Anticancer Potential, Phenolic Profile, and Antioxidant Properties of <i>Synsepalum dulcificum</i> (Miracle Berry) in Colorectal Tumor Cell Lines
Polyphenols, recognized for their antioxidant capacity, have shown potential in improving the response treatment of various diseases, including cancer. In this context, polyphenols have the ability to induce cytotoxicity in tumor cells, making them possible complementary agents to current treatments...
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MDPI AG
2025-03-01
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| Series: | Antioxidants |
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| author | Josefa Quiroz-Troncoso Nicolás Alegría-Aravena Blanca Sáenz de Mierae Marta Sánchez-Díez Raquel González-Martos Clara E. Gavira-O’Neill Emilio J. González Maria González-Miquel Cristian Valdés Vergara Gloria González-Silva Loan Bensadon-Naeder Javier Galeano Carmen Ramírez-Castillejo |
| author_facet | Josefa Quiroz-Troncoso Nicolás Alegría-Aravena Blanca Sáenz de Mierae Marta Sánchez-Díez Raquel González-Martos Clara E. Gavira-O’Neill Emilio J. González Maria González-Miquel Cristian Valdés Vergara Gloria González-Silva Loan Bensadon-Naeder Javier Galeano Carmen Ramírez-Castillejo |
| author_sort | Josefa Quiroz-Troncoso |
| collection | DOAJ |
| description | Polyphenols, recognized for their antioxidant capacity, have shown potential in improving the response treatment of various diseases, including cancer. In this context, polyphenols have the ability to induce cytotoxicity in tumor cells, making them possible complementary agents to current treatments. The present study aims to evaluate the effect of the aqueous extract of <i>Synsepalum dulcificum</i>, using the commercial product DMB<sup>®</sup>, on the proliferation of colorectal tumor cells. An aqueous extract of DMB<sup>®</sup> was obtained, and 12 compounds were identified through high-performance liquid chromatography (HPLC), with protocatechuic acid, gallic acid, and catechin being the most prominent. Regarding cytotoxicity, the extracts reduced cell viability in the DLD-1, HT29, SW480, and SW620 cell lines, with IC<sub>50</sub> values of 7, 11, 13, and 15 mg/mL, respectively. The combination of oxaliplatin with the DMB<sup>®</sup> extract reduced the resistant population by up to 50% in the DLD-1 and SW620 cell lines, affecting the G<sub>2</sub>/M and S phases of the cell cycle, respectively. Additionally, treatment with the DMB<sup>®</sup> extract induced an increase in the expression of <i>BCL2</i>, <i>CASP3</i>, and <i>CASP9</i>, suggesting a mechanism of action associated with apoptosis. The aqueous extract of <i>Synsepalum dulcificum</i> (DMB<sup>®</sup>) exhibited cytotoxicity in colorectal cancer cells, enhancing the effect of oxaliplatin and activating apoptotic pathways, suggesting its potential as an adjuvant in anticancer therapies. |
| format | Article |
| id | doaj-art-e0a58bd8c9a04a6dba2ace97db05a7cc |
| institution | DOAJ |
| issn | 2076-3921 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
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| series | Antioxidants |
| spelling | doaj-art-e0a58bd8c9a04a6dba2ace97db05a7cc2025-08-20T03:14:24ZengMDPI AGAntioxidants2076-39212025-03-0114438110.3390/antiox14040381Anticancer Potential, Phenolic Profile, and Antioxidant Properties of <i>Synsepalum dulcificum</i> (Miracle Berry) in Colorectal Tumor Cell LinesJosefa Quiroz-Troncoso0Nicolás Alegría-Aravena1Blanca Sáenz de Mierae2Marta Sánchez-Díez3Raquel González-Martos4Clara E. Gavira-O’Neill5Emilio J. González6Maria González-Miquel7Cristian Valdés Vergara8Gloria González-Silva9Loan Bensadon-Naeder10Javier Galeano11Carmen Ramírez-Castillejo12HST Group, Centro de Tecnología Biomédica (CTB), Departamento Biotecnología-B.V. ETSIAAB, Universidad Politécnica de Madrid, 28223 Madrid, SpainDepartamento de Oncología, Instituto de Investigación Sanitaria San Carlos (IdISSC), 28040 Madrid, SpainDepartamento de Ingeniería Química Industrial y del Medio Ambiente, ETSI Industriales, Universidad Politécnica de Madrid, 28006 Madrid, SpainHST Group, Centro de Tecnología Biomédica (CTB), Departamento Biotecnología-B.V. ETSIAAB, Universidad Politécnica de Madrid, 28223 Madrid, SpainHST Group, Centro de Tecnología Biomédica (CTB), Departamento Biotecnología-B.V. ETSIAAB, Universidad Politécnica de Madrid, 28223 Madrid, SpainHST Group, Centro de Tecnología Biomédica (CTB), Departamento Biotecnología-B.V. ETSIAAB, Universidad Politécnica de Madrid, 28223 Madrid, SpainDepartamento de Ingeniería Química Industrial y del Medio Ambiente, ETSI Industriales, Universidad Politécnica de Madrid, 28006 Madrid, SpainDepartamento de Ingeniería Química Industrial y del Medio Ambiente, ETSI Industriales, Universidad Politécnica de Madrid, 28006 Madrid, SpainCentro de Investigación de Estudios Avanzados del Maule, Universidad Católica de Chile, Talca 3460000, ChileLaboratorio de Microbiología Aplicada, Escuela de Ingeniería en Biotecnología, Centro de Biotecnología de los Recursos Naturales (CenBio), Facultad de Ciencias Agrarias y Forestales, Universidad Católica del Maule, Talca 3466706, ChileMedicinal Gardens S.L. (Baïa Food), 28008 Madrid, SpainGrupo de Sistemas Complejos, Departamento de Ingeniería Agroforestal, ETSIAAB, Universidad Politécnica de Madrid, 28040 Madrid, SpainHST Group, Centro de Tecnología Biomédica (CTB), Departamento Biotecnología-B.V. ETSIAAB, Universidad Politécnica de Madrid, 28223 Madrid, SpainPolyphenols, recognized for their antioxidant capacity, have shown potential in improving the response treatment of various diseases, including cancer. In this context, polyphenols have the ability to induce cytotoxicity in tumor cells, making them possible complementary agents to current treatments. The present study aims to evaluate the effect of the aqueous extract of <i>Synsepalum dulcificum</i>, using the commercial product DMB<sup>®</sup>, on the proliferation of colorectal tumor cells. An aqueous extract of DMB<sup>®</sup> was obtained, and 12 compounds were identified through high-performance liquid chromatography (HPLC), with protocatechuic acid, gallic acid, and catechin being the most prominent. Regarding cytotoxicity, the extracts reduced cell viability in the DLD-1, HT29, SW480, and SW620 cell lines, with IC<sub>50</sub> values of 7, 11, 13, and 15 mg/mL, respectively. The combination of oxaliplatin with the DMB<sup>®</sup> extract reduced the resistant population by up to 50% in the DLD-1 and SW620 cell lines, affecting the G<sub>2</sub>/M and S phases of the cell cycle, respectively. Additionally, treatment with the DMB<sup>®</sup> extract induced an increase in the expression of <i>BCL2</i>, <i>CASP3</i>, and <i>CASP9</i>, suggesting a mechanism of action associated with apoptosis. The aqueous extract of <i>Synsepalum dulcificum</i> (DMB<sup>®</sup>) exhibited cytotoxicity in colorectal cancer cells, enhancing the effect of oxaliplatin and activating apoptotic pathways, suggesting its potential as an adjuvant in anticancer therapies.https://www.mdpi.com/2076-3921/14/4/381<i>Synsepalum dulcificum</i>antioxidant activityanticancer activitycolorectal cancerapoptotic pathwayscell cycle |
| spellingShingle | Josefa Quiroz-Troncoso Nicolás Alegría-Aravena Blanca Sáenz de Mierae Marta Sánchez-Díez Raquel González-Martos Clara E. Gavira-O’Neill Emilio J. González Maria González-Miquel Cristian Valdés Vergara Gloria González-Silva Loan Bensadon-Naeder Javier Galeano Carmen Ramírez-Castillejo Anticancer Potential, Phenolic Profile, and Antioxidant Properties of <i>Synsepalum dulcificum</i> (Miracle Berry) in Colorectal Tumor Cell Lines Antioxidants <i>Synsepalum dulcificum</i> antioxidant activity anticancer activity colorectal cancer apoptotic pathways cell cycle |
| title | Anticancer Potential, Phenolic Profile, and Antioxidant Properties of <i>Synsepalum dulcificum</i> (Miracle Berry) in Colorectal Tumor Cell Lines |
| title_full | Anticancer Potential, Phenolic Profile, and Antioxidant Properties of <i>Synsepalum dulcificum</i> (Miracle Berry) in Colorectal Tumor Cell Lines |
| title_fullStr | Anticancer Potential, Phenolic Profile, and Antioxidant Properties of <i>Synsepalum dulcificum</i> (Miracle Berry) in Colorectal Tumor Cell Lines |
| title_full_unstemmed | Anticancer Potential, Phenolic Profile, and Antioxidant Properties of <i>Synsepalum dulcificum</i> (Miracle Berry) in Colorectal Tumor Cell Lines |
| title_short | Anticancer Potential, Phenolic Profile, and Antioxidant Properties of <i>Synsepalum dulcificum</i> (Miracle Berry) in Colorectal Tumor Cell Lines |
| title_sort | anticancer potential phenolic profile and antioxidant properties of i synsepalum dulcificum i miracle berry in colorectal tumor cell lines |
| topic | <i>Synsepalum dulcificum</i> antioxidant activity anticancer activity colorectal cancer apoptotic pathways cell cycle |
| url | https://www.mdpi.com/2076-3921/14/4/381 |
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