TNFα-mediated liver destruction by Kupffer cells and Ly6Chi monocytes during Entamoeba histolytica infection.

Amebic liver abscess (ALA) is a focal destruction of liver tissue due to infection by the protozoan parasite Entamoeba histolytica (E. histolytica). Host tissue damage is attributed mainly to parasite pathogenicity factors, but massive early accumulation of mononuclear cells, including neutrophils,...

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Main Authors: Elena Helk, Hannah Bernin, Thomas Ernst, Harald Ittrich, Thomas Jacobs, Joerg Heeren, Frank Tacke, Egbert Tannich, Hannelore Lotter
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS Pathogens
Online Access:https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1003096&type=printable
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author Elena Helk
Hannah Bernin
Thomas Ernst
Harald Ittrich
Thomas Jacobs
Joerg Heeren
Frank Tacke
Egbert Tannich
Hannelore Lotter
author_facet Elena Helk
Hannah Bernin
Thomas Ernst
Harald Ittrich
Thomas Jacobs
Joerg Heeren
Frank Tacke
Egbert Tannich
Hannelore Lotter
author_sort Elena Helk
collection DOAJ
description Amebic liver abscess (ALA) is a focal destruction of liver tissue due to infection by the protozoan parasite Entamoeba histolytica (E. histolytica). Host tissue damage is attributed mainly to parasite pathogenicity factors, but massive early accumulation of mononuclear cells, including neutrophils, inflammatory monocytes and macrophages, at the site of infection raises the question of whether these cells also contribute to tissue damage. Using highly selective depletion strategies and cell-specific knockout mice, the relative contribution of innate immune cell populations to liver destruction during amebic infection was investigated. Neutrophils were not required for amebic infection nor did they appear to be substantially involved in tissue damage. In contrast, Kupffer cells and inflammatory monocytes contributed substantially to liver destruction during ALA, and tissue damage was mediated primarily by TNFα. These data indicate that besides direct antiparasitic drugs, modulating innate immune responses may potentially be beneficial in limiting ALA pathogenesis.
format Article
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institution OA Journals
issn 1553-7366
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language English
publishDate 2013-01-01
publisher Public Library of Science (PLoS)
record_format Article
series PLoS Pathogens
spelling doaj-art-e0a43c2f06314f318074b4a48be193842025-08-20T02:15:20ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742013-01-0191e100309610.1371/journal.ppat.1003096TNFα-mediated liver destruction by Kupffer cells and Ly6Chi monocytes during Entamoeba histolytica infection.Elena HelkHannah BerninThomas ErnstHarald IttrichThomas JacobsJoerg HeerenFrank TackeEgbert TannichHannelore LotterAmebic liver abscess (ALA) is a focal destruction of liver tissue due to infection by the protozoan parasite Entamoeba histolytica (E. histolytica). Host tissue damage is attributed mainly to parasite pathogenicity factors, but massive early accumulation of mononuclear cells, including neutrophils, inflammatory monocytes and macrophages, at the site of infection raises the question of whether these cells also contribute to tissue damage. Using highly selective depletion strategies and cell-specific knockout mice, the relative contribution of innate immune cell populations to liver destruction during amebic infection was investigated. Neutrophils were not required for amebic infection nor did they appear to be substantially involved in tissue damage. In contrast, Kupffer cells and inflammatory monocytes contributed substantially to liver destruction during ALA, and tissue damage was mediated primarily by TNFα. These data indicate that besides direct antiparasitic drugs, modulating innate immune responses may potentially be beneficial in limiting ALA pathogenesis.https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1003096&type=printable
spellingShingle Elena Helk
Hannah Bernin
Thomas Ernst
Harald Ittrich
Thomas Jacobs
Joerg Heeren
Frank Tacke
Egbert Tannich
Hannelore Lotter
TNFα-mediated liver destruction by Kupffer cells and Ly6Chi monocytes during Entamoeba histolytica infection.
PLoS Pathogens
title TNFα-mediated liver destruction by Kupffer cells and Ly6Chi monocytes during Entamoeba histolytica infection.
title_full TNFα-mediated liver destruction by Kupffer cells and Ly6Chi monocytes during Entamoeba histolytica infection.
title_fullStr TNFα-mediated liver destruction by Kupffer cells and Ly6Chi monocytes during Entamoeba histolytica infection.
title_full_unstemmed TNFα-mediated liver destruction by Kupffer cells and Ly6Chi monocytes during Entamoeba histolytica infection.
title_short TNFα-mediated liver destruction by Kupffer cells and Ly6Chi monocytes during Entamoeba histolytica infection.
title_sort tnfα mediated liver destruction by kupffer cells and ly6chi monocytes during entamoeba histolytica infection
url https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1003096&type=printable
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