Digital PCR characterizes epithelial cell populations in murine duodenal organoids.
Three-dimensional cultures are powerful tools to recapitulate animal and human tissues. Under the influence of specific growth factors, adult stem cells differentiate and organize into 3D cultures named organoids. The molecular phenotyping of these structures is an essential step for validating an o...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2025-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0319701 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849732111770058752 |
|---|---|
| author | Karla Acosta-Virgen Hugo David González-Conchillos Gabriela Vallejo-Flores Lizbeth Iliana Salazar-Villatoro Ernesto Guerrero-Sánchez Adolfo Martínez-Palomo Martha Espinosa-Cantellano |
| author_facet | Karla Acosta-Virgen Hugo David González-Conchillos Gabriela Vallejo-Flores Lizbeth Iliana Salazar-Villatoro Ernesto Guerrero-Sánchez Adolfo Martínez-Palomo Martha Espinosa-Cantellano |
| author_sort | Karla Acosta-Virgen |
| collection | DOAJ |
| description | Three-dimensional cultures are powerful tools to recapitulate animal and human tissues. Under the influence of specific growth factors, adult stem cells differentiate and organize into 3D cultures named organoids. The molecular phenotyping of these structures is an essential step for validating an organoid model. However, the limited number of organoids generated in culture yields very low amounts of genetic material, making phenotyping difficult. Recently, digital PCR (dPCR) techniques have become available for the highly sensitive detection of genetic material at low concentrations. The aim of this work was to apply dPCR to the identification of the various cell populations expected to be present in murine duodenal organoids. Results show the potential use of dPCR as a genetic characterization tool for organoids. |
| format | Article |
| id | doaj-art-e0bdf97d1850410e970209603b430e06 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-e0bdf97d1850410e970209603b430e062025-08-20T03:08:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01203e031970110.1371/journal.pone.0319701Digital PCR characterizes epithelial cell populations in murine duodenal organoids.Karla Acosta-VirgenHugo David González-ConchillosGabriela Vallejo-FloresLizbeth Iliana Salazar-VillatoroErnesto Guerrero-SánchezAdolfo Martínez-PalomoMartha Espinosa-CantellanoThree-dimensional cultures are powerful tools to recapitulate animal and human tissues. Under the influence of specific growth factors, adult stem cells differentiate and organize into 3D cultures named organoids. The molecular phenotyping of these structures is an essential step for validating an organoid model. However, the limited number of organoids generated in culture yields very low amounts of genetic material, making phenotyping difficult. Recently, digital PCR (dPCR) techniques have become available for the highly sensitive detection of genetic material at low concentrations. The aim of this work was to apply dPCR to the identification of the various cell populations expected to be present in murine duodenal organoids. Results show the potential use of dPCR as a genetic characterization tool for organoids.https://doi.org/10.1371/journal.pone.0319701 |
| spellingShingle | Karla Acosta-Virgen Hugo David González-Conchillos Gabriela Vallejo-Flores Lizbeth Iliana Salazar-Villatoro Ernesto Guerrero-Sánchez Adolfo Martínez-Palomo Martha Espinosa-Cantellano Digital PCR characterizes epithelial cell populations in murine duodenal organoids. PLoS ONE |
| title | Digital PCR characterizes epithelial cell populations in murine duodenal organoids. |
| title_full | Digital PCR characterizes epithelial cell populations in murine duodenal organoids. |
| title_fullStr | Digital PCR characterizes epithelial cell populations in murine duodenal organoids. |
| title_full_unstemmed | Digital PCR characterizes epithelial cell populations in murine duodenal organoids. |
| title_short | Digital PCR characterizes epithelial cell populations in murine duodenal organoids. |
| title_sort | digital pcr characterizes epithelial cell populations in murine duodenal organoids |
| url | https://doi.org/10.1371/journal.pone.0319701 |
| work_keys_str_mv | AT karlaacostavirgen digitalpcrcharacterizesepithelialcellpopulationsinmurineduodenalorganoids AT hugodavidgonzalezconchillos digitalpcrcharacterizesepithelialcellpopulationsinmurineduodenalorganoids AT gabrielavallejoflores digitalpcrcharacterizesepithelialcellpopulationsinmurineduodenalorganoids AT lizbethilianasalazarvillatoro digitalpcrcharacterizesepithelialcellpopulationsinmurineduodenalorganoids AT ernestoguerrerosanchez digitalpcrcharacterizesepithelialcellpopulationsinmurineduodenalorganoids AT adolfomartinezpalomo digitalpcrcharacterizesepithelialcellpopulationsinmurineduodenalorganoids AT marthaespinosacantellano digitalpcrcharacterizesepithelialcellpopulationsinmurineduodenalorganoids |