Response of rhizobium to spent engine-oil contamination in the rhizosphere of legumes (arachis hypogaea and phaseolus vulgaris)

Abstract Indiscriminate releases of spent-engine oil into the environment portend danger to legume nodulation activities by Rhizobium. To ascertain this, response of Rhizobium to spent engine-oil in the rhizosphere of A. hypogaea and P. vulgaris were studied by growing viable seeds in spent engine...

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Main Authors: Monday Ubogu, Ejiro Akponah, Ejovwokoghene Francis Ejukonemu
Format: Article
Language:English
Published: Elsevier 2019-10-01
Series:Kuwait Journal of Science
Subjects:
Online Access:https://journalskuwait.org/kjs/index.php/KJS/article/view/4732
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author Monday Ubogu
Ejiro Akponah
Ejovwokoghene Francis Ejukonemu
author_facet Monday Ubogu
Ejiro Akponah
Ejovwokoghene Francis Ejukonemu
author_sort Monday Ubogu
collection DOAJ
description Abstract Indiscriminate releases of spent-engine oil into the environment portend danger to legume nodulation activities by Rhizobium. To ascertain this, response of Rhizobium to spent engine-oil in the rhizosphere of A. hypogaea and P. vulgaris were studied by growing viable seeds in spent engine-oil contaminated soil at 0, 1.0 and 3.0 % w/w for 120 days. Influence of oil on total populations of heterotrophs and Rhizobium in rhizosphere and nodule were estimated by soil dilution plate method on nutrient agar (NA) and yeast extract manitol agar (YEMA) respectively. Plant height and root growth assessed using graduated metre rule; nodule and pod numbers by counting. Results showed population of Rhizobium in rhizosphere and nodule decreased with increased oil concentrations in contrast to observed increases in bacterial heterotrophs for tested legumes (P ˂ 0.05). Plant height, root length, nodule and pod formation decreased with increased oil concentrations in soil (P ˂ 0.05). Spent engine-oil impeded Rhizobium response in rhizosphere of legumes.
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issn 2307-4108
2307-4116
language English
publishDate 2019-10-01
publisher Elsevier
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series Kuwait Journal of Science
spelling doaj-art-e09fd944d5b948ea84a20796393b486d2025-08-20T03:09:47ZengElsevierKuwait Journal of Science2307-41082307-41162019-10-01464Response of rhizobium to spent engine-oil contamination in the rhizosphere of legumes (arachis hypogaea and phaseolus vulgaris)Monday Ubogu0Ejiro Akponah1Ejovwokoghene Francis Ejukonemu2Federal University of Agriculture MakurdiDelta State University, AbrakaDelta State Polytechnic, Oghara. Abstract Indiscriminate releases of spent-engine oil into the environment portend danger to legume nodulation activities by Rhizobium. To ascertain this, response of Rhizobium to spent engine-oil in the rhizosphere of A. hypogaea and P. vulgaris were studied by growing viable seeds in spent engine-oil contaminated soil at 0, 1.0 and 3.0 % w/w for 120 days. Influence of oil on total populations of heterotrophs and Rhizobium in rhizosphere and nodule were estimated by soil dilution plate method on nutrient agar (NA) and yeast extract manitol agar (YEMA) respectively. Plant height and root growth assessed using graduated metre rule; nodule and pod numbers by counting. Results showed population of Rhizobium in rhizosphere and nodule decreased with increased oil concentrations in contrast to observed increases in bacterial heterotrophs for tested legumes (P ˂ 0.05). Plant height, root length, nodule and pod formation decreased with increased oil concentrations in soil (P ˂ 0.05). Spent engine-oil impeded Rhizobium response in rhizosphere of legumes. https://journalskuwait.org/kjs/index.php/KJS/article/view/4732ContaminationlegumeRhizobiumrhizospherenodulespent engine-oil
spellingShingle Monday Ubogu
Ejiro Akponah
Ejovwokoghene Francis Ejukonemu
Response of rhizobium to spent engine-oil contamination in the rhizosphere of legumes (arachis hypogaea and phaseolus vulgaris)
Kuwait Journal of Science
Contamination
legume
Rhizobium
rhizosphere
nodule
spent engine-oil
title Response of rhizobium to spent engine-oil contamination in the rhizosphere of legumes (arachis hypogaea and phaseolus vulgaris)
title_full Response of rhizobium to spent engine-oil contamination in the rhizosphere of legumes (arachis hypogaea and phaseolus vulgaris)
title_fullStr Response of rhizobium to spent engine-oil contamination in the rhizosphere of legumes (arachis hypogaea and phaseolus vulgaris)
title_full_unstemmed Response of rhizobium to spent engine-oil contamination in the rhizosphere of legumes (arachis hypogaea and phaseolus vulgaris)
title_short Response of rhizobium to spent engine-oil contamination in the rhizosphere of legumes (arachis hypogaea and phaseolus vulgaris)
title_sort response of rhizobium to spent engine oil contamination in the rhizosphere of legumes arachis hypogaea and phaseolus vulgaris
topic Contamination
legume
Rhizobium
rhizosphere
nodule
spent engine-oil
url https://journalskuwait.org/kjs/index.php/KJS/article/view/4732
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