Research on the Cryopreservation System of Controlled Rapid Cooling and Rewarming of Cellular Micro-droplets
The vitrification preservation method of cell microdroplets has received extensive attention in the field of cryopreservation. However, the vitrification process of microdroplets still has limitations such as uncontrollable cooling/rewarming processes. In this paper, a cryopreservation system for co...
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| Format: | Article |
| Language: | zho |
| Published: |
Journal of Refrigeration Magazines Agency Co., Ltd.
2025-01-01
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| Series: | Zhileng xuebao |
| Subjects: | |
| Online Access: | http://www.zhilengxuebao.com/thesisDetails?columnId=84622420&Fpath=home&index=0 |
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| Summary: | The vitrification preservation method of cell microdroplets has received extensive attention in the field of cryopreservation. However, the vitrification process of microdroplets still has limitations such as uncontrollable cooling/rewarming processes. In this paper, a cryopreservation system for controllable rapid cooling and rewarming during the vitrification process of cell microdroplets was constructed by combining the lifting of a moving slide table with the Joule heating rewarming method. This system achieves rapid and controllable cooling by adjusting the speed of immersion in liquid nitrogen, and realizes a high-rate rewarming process by controlling the heating time and current intensity of Joule heating, thus realizing the vitrification process of microdroplets and significantly avoiding the damage to cells caused by devitrification during the droplet rewarming process. The results show that the cooling rate controlled by this system can reach 1.8 × 10⁴ °C/min, enabling the vitrification preservation of cell microdroplets with a relatively low concentration of cryoprotectant. The rewarming rate control can reach 4.0 × 10⁴ °C/min, which can effectively avoid the occurrence of devitrification and ice crystal regrowth during the rewarming process of the relatively low concentration of cryoprotectant. Through the cryopreservation effect of A549 cells in microdroplets, it has been confirmed that the survival rate after cryopreservation and resuscitation of this system is significantly higher than that of the traditional straw preservation. The research work in this paper is expected to provide new solutions for the automatic vitrification preservation and rewarming methods of cell microdroplets. |
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| ISSN: | 0253-4339 |