Assessing mesh size and diffusion of alginate bioinks: A crucial factor for successful bioprinting functional pancreatic islets
Three-dimensional (3D) bioprinting has been utilised for the encapsulation of pancreatic islets for potentially treating type 1 diabetes. A crucial factor in selecting a cell compatible bioink, that maintains islet functionality, is the mesh size and diffusion capacity of the bioink. In this study,...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-10-01
|
| Series: | Materials Today Bio |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425007458 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849390644491976704 |
|---|---|
| author | Carolin Hermanns Rick H.W. de Vries Timo Rademakers Adam Stell Denise F.A. de Bont Omar Paulino da Silva Filho Marlon J. Jetten Carlos D. Mota Sami G. Mohammed Vijayaganapathy Vaithilingam Aart A. van Apeldoorn |
| author_facet | Carolin Hermanns Rick H.W. de Vries Timo Rademakers Adam Stell Denise F.A. de Bont Omar Paulino da Silva Filho Marlon J. Jetten Carlos D. Mota Sami G. Mohammed Vijayaganapathy Vaithilingam Aart A. van Apeldoorn |
| author_sort | Carolin Hermanns |
| collection | DOAJ |
| description | Three-dimensional (3D) bioprinting has been utilised for the encapsulation of pancreatic islets for potentially treating type 1 diabetes. A crucial factor in selecting a cell compatible bioink, that maintains islet functionality, is the mesh size and diffusion capacity of the bioink. In this study, we present a screening strategy for alginate hydrogel formulations in three-dimensional bioprinting, utilizing the fluorescent recovery after photobleaching (FRAP) method and measuring the mesh size of the hydrogels. Subsequently, the 1.5 % alginate formulation that had been selected was used to bioprint the INS1E cell line, primary rat islets, or human islets. It was demonstrated that cell viability and functionality were maintained in all cell sources. This was evidenced by the observation that bioprinted pancreatic islets exhibited a response to physiological glucose levels. The present study indicates that both FRAP and hydrogel mesh size measurements are effective tools for predicting the diffusion of hormones through a hydrogel. These measures should therefore be incorporated into future screenings of hydrogel compositions for the 3D bioprinting of islets. |
| format | Article |
| id | doaj-art-e0c5e535078c4487a61a473fff2d6a24 |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-e0c5e535078c4487a61a473fff2d6a242025-08-20T03:41:26ZengElsevierMaterials Today Bio2590-00642025-10-013410217510.1016/j.mtbio.2025.102175Assessing mesh size and diffusion of alginate bioinks: A crucial factor for successful bioprinting functional pancreatic isletsCarolin Hermanns0Rick H.W. de Vries1Timo Rademakers2Adam Stell3Denise F.A. de Bont4Omar Paulino da Silva Filho5Marlon J. Jetten6Carlos D. Mota7Sami G. Mohammed8Vijayaganapathy Vaithilingam9Aart A. van Apeldoorn10Department of Cell Biology – Inspired Tissue Engineering (cBITE), MERLN Institute for Technology Inspired Regenerative Medicine, Maastricht University, Maastricht, the NetherlandsDepartment of Cell Biology – Inspired Tissue Engineering (cBITE), MERLN Institute for Technology Inspired Regenerative Medicine, Maastricht University, Maastricht, the NetherlandsMERLN Institute for Technology Inspired Regenerative Medicine, Maastricht University, Maastricht, the NetherlandsDepartment of Cell Biology – Inspired Tissue Engineering (cBITE), MERLN Institute for Technology Inspired Regenerative Medicine, Maastricht University, Maastricht, the NetherlandsDepartment of Cell Biology – Inspired Tissue Engineering (cBITE), MERLN Institute for Technology Inspired Regenerative Medicine, Maastricht University, Maastricht, the NetherlandsDepartment of Cell Biology – Inspired Tissue Engineering (cBITE), MERLN Institute for Technology Inspired Regenerative Medicine, Maastricht University, Maastricht, the NetherlandsDepartment of Cell Biology – Inspired Tissue Engineering (cBITE), MERLN Institute for Technology Inspired Regenerative Medicine, Maastricht University, Maastricht, the NetherlandsDepartment of Complex Tissue Regeneration (CTR), MERLN Institute for Technology Inspired Regenerative Medicine, Maastricht University, Maastricht, the NetherlandsDepartment of Cell Biology – Inspired Tissue Engineering (cBITE), MERLN Institute for Technology Inspired Regenerative Medicine, Maastricht University, Maastricht, the NetherlandsDepartment of Cell Biology – Inspired Tissue Engineering (cBITE), MERLN Institute for Technology Inspired Regenerative Medicine, Maastricht University, Maastricht, the NetherlandsDepartment of Cell Biology – Inspired Tissue Engineering (cBITE), MERLN Institute for Technology Inspired Regenerative Medicine, Maastricht University, Maastricht, the Netherlands; Corresponding author. Department of Cell Biology–Inspired Tissue Engineering, MERLN Institute, Maastricht University, PO Box 616, 6200 MD, Maastricht, the Netherlands.Three-dimensional (3D) bioprinting has been utilised for the encapsulation of pancreatic islets for potentially treating type 1 diabetes. A crucial factor in selecting a cell compatible bioink, that maintains islet functionality, is the mesh size and diffusion capacity of the bioink. In this study, we present a screening strategy for alginate hydrogel formulations in three-dimensional bioprinting, utilizing the fluorescent recovery after photobleaching (FRAP) method and measuring the mesh size of the hydrogels. Subsequently, the 1.5 % alginate formulation that had been selected was used to bioprint the INS1E cell line, primary rat islets, or human islets. It was demonstrated that cell viability and functionality were maintained in all cell sources. This was evidenced by the observation that bioprinted pancreatic islets exhibited a response to physiological glucose levels. The present study indicates that both FRAP and hydrogel mesh size measurements are effective tools for predicting the diffusion of hormones through a hydrogel. These measures should therefore be incorporated into future screenings of hydrogel compositions for the 3D bioprinting of islets.http://www.sciencedirect.com/science/article/pii/S2590006425007458BioprintingFRAPRheologyType 1 diabetes mellitus |
| spellingShingle | Carolin Hermanns Rick H.W. de Vries Timo Rademakers Adam Stell Denise F.A. de Bont Omar Paulino da Silva Filho Marlon J. Jetten Carlos D. Mota Sami G. Mohammed Vijayaganapathy Vaithilingam Aart A. van Apeldoorn Assessing mesh size and diffusion of alginate bioinks: A crucial factor for successful bioprinting functional pancreatic islets Materials Today Bio Bioprinting FRAP Rheology Type 1 diabetes mellitus |
| title | Assessing mesh size and diffusion of alginate bioinks: A crucial factor for successful bioprinting functional pancreatic islets |
| title_full | Assessing mesh size and diffusion of alginate bioinks: A crucial factor for successful bioprinting functional pancreatic islets |
| title_fullStr | Assessing mesh size and diffusion of alginate bioinks: A crucial factor for successful bioprinting functional pancreatic islets |
| title_full_unstemmed | Assessing mesh size and diffusion of alginate bioinks: A crucial factor for successful bioprinting functional pancreatic islets |
| title_short | Assessing mesh size and diffusion of alginate bioinks: A crucial factor for successful bioprinting functional pancreatic islets |
| title_sort | assessing mesh size and diffusion of alginate bioinks a crucial factor for successful bioprinting functional pancreatic islets |
| topic | Bioprinting FRAP Rheology Type 1 diabetes mellitus |
| url | http://www.sciencedirect.com/science/article/pii/S2590006425007458 |
| work_keys_str_mv | AT carolinhermanns assessingmeshsizeanddiffusionofalginatebioinksacrucialfactorforsuccessfulbioprintingfunctionalpancreaticislets AT rickhwdevries assessingmeshsizeanddiffusionofalginatebioinksacrucialfactorforsuccessfulbioprintingfunctionalpancreaticislets AT timorademakers assessingmeshsizeanddiffusionofalginatebioinksacrucialfactorforsuccessfulbioprintingfunctionalpancreaticislets AT adamstell assessingmeshsizeanddiffusionofalginatebioinksacrucialfactorforsuccessfulbioprintingfunctionalpancreaticislets AT denisefadebont assessingmeshsizeanddiffusionofalginatebioinksacrucialfactorforsuccessfulbioprintingfunctionalpancreaticislets AT omarpaulinodasilvafilho assessingmeshsizeanddiffusionofalginatebioinksacrucialfactorforsuccessfulbioprintingfunctionalpancreaticislets AT marlonjjetten assessingmeshsizeanddiffusionofalginatebioinksacrucialfactorforsuccessfulbioprintingfunctionalpancreaticislets AT carlosdmota assessingmeshsizeanddiffusionofalginatebioinksacrucialfactorforsuccessfulbioprintingfunctionalpancreaticislets AT samigmohammed assessingmeshsizeanddiffusionofalginatebioinksacrucialfactorforsuccessfulbioprintingfunctionalpancreaticislets AT vijayaganapathyvaithilingam assessingmeshsizeanddiffusionofalginatebioinksacrucialfactorforsuccessfulbioprintingfunctionalpancreaticislets AT aartavanapeldoorn assessingmeshsizeanddiffusionofalginatebioinksacrucialfactorforsuccessfulbioprintingfunctionalpancreaticislets |