Metabolomics-based study on the significance of differential metabolite binding IgG isoforms in Hemolytic disease of newborn
Background: Hemolytic disease of the newborn (HDN) is a common condition that can have a severe impact on the health of newborns due to the hemolytic reactions it triggers. Although numerous studies have focused on understanding the pathogenesis of HDN, there are still many unanswered questions.Meth...
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Taylor & Francis Group
2024-12-01
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| Series: | Hematology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/16078454.2024.2360339 |
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| author | Shipeng Zhang Sijin Li Xuan Meng Jia Chen Yan Tang Xiaobin Li |
| author_facet | Shipeng Zhang Sijin Li Xuan Meng Jia Chen Yan Tang Xiaobin Li |
| author_sort | Shipeng Zhang |
| collection | DOAJ |
| description | Background: Hemolytic disease of the newborn (HDN) is a common condition that can have a severe impact on the health of newborns due to the hemolytic reactions it triggers. Although numerous studies have focused on understanding the pathogenesis of HDN, there are still many unanswered questions.Methods: In this retrospective study, serum samples were collected from 15 healthy newborns and 8 infants diagnosed with hemolytic disease. The relationship between different metabolites and various IgG subtypes in Healthy, HDN and BLI groups was studied by biochemical technique and enzyme-linked immunosorbent assay (ELISA). Metabolomics analysis was conducted to identify the differential metabolites associated with HDN. Subsequently, Pearson's correlation analysis was used to determine the relation of these differential metabolites with IgG isoforms. The relationship between the metabolites and IgG subtypes was observed after treatment.Results: The study results revealed that infants with hemolytic disease exhibited abnormal elevations in TBA, IgG1, IgG2a, IgG2b, IgG3, and IgG4 levels when compared to healthy newborns. Additionally, differences in metabolite contents were also observed. N, N-DIMETHYLARGININE showed negative correlations with TBA, IgG1, IgG2a, IgG2b, IgG3, and IgG4, while 2-HYDROXYBUTYRATE, AMINOISOBUTANOATE, Inosine, and ALLYL ISOTHIOCYANATE exhibited positive correlations with TBA, IgG1, IgG2a, IgG2b, IgG3, and IgG4. Through metabolomics-based research, we have discovered associations between differential metabolites and different IgG isoforms during the onset of HDN.Conclusion: These findings suggest that changes in metabolite and IgG isoform levels are linked to HDN. Understanding the involvement of IgG isoforms and metabolites can provide valuable guidance for the diagnosis and treatment of HDN. |
| format | Article |
| id | doaj-art-e095cc408b934d4fb15b772c1fdace0f |
| institution | DOAJ |
| issn | 1607-8454 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Hematology |
| spelling | doaj-art-e095cc408b934d4fb15b772c1fdace0f2025-08-20T02:50:19ZengTaylor & Francis GroupHematology1607-84542024-12-0129110.1080/16078454.2024.2360339Metabolomics-based study on the significance of differential metabolite binding IgG isoforms in Hemolytic disease of newbornShipeng Zhang0Sijin Li1Xuan Meng2Jia Chen3Yan Tang4Xiaobin Li5Zhuzhou 331 hospital, Zhuzhou, People’s Republic of ChinaZhuzhou 331 hospital, Zhuzhou, People’s Republic of ChinaZhuzhou 331 hospital, Zhuzhou, People’s Republic of ChinaZhuzhou 331 hospital, Zhuzhou, People’s Republic of ChinaZhuzhou 331 hospital, Zhuzhou, People’s Republic of ChinaZhuzhou 331 hospital, Zhuzhou, People’s Republic of ChinaBackground: Hemolytic disease of the newborn (HDN) is a common condition that can have a severe impact on the health of newborns due to the hemolytic reactions it triggers. Although numerous studies have focused on understanding the pathogenesis of HDN, there are still many unanswered questions.Methods: In this retrospective study, serum samples were collected from 15 healthy newborns and 8 infants diagnosed with hemolytic disease. The relationship between different metabolites and various IgG subtypes in Healthy, HDN and BLI groups was studied by biochemical technique and enzyme-linked immunosorbent assay (ELISA). Metabolomics analysis was conducted to identify the differential metabolites associated with HDN. Subsequently, Pearson's correlation analysis was used to determine the relation of these differential metabolites with IgG isoforms. The relationship between the metabolites and IgG subtypes was observed after treatment.Results: The study results revealed that infants with hemolytic disease exhibited abnormal elevations in TBA, IgG1, IgG2a, IgG2b, IgG3, and IgG4 levels when compared to healthy newborns. Additionally, differences in metabolite contents were also observed. N, N-DIMETHYLARGININE showed negative correlations with TBA, IgG1, IgG2a, IgG2b, IgG3, and IgG4, while 2-HYDROXYBUTYRATE, AMINOISOBUTANOATE, Inosine, and ALLYL ISOTHIOCYANATE exhibited positive correlations with TBA, IgG1, IgG2a, IgG2b, IgG3, and IgG4. Through metabolomics-based research, we have discovered associations between differential metabolites and different IgG isoforms during the onset of HDN.Conclusion: These findings suggest that changes in metabolite and IgG isoform levels are linked to HDN. Understanding the involvement of IgG isoforms and metabolites can provide valuable guidance for the diagnosis and treatment of HDN.https://www.tandfonline.com/doi/10.1080/16078454.2024.2360339HDNTBAIgGmetabolomics |
| spellingShingle | Shipeng Zhang Sijin Li Xuan Meng Jia Chen Yan Tang Xiaobin Li Metabolomics-based study on the significance of differential metabolite binding IgG isoforms in Hemolytic disease of newborn Hematology HDN TBA IgG metabolomics |
| title | Metabolomics-based study on the significance of differential metabolite binding IgG isoforms in Hemolytic disease of newborn |
| title_full | Metabolomics-based study on the significance of differential metabolite binding IgG isoforms in Hemolytic disease of newborn |
| title_fullStr | Metabolomics-based study on the significance of differential metabolite binding IgG isoforms in Hemolytic disease of newborn |
| title_full_unstemmed | Metabolomics-based study on the significance of differential metabolite binding IgG isoforms in Hemolytic disease of newborn |
| title_short | Metabolomics-based study on the significance of differential metabolite binding IgG isoforms in Hemolytic disease of newborn |
| title_sort | metabolomics based study on the significance of differential metabolite binding igg isoforms in hemolytic disease of newborn |
| topic | HDN TBA IgG metabolomics |
| url | https://www.tandfonline.com/doi/10.1080/16078454.2024.2360339 |
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