The role and mechanism of long non-coding RNA ARID2-IR in chronic glomerulonephritis
Objective To investigate the relationship between LncRNA ARID2-IR and renal function in patients with chronic glomerulonephritis(CGN)and its mechanism.Methods A total of 52 CGN patients(CGN group)and 45 healthy adults(control group)were enrolled in the study.General information(age,gender,blood pres...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Editorial Department of Journal of Clinical Nephrology
2020-01-01
|
| Series: | Linchuang shenzangbing zazhi |
| Subjects: | |
| Online Access: | http://www.lcszb.com/thesisDetails?columnId=57907223&Fpath=home&index=0 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849729351114817536 |
|---|---|
| author | ZHANG Ai-ping HE Fang SHE Kai-ru |
| author_facet | ZHANG Ai-ping HE Fang SHE Kai-ru |
| author_sort | ZHANG Ai-ping |
| collection | DOAJ |
| description | Objective To investigate the relationship between LncRNA ARID2-IR and renal function in patients with chronic glomerulonephritis(CGN)and its mechanism.Methods A total of 52 CGN patients(CGN group)and 45 healthy adults(control group)were enrolled in the study.General information(age,gender,blood pressure,body mass index,etc.)was collected.Blood urea nitrogen(BUN)and serum creatinine(Scr)were tested.Target gene prediction was performed through the Starbase database,and KEGG pathway enrichment analysis was performed by Tbtools software.Real-time fluorescent quantitative PCR was used to detect the expression of LncRNA ARID2-IR and target genes.With ROC curve,the diagnostic value of LncRNA ARID2-IR and target genes for CGN was analyzed,and the influencing factors of CGN were analyzed by Logistic regression model.Results Systolic blood pressure,diastolic blood pressure,BUN and Scr in the CGN group were significantly higher than those in the control group(P<0.05).The expression of LncRNA ARID2-IR in the CGN group was significantly higher than that in the control group(P<0.05).LncRNA ARID2-IR expression was significantly correlated with BUN(r=0.225,P<0.05)and Scr(r=0.266,P<0.05),respectively.Target gene prediction showed that LncRNA ARID2-IR had 88 target genes in total,and the top 5 target genes in the score ranking for matching with LncRNA ARID2-IR were IFT80,IGHG4,RLBP1,ANKRD27 and TSPAN6,of which IFT80 and RLBP1 in the CGN group had much higher expression levels than those in the control group(P<0.05).The ROC curve showed that the AUCs of LncRNA ARID2-IR,IFT80 and RLBP1 were 0.783,0.821 and 0.738,respectively,which was of diagnostic significance for CGN.Logistic regression analysis showed that elevated levels of LncRNA ARID2-IR(OR=4.754,P=0.021),IFT80(OR=3.518,P=0.001)and RLBP1(OR=4.718,P=0.021)increased CGN risk.Conclusions Up-regulation of LncRNA ARID2-IR expression plays an important role in CGN.LncRNA ARID2-IR may be a new target for CGN control. |
| format | Article |
| id | doaj-art-e09a415d00cb4f87a3b4ee2ebba5b807 |
| institution | DOAJ |
| issn | 1671-2390 |
| language | zho |
| publishDate | 2020-01-01 |
| publisher | Editorial Department of Journal of Clinical Nephrology |
| record_format | Article |
| series | Linchuang shenzangbing zazhi |
| spelling | doaj-art-e09a415d00cb4f87a3b4ee2ebba5b8072025-08-20T03:09:15ZzhoEditorial Department of Journal of Clinical NephrologyLinchuang shenzangbing zazhi1671-23902020-01-012078679157907223The role and mechanism of long non-coding RNA ARID2-IR in chronic glomerulonephritisZHANG Ai-pingHE FangSHE Kai-ruObjective To investigate the relationship between LncRNA ARID2-IR and renal function in patients with chronic glomerulonephritis(CGN)and its mechanism.Methods A total of 52 CGN patients(CGN group)and 45 healthy adults(control group)were enrolled in the study.General information(age,gender,blood pressure,body mass index,etc.)was collected.Blood urea nitrogen(BUN)and serum creatinine(Scr)were tested.Target gene prediction was performed through the Starbase database,and KEGG pathway enrichment analysis was performed by Tbtools software.Real-time fluorescent quantitative PCR was used to detect the expression of LncRNA ARID2-IR and target genes.With ROC curve,the diagnostic value of LncRNA ARID2-IR and target genes for CGN was analyzed,and the influencing factors of CGN were analyzed by Logistic regression model.Results Systolic blood pressure,diastolic blood pressure,BUN and Scr in the CGN group were significantly higher than those in the control group(P<0.05).The expression of LncRNA ARID2-IR in the CGN group was significantly higher than that in the control group(P<0.05).LncRNA ARID2-IR expression was significantly correlated with BUN(r=0.225,P<0.05)and Scr(r=0.266,P<0.05),respectively.Target gene prediction showed that LncRNA ARID2-IR had 88 target genes in total,and the top 5 target genes in the score ranking for matching with LncRNA ARID2-IR were IFT80,IGHG4,RLBP1,ANKRD27 and TSPAN6,of which IFT80 and RLBP1 in the CGN group had much higher expression levels than those in the control group(P<0.05).The ROC curve showed that the AUCs of LncRNA ARID2-IR,IFT80 and RLBP1 were 0.783,0.821 and 0.738,respectively,which was of diagnostic significance for CGN.Logistic regression analysis showed that elevated levels of LncRNA ARID2-IR(OR=4.754,P=0.021),IFT80(OR=3.518,P=0.001)and RLBP1(OR=4.718,P=0.021)increased CGN risk.Conclusions Up-regulation of LncRNA ARID2-IR expression plays an important role in CGN.LncRNA ARID2-IR may be a new target for CGN control.http://www.lcszb.com/thesisDetails?columnId=57907223&Fpath=home&index=0ChronicGlomerulonephritisLong non-coding RNAARID2-IRTarget gene |
| spellingShingle | ZHANG Ai-ping HE Fang SHE Kai-ru The role and mechanism of long non-coding RNA ARID2-IR in chronic glomerulonephritis Linchuang shenzangbing zazhi Chronic Glomerulonephritis Long non-coding RNA ARID2-IR Target gene |
| title | The role and mechanism of long non-coding RNA ARID2-IR in chronic glomerulonephritis |
| title_full | The role and mechanism of long non-coding RNA ARID2-IR in chronic glomerulonephritis |
| title_fullStr | The role and mechanism of long non-coding RNA ARID2-IR in chronic glomerulonephritis |
| title_full_unstemmed | The role and mechanism of long non-coding RNA ARID2-IR in chronic glomerulonephritis |
| title_short | The role and mechanism of long non-coding RNA ARID2-IR in chronic glomerulonephritis |
| title_sort | role and mechanism of long non coding rna arid2 ir in chronic glomerulonephritis |
| topic | Chronic Glomerulonephritis Long non-coding RNA ARID2-IR Target gene |
| url | http://www.lcszb.com/thesisDetails?columnId=57907223&Fpath=home&index=0 |
| work_keys_str_mv | AT zhangaiping theroleandmechanismoflongnoncodingrnaarid2irinchronicglomerulonephritis AT hefang theroleandmechanismoflongnoncodingrnaarid2irinchronicglomerulonephritis AT shekairu theroleandmechanismoflongnoncodingrnaarid2irinchronicglomerulonephritis AT zhangaiping roleandmechanismoflongnoncodingrnaarid2irinchronicglomerulonephritis AT hefang roleandmechanismoflongnoncodingrnaarid2irinchronicglomerulonephritis AT shekairu roleandmechanismoflongnoncodingrnaarid2irinchronicglomerulonephritis |