Overexpression of CXCL14 Alleviates Ventilator-Induced Lung Injury through the Downregulation of PKM2-Mediated Cytokine Production

Ventilator-induced lung injury (VILI) is one of the most common complications of mechanical ventilation (MV), which strongly impacts the outcome of ventilated patients. Current evidences indicated that inflammation is a major contributor to the pathogenesis of VILI. Our results showed that MV induce...

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Main Authors: Rui Zhu, Yan-Qing Lei, Dong-Chi Zhao
Format: Article
Language:English
Published: Wiley 2020-01-01
Series:Mediators of Inflammation
Online Access:http://dx.doi.org/10.1155/2020/7650978
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author Rui Zhu
Yan-Qing Lei
Dong-Chi Zhao
author_facet Rui Zhu
Yan-Qing Lei
Dong-Chi Zhao
author_sort Rui Zhu
collection DOAJ
description Ventilator-induced lung injury (VILI) is one of the most common complications of mechanical ventilation (MV), which strongly impacts the outcome of ventilated patients. Current evidences indicated that inflammation is a major contributor to the pathogenesis of VILI. Our results showed that MV induced excessive proinflammatory cytokine productions together with decreased CXCL14 and increased PKM2 expressions in injured lungs. In addition, CXCL14 overexpression downregulated PKM2 expression and attenuated VILI with reduced inflammation. Moreover, the overexpression of PKM2 markedly diminished the protective effects of CXCL14 against VILI as reflected by worsened morphology and increased cytokine production, whereas PKM2 knockdown decreased cytokine production and attenuated VILI. Collectively, these results suggested that CXCL14 overexpression attenuates VILI through the downregulation of PKM2-mediated proinflammatory cytokine production.
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institution Kabale University
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publishDate 2020-01-01
publisher Wiley
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series Mediators of Inflammation
spelling doaj-art-e0c2a17628864c9d926c30e624bbf61a2025-08-20T03:25:59ZengWileyMediators of Inflammation0962-93511466-18612020-01-01202010.1155/2020/76509787650978Overexpression of CXCL14 Alleviates Ventilator-Induced Lung Injury through the Downregulation of PKM2-Mediated Cytokine ProductionRui Zhu0Yan-Qing Lei1Dong-Chi Zhao2Department of Pediatrics, Zhongnan Hospital of Wuhan University, 169 Donghu Road, Wuhan, Hubei 430071, ChinaDepartment of Emergency, Xiangyang No.1 People’s Hospital, Hubei University of Medicine, 15 Jiefang Road, Xiangyang, Hubei 441000, ChinaDepartment of Pediatrics, Zhongnan Hospital of Wuhan University, 169 Donghu Road, Wuhan, Hubei 430071, ChinaVentilator-induced lung injury (VILI) is one of the most common complications of mechanical ventilation (MV), which strongly impacts the outcome of ventilated patients. Current evidences indicated that inflammation is a major contributor to the pathogenesis of VILI. Our results showed that MV induced excessive proinflammatory cytokine productions together with decreased CXCL14 and increased PKM2 expressions in injured lungs. In addition, CXCL14 overexpression downregulated PKM2 expression and attenuated VILI with reduced inflammation. Moreover, the overexpression of PKM2 markedly diminished the protective effects of CXCL14 against VILI as reflected by worsened morphology and increased cytokine production, whereas PKM2 knockdown decreased cytokine production and attenuated VILI. Collectively, these results suggested that CXCL14 overexpression attenuates VILI through the downregulation of PKM2-mediated proinflammatory cytokine production.http://dx.doi.org/10.1155/2020/7650978
spellingShingle Rui Zhu
Yan-Qing Lei
Dong-Chi Zhao
Overexpression of CXCL14 Alleviates Ventilator-Induced Lung Injury through the Downregulation of PKM2-Mediated Cytokine Production
Mediators of Inflammation
title Overexpression of CXCL14 Alleviates Ventilator-Induced Lung Injury through the Downregulation of PKM2-Mediated Cytokine Production
title_full Overexpression of CXCL14 Alleviates Ventilator-Induced Lung Injury through the Downregulation of PKM2-Mediated Cytokine Production
title_fullStr Overexpression of CXCL14 Alleviates Ventilator-Induced Lung Injury through the Downregulation of PKM2-Mediated Cytokine Production
title_full_unstemmed Overexpression of CXCL14 Alleviates Ventilator-Induced Lung Injury through the Downregulation of PKM2-Mediated Cytokine Production
title_short Overexpression of CXCL14 Alleviates Ventilator-Induced Lung Injury through the Downregulation of PKM2-Mediated Cytokine Production
title_sort overexpression of cxcl14 alleviates ventilator induced lung injury through the downregulation of pkm2 mediated cytokine production
url http://dx.doi.org/10.1155/2020/7650978
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AT yanqinglei overexpressionofcxcl14alleviatesventilatorinducedlunginjurythroughthedownregulationofpkm2mediatedcytokineproduction
AT dongchizhao overexpressionofcxcl14alleviatesventilatorinducedlunginjurythroughthedownregulationofpkm2mediatedcytokineproduction