSI sperm motility in a dose‐dependent manner by upregulating DBA‐recognized glycans. These findings provide insights into biomarkers, IVF optimization, and glycosylation's role in sperm and exosome‐related infertility, suggesting exosome‐based therapy for improving RICSI sperm motility.https://doi.org/10.1002/VIW.20240121glycopatternin vitro fertilizationlectin microarrayrescue intracytoplasmic sperm injectionspermsperm‐derived exosome |
| spellingShingle | Muhammad Anas Ramzan Zhedong Yang Li Cao Jinyuan Zhang Fei Wu Miaomiao Xin Bo Guo Lumin Wang Lingyu Zhao Yannan Qin Chengcheng Liu Glycopattern comparison of sperm and exosomes from conventional in vitro fertilization and rescue intracytoplasmic sperm injection: Enhancing sperm motility through exosome‐mediated glycan modulation View glycopattern in vitro fertilization lectin microarray rescue intracytoplasmic sperm injection sperm sperm‐derived exosome |
| title | Glycopattern comparison of sperm and exosomes from conventional in vitro fertilization and rescue intracytoplasmic sperm injection: Enhancing sperm motility through exosome‐mediated glycan modulation |
| title_full | Glycopattern comparison of sperm and exosomes from conventional in vitro fertilization and rescue intracytoplasmic sperm injection: Enhancing sperm motility through exosome‐mediated glycan modulation |
| title_fullStr | Glycopattern comparison of sperm and exosomes from conventional in vitro fertilization and rescue intracytoplasmic sperm injection: Enhancing sperm motility through exosome‐mediated glycan modulation |
| title_full_unstemmed | Glycopattern comparison of sperm and exosomes from conventional in vitro fertilization and rescue intracytoplasmic sperm injection: Enhancing sperm motility through exosome‐mediated glycan modulation |
| title_short | Glycopattern comparison of sperm and exosomes from conventional in vitro fertilization and rescue intracytoplasmic sperm injection: Enhancing sperm motility through exosome‐mediated glycan modulation |
| title_sort | glycopattern comparison of sperm and exosomes from conventional in vitro fertilization and rescue intracytoplasmic sperm injection enhancing sperm motility through exosome mediated glycan modulation |
| topic | glycopattern in vitro fertilization lectin microarray rescue intracytoplasmic sperm injection sperm sperm‐derived exosome |
| url | https://doi.org/10.1002/VIW.20240121 |
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