Panaxatriol saponins exert anti-renal fibrosis by suppressing TNF-α mediated inflammation and TGF-β1/Smad3 signaling pathway
Renal fibrosis is a key pathological process in the progression of chronic kidney disease (CKD). Panaxatriol saponins (PTS), the main bioactive compounds extracted from Panax notoginseng (Burk.) F.H. Chen, have demonstrated antioxidative and anti-inflammatory activities. This study aimed to investig...
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| Format: | Article |
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Taylor & Francis Group
2025-12-01
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| Series: | Renal Failure |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/0886022X.2025.2516774 |
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| author | Si Yi Feiyan Li Xin Ma Yao Xu Weijing Lai Hongxi Chen Junming Fan Nan Mao Sichong Ren |
| author_facet | Si Yi Feiyan Li Xin Ma Yao Xu Weijing Lai Hongxi Chen Junming Fan Nan Mao Sichong Ren |
| author_sort | Si Yi |
| collection | DOAJ |
| description | Renal fibrosis is a key pathological process in the progression of chronic kidney disease (CKD). Panaxatriol saponins (PTS), the main bioactive compounds extracted from Panax notoginseng (Burk.) F.H. Chen, have demonstrated antioxidative and anti-inflammatory activities. This study aimed to investigate the potential protective effects of PTS against renal fibrosis and explore the underlying pharmacological mechanisms. A unilateral ureteral obstruction (UUO) model was established in Sprague-Dawley (SD) rats to induce renal fibrosis. Histopathological changes were assessed using hematoxylin and eosin (HE) staining, Masson’s trichrome staining, and transmission electron microscopy (TEM). Network pharmacology and molecular docking approaches were employed to identify potential signaling molecules through which PTS may mitigate renal fibrosis. Western blotting, quantitative real-time PCR (qRT-PCR), and immunohistochemistry were utilized to validate the involvement of specific signaling pathways in PTS-mediated anti-fibrotic effects. Our data demonstrated that PTS alleviated renal dysfunction and provided protective effects against renal fibrosis, primarily through the TNF-α and TGF-β1 signaling pathways. Moreover, PTS treatment significantly downregulated pro-inflammatory cytokines such as TNF-α, IL-6, and Smad3 activity. Additionally, PTS inhibited the expression of key fibrosis markers, including α-SMA, collagen I, and fibronectin. Our study suggests that PTS exert a prevention effect in renal fibrosis by blocking the TGF-β1/Smad3 signaling pathway. |
| format | Article |
| id | doaj-art-e0a521b697c848ecb9c46f92c4d22f13 |
| institution | Kabale University |
| issn | 0886-022X 1525-6049 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Renal Failure |
| spelling | doaj-art-e0a521b697c848ecb9c46f92c4d22f132025-08-20T03:30:45ZengTaylor & Francis GroupRenal Failure0886-022X1525-60492025-12-0147110.1080/0886022X.2025.2516774Panaxatriol saponins exert anti-renal fibrosis by suppressing TNF-α mediated inflammation and TGF-β1/Smad3 signaling pathwaySi Yi0Feiyan Li1Xin Ma2Yao Xu3Weijing Lai4Hongxi Chen5Junming Fan6Nan Mao7Sichong Ren8Department of Nephrology, The Second Affiliated Hospital of Chengdu Medical College, China National Nuclear Corporation 416 Hospital, Chengdu, ChinaClinical Medical College of Chengdu Medical College, Chengdu, ChinaClinical Medical College of Chengdu Medical College, Chengdu, ChinaClinical Medical College of Chengdu Medical College, Chengdu, ChinaClinical Medical College of Chengdu Medical College, Chengdu, ChinaClinical Medical College of Chengdu Medical College, Chengdu, ChinaClinical Medical College of Chengdu Medical College, Chengdu, ChinaClinical Medical College of Chengdu Medical College, Chengdu, ChinaClinical Medical College of Chengdu Medical College, Chengdu, ChinaRenal fibrosis is a key pathological process in the progression of chronic kidney disease (CKD). Panaxatriol saponins (PTS), the main bioactive compounds extracted from Panax notoginseng (Burk.) F.H. Chen, have demonstrated antioxidative and anti-inflammatory activities. This study aimed to investigate the potential protective effects of PTS against renal fibrosis and explore the underlying pharmacological mechanisms. A unilateral ureteral obstruction (UUO) model was established in Sprague-Dawley (SD) rats to induce renal fibrosis. Histopathological changes were assessed using hematoxylin and eosin (HE) staining, Masson’s trichrome staining, and transmission electron microscopy (TEM). Network pharmacology and molecular docking approaches were employed to identify potential signaling molecules through which PTS may mitigate renal fibrosis. Western blotting, quantitative real-time PCR (qRT-PCR), and immunohistochemistry were utilized to validate the involvement of specific signaling pathways in PTS-mediated anti-fibrotic effects. Our data demonstrated that PTS alleviated renal dysfunction and provided protective effects against renal fibrosis, primarily through the TNF-α and TGF-β1 signaling pathways. Moreover, PTS treatment significantly downregulated pro-inflammatory cytokines such as TNF-α, IL-6, and Smad3 activity. Additionally, PTS inhibited the expression of key fibrosis markers, including α-SMA, collagen I, and fibronectin. Our study suggests that PTS exert a prevention effect in renal fibrosis by blocking the TGF-β1/Smad3 signaling pathway.https://www.tandfonline.com/doi/10.1080/0886022X.2025.2516774Panaxatriol saponinschronic kidney diseaserenal fibrosisTNF-αTGF-β1/smad3UUO model |
| spellingShingle | Si Yi Feiyan Li Xin Ma Yao Xu Weijing Lai Hongxi Chen Junming Fan Nan Mao Sichong Ren Panaxatriol saponins exert anti-renal fibrosis by suppressing TNF-α mediated inflammation and TGF-β1/Smad3 signaling pathway Renal Failure Panaxatriol saponins chronic kidney disease renal fibrosis TNF-α TGF-β1/smad3 UUO model |
| title | Panaxatriol saponins exert anti-renal fibrosis by suppressing TNF-α mediated inflammation and TGF-β1/Smad3 signaling pathway |
| title_full | Panaxatriol saponins exert anti-renal fibrosis by suppressing TNF-α mediated inflammation and TGF-β1/Smad3 signaling pathway |
| title_fullStr | Panaxatriol saponins exert anti-renal fibrosis by suppressing TNF-α mediated inflammation and TGF-β1/Smad3 signaling pathway |
| title_full_unstemmed | Panaxatriol saponins exert anti-renal fibrosis by suppressing TNF-α mediated inflammation and TGF-β1/Smad3 signaling pathway |
| title_short | Panaxatriol saponins exert anti-renal fibrosis by suppressing TNF-α mediated inflammation and TGF-β1/Smad3 signaling pathway |
| title_sort | panaxatriol saponins exert anti renal fibrosis by suppressing tnf α mediated inflammation and tgf β1 smad3 signaling pathway |
| topic | Panaxatriol saponins chronic kidney disease renal fibrosis TNF-α TGF-β1/smad3 UUO model |
| url | https://www.tandfonline.com/doi/10.1080/0886022X.2025.2516774 |
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