Programmed Transformation of Osteogenesis Microenvironment by a Multifunctional Hydrogel to Enhance Repair of Infectious Bone Defects
Abstract Repair of infectious bone defects remains a serious problem in clinical practice owing to the high risk of infection and excessive reactive oxygen species (ROS) during the early stage, and the residual bacteria and delayed Osseo integrated interface in the later stage, which jointly creates...
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Wiley
2025-03-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202409683 |
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| author | En Xie Zhangqin Yuan Qianglong Chen Jie Hu Jiaying Li Kexin Li Huan Wang Jinjin Ma Bin Meng Ruoxi Zhang Haijiao Mao Ting Liang Lijie Wang Chaoyong Liu Bin Li Fengxuan Han |
| author_facet | En Xie Zhangqin Yuan Qianglong Chen Jie Hu Jiaying Li Kexin Li Huan Wang Jinjin Ma Bin Meng Ruoxi Zhang Haijiao Mao Ting Liang Lijie Wang Chaoyong Liu Bin Li Fengxuan Han |
| author_sort | En Xie |
| collection | DOAJ |
| description | Abstract Repair of infectious bone defects remains a serious problem in clinical practice owing to the high risk of infection and excessive reactive oxygen species (ROS) during the early stage, and the residual bacteria and delayed Osseo integrated interface in the later stage, which jointly creates a complex and dynamic microenvironment and leads to bone non‐union. The melatonin carbon dots (MCDs) possess antibacterial and osteogenesis abilities, greatly simplifying the composition of a multifunctional material. Therefore, a multifunctional hydrogel containing MCDs (GH‐MCD) is developed to meet the multi‐stage and complex repair needs of infectious bone injury in this study. The GH‐MCD can intelligently release MCDs responding to the acidic microenvironment to scavenge intracellular ROS and exhibit good antibacterial activity by inducing the production of ROS in bacteria and inhibiting the expression of secA2. Moreover, it has high osteogenesis and long‐lasting antimicrobial activity during bone repair. RNA‐seq results reveal that the hydrogels promote the repair of infected bone healing by enhancing cellular resistance to bacteria, balancing osteogenesis and osteoclastogenesis, and regulating the immune microenvironment. In conclusion, the GH‐MCD can promote the repair of infectious bone defects through the programmed transformation of the microenvironment, providing a novel strategy for infectious bone defects. |
| format | Article |
| id | doaj-art-e0a70a9431a74d1d9aa1a9730625efde |
| institution | DOAJ |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-e0a70a9431a74d1d9aa1a9730625efde2025-08-20T03:10:53ZengWileyAdvanced Science2198-38442025-03-011210n/an/a10.1002/advs.202409683Programmed Transformation of Osteogenesis Microenvironment by a Multifunctional Hydrogel to Enhance Repair of Infectious Bone DefectsEn Xie0Zhangqin Yuan1Qianglong Chen2Jie Hu3Jiaying Li4Kexin Li5Huan Wang6Jinjin Ma7Bin Meng8Ruoxi Zhang9Haijiao Mao10Ting Liang11Lijie Wang12Chaoyong Liu13Bin Li14Fengxuan Han15Orthopedic Institute Department of Orthopedic Surgery Medical 3D Printing Center The First Affiliated Hospital Changzhou Geriatric hospital MOE Key Laboratory of Geriatric Diseases and Immunology, School of Basic Medical Sciences Suzhou Medical College Soochow University Suzhou Jiangsu 215000 P. R. ChinaOrthopedic Institute Department of Orthopedic Surgery Medical 3D Printing Center The First Affiliated Hospital Changzhou Geriatric hospital MOE Key Laboratory of Geriatric Diseases and Immunology, School of Basic Medical Sciences Suzhou Medical College Soochow University Suzhou Jiangsu 215000 P. R. ChinaOrthopedic Institute Department of Orthopedic Surgery Medical 3D Printing Center The First Affiliated Hospital Changzhou Geriatric hospital MOE Key Laboratory of Geriatric Diseases and Immunology, School of Basic Medical Sciences Suzhou Medical College Soochow University Suzhou Jiangsu 215000 P. R. ChinaOrthopedic Institute Department of Orthopedic Surgery Medical 3D Printing Center The First Affiliated Hospital Changzhou Geriatric hospital MOE Key Laboratory of Geriatric Diseases and Immunology, School of Basic Medical Sciences Suzhou Medical College Soochow University Suzhou Jiangsu 215000 P. R. ChinaOrthopedic Institute Department of Orthopedic Surgery Medical 3D Printing Center The First Affiliated Hospital Changzhou Geriatric hospital MOE Key Laboratory of Geriatric Diseases and Immunology, School of Basic Medical Sciences Suzhou Medical College Soochow University Suzhou Jiangsu 215000 P. R. ChinaOrthopedic Institute Department of Orthopedic Surgery Medical 3D Printing Center The First Affiliated Hospital Changzhou Geriatric hospital MOE Key Laboratory of Geriatric Diseases and Immunology, School of Basic Medical Sciences Suzhou Medical College Soochow University Suzhou Jiangsu 215000 P. R. ChinaOrthopedic Institute Department of Orthopedic Surgery Medical 3D Printing Center The First Affiliated Hospital Changzhou Geriatric hospital MOE Key Laboratory of Geriatric Diseases and Immunology, School of Basic Medical Sciences Suzhou Medical College Soochow University Suzhou Jiangsu 215000 P. R. ChinaOrthopedic Institute Department of Orthopedic Surgery Medical 3D Printing Center The First Affiliated Hospital Changzhou Geriatric hospital MOE Key Laboratory of Geriatric Diseases and Immunology, School of Basic Medical Sciences Suzhou Medical College Soochow University Suzhou Jiangsu 215000 P. R. ChinaOrthopedic Institute Department of Orthopedic Surgery Medical 3D Printing Center The First Affiliated Hospital Changzhou Geriatric hospital MOE Key Laboratory of Geriatric Diseases and Immunology, School of Basic Medical Sciences Suzhou Medical College Soochow University Suzhou Jiangsu 215000 P. R. ChinaBeijing Advanced Innovation Center for Soft Matter Science and Engineering College of Life Science and Technology Beijing University of Chemical Technology Beijing 100029 P. R. ChinaDepartment of Orthopaedic Surgery The First Affiliated Hospital of Ningbo University Ningbo Zhejiang 315020 P. R. ChinaOrthopedic Institute Department of Orthopedic Surgery Medical 3D Printing Center The First Affiliated Hospital Changzhou Geriatric hospital MOE Key Laboratory of Geriatric Diseases and Immunology, School of Basic Medical Sciences Suzhou Medical College Soochow University Suzhou Jiangsu 215000 P. R. ChinaSanitation & Environment Technology Institute of Soochow University Ltd. Suzhou Jiangsu 215000 P. R. ChinaBeijing Advanced Innovation Center for Soft Matter Science and Engineering College of Life Science and Technology Beijing University of Chemical Technology Beijing 100029 P. R. ChinaOrthopedic Institute Department of Orthopedic Surgery Medical 3D Printing Center The First Affiliated Hospital Changzhou Geriatric hospital MOE Key Laboratory of Geriatric Diseases and Immunology, School of Basic Medical Sciences Suzhou Medical College Soochow University Suzhou Jiangsu 215000 P. R. ChinaOrthopedic Institute Department of Orthopedic Surgery Medical 3D Printing Center The First Affiliated Hospital Changzhou Geriatric hospital MOE Key Laboratory of Geriatric Diseases and Immunology, School of Basic Medical Sciences Suzhou Medical College Soochow University Suzhou Jiangsu 215000 P. R. ChinaAbstract Repair of infectious bone defects remains a serious problem in clinical practice owing to the high risk of infection and excessive reactive oxygen species (ROS) during the early stage, and the residual bacteria and delayed Osseo integrated interface in the later stage, which jointly creates a complex and dynamic microenvironment and leads to bone non‐union. The melatonin carbon dots (MCDs) possess antibacterial and osteogenesis abilities, greatly simplifying the composition of a multifunctional material. Therefore, a multifunctional hydrogel containing MCDs (GH‐MCD) is developed to meet the multi‐stage and complex repair needs of infectious bone injury in this study. The GH‐MCD can intelligently release MCDs responding to the acidic microenvironment to scavenge intracellular ROS and exhibit good antibacterial activity by inducing the production of ROS in bacteria and inhibiting the expression of secA2. Moreover, it has high osteogenesis and long‐lasting antimicrobial activity during bone repair. RNA‐seq results reveal that the hydrogels promote the repair of infected bone healing by enhancing cellular resistance to bacteria, balancing osteogenesis and osteoclastogenesis, and regulating the immune microenvironment. In conclusion, the GH‐MCD can promote the repair of infectious bone defects through the programmed transformation of the microenvironment, providing a novel strategy for infectious bone defects.https://doi.org/10.1002/advs.202409683antimicrobial propertyhydrogelinfected bone defectsmelatonin carbon dotmicroenvironmentosteogenesis |
| spellingShingle | En Xie Zhangqin Yuan Qianglong Chen Jie Hu Jiaying Li Kexin Li Huan Wang Jinjin Ma Bin Meng Ruoxi Zhang Haijiao Mao Ting Liang Lijie Wang Chaoyong Liu Bin Li Fengxuan Han Programmed Transformation of Osteogenesis Microenvironment by a Multifunctional Hydrogel to Enhance Repair of Infectious Bone Defects Advanced Science antimicrobial property hydrogel infected bone defects melatonin carbon dot microenvironment osteogenesis |
| title | Programmed Transformation of Osteogenesis Microenvironment by a Multifunctional Hydrogel to Enhance Repair of Infectious Bone Defects |
| title_full | Programmed Transformation of Osteogenesis Microenvironment by a Multifunctional Hydrogel to Enhance Repair of Infectious Bone Defects |
| title_fullStr | Programmed Transformation of Osteogenesis Microenvironment by a Multifunctional Hydrogel to Enhance Repair of Infectious Bone Defects |
| title_full_unstemmed | Programmed Transformation of Osteogenesis Microenvironment by a Multifunctional Hydrogel to Enhance Repair of Infectious Bone Defects |
| title_short | Programmed Transformation of Osteogenesis Microenvironment by a Multifunctional Hydrogel to Enhance Repair of Infectious Bone Defects |
| title_sort | programmed transformation of osteogenesis microenvironment by a multifunctional hydrogel to enhance repair of infectious bone defects |
| topic | antimicrobial property hydrogel infected bone defects melatonin carbon dot microenvironment osteogenesis |
| url | https://doi.org/10.1002/advs.202409683 |
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