Enhancement of Macrophage Function by the Antimicrobial Peptide Sublancin Protects Mice from Methicillin-Resistant Staphylococcus aureus
Methicillin-resistant Staphylococcus aureus (MRSA) is the major pathogen responsible for community and hospital bacterial infections. Sublancin, a glucosylated antimicrobial peptide isolated from Bacillus subtilis 168, possesses antibacterial infective effects. In this study, we investigated the rol...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2019/3979352 |
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| author | Shuai Wang Qianhong Ye Ke Wang Xiangfang Zeng Shuo Huang Haitao Yu Qing Ge Desheng Qi Shiyan Qiao |
| author_facet | Shuai Wang Qianhong Ye Ke Wang Xiangfang Zeng Shuo Huang Haitao Yu Qing Ge Desheng Qi Shiyan Qiao |
| author_sort | Shuai Wang |
| collection | DOAJ |
| description | Methicillin-resistant Staphylococcus aureus (MRSA) is the major pathogen responsible for community and hospital bacterial infections. Sublancin, a glucosylated antimicrobial peptide isolated from Bacillus subtilis 168, possesses antibacterial infective effects. In this study, we investigated the role and anti-infection mechanism of sublancin in a mouse model of MRSA-induced sublethal infection. Sublancin could modulate innate immunity by inducing the production of IL-1β, IL-6, TNF-α, and nitric oxide, enhancing phagocytosis and MRSA-killing activity in both RAW264.7 cells and mouse peritoneal macrophages. The enhanced macrophage function by the peptide in vitro correlated with stronger protective activity in vivo in the MRSA-invasive sublethal infection model. Macrophage activation by sublancin was found to be partly dependent on TLR4 and the NF-κB and MAPK signaling pathways. Moreover, oral administration of sublancin increased the frequencies of CD4+ and CD8+ T cells in mesenteric lymph nodes. The protective activity of sublancin was associated with in vivo augmenting phagocytic activity of peritoneal macrophages and partly improving T cell-mediated immunity. Macrophages thus represent a potentially pivotal and novel target for future development of innate defense regulator therapeutics against S. aureus infection. |
| format | Article |
| id | doaj-art-e095631aa2d449b7ae30a21b214c8ef6 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-e095631aa2d449b7ae30a21b214c8ef62025-08-20T03:26:00ZengWileyJournal of Immunology Research2314-88612314-71562019-01-01201910.1155/2019/39793523979352Enhancement of Macrophage Function by the Antimicrobial Peptide Sublancin Protects Mice from Methicillin-Resistant Staphylococcus aureusShuai Wang0Qianhong Ye1Ke Wang2Xiangfang Zeng3Shuo Huang4Haitao Yu5Qing Ge6Desheng Qi7Shiyan Qiao8State Key Laboratory of Animal Nutrition, Beijing Key Laboratory of Biofeed Additives, Ministry of Agriculture Feed Industry Center, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Beijing Key Laboratory of Biofeed Additives, Ministry of Agriculture Feed Industry Center, China Agricultural University, Beijing 100193, ChinaKey Laboratory of Medical Immunology, Ministry of Health, Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, ChinaState Key Laboratory of Animal Nutrition, Beijing Key Laboratory of Biofeed Additives, Ministry of Agriculture Feed Industry Center, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Beijing Key Laboratory of Biofeed Additives, Ministry of Agriculture Feed Industry Center, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Beijing Key Laboratory of Biofeed Additives, Ministry of Agriculture Feed Industry Center, China Agricultural University, Beijing 100193, ChinaKey Laboratory of Medical Immunology, Ministry of Health, Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, ChinaDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaState Key Laboratory of Animal Nutrition, Beijing Key Laboratory of Biofeed Additives, Ministry of Agriculture Feed Industry Center, China Agricultural University, Beijing 100193, ChinaMethicillin-resistant Staphylococcus aureus (MRSA) is the major pathogen responsible for community and hospital bacterial infections. Sublancin, a glucosylated antimicrobial peptide isolated from Bacillus subtilis 168, possesses antibacterial infective effects. In this study, we investigated the role and anti-infection mechanism of sublancin in a mouse model of MRSA-induced sublethal infection. Sublancin could modulate innate immunity by inducing the production of IL-1β, IL-6, TNF-α, and nitric oxide, enhancing phagocytosis and MRSA-killing activity in both RAW264.7 cells and mouse peritoneal macrophages. The enhanced macrophage function by the peptide in vitro correlated with stronger protective activity in vivo in the MRSA-invasive sublethal infection model. Macrophage activation by sublancin was found to be partly dependent on TLR4 and the NF-κB and MAPK signaling pathways. Moreover, oral administration of sublancin increased the frequencies of CD4+ and CD8+ T cells in mesenteric lymph nodes. The protective activity of sublancin was associated with in vivo augmenting phagocytic activity of peritoneal macrophages and partly improving T cell-mediated immunity. Macrophages thus represent a potentially pivotal and novel target for future development of innate defense regulator therapeutics against S. aureus infection.http://dx.doi.org/10.1155/2019/3979352 |
| spellingShingle | Shuai Wang Qianhong Ye Ke Wang Xiangfang Zeng Shuo Huang Haitao Yu Qing Ge Desheng Qi Shiyan Qiao Enhancement of Macrophage Function by the Antimicrobial Peptide Sublancin Protects Mice from Methicillin-Resistant Staphylococcus aureus Journal of Immunology Research |
| title | Enhancement of Macrophage Function by the Antimicrobial Peptide Sublancin Protects Mice from Methicillin-Resistant Staphylococcus aureus |
| title_full | Enhancement of Macrophage Function by the Antimicrobial Peptide Sublancin Protects Mice from Methicillin-Resistant Staphylococcus aureus |
| title_fullStr | Enhancement of Macrophage Function by the Antimicrobial Peptide Sublancin Protects Mice from Methicillin-Resistant Staphylococcus aureus |
| title_full_unstemmed | Enhancement of Macrophage Function by the Antimicrobial Peptide Sublancin Protects Mice from Methicillin-Resistant Staphylococcus aureus |
| title_short | Enhancement of Macrophage Function by the Antimicrobial Peptide Sublancin Protects Mice from Methicillin-Resistant Staphylococcus aureus |
| title_sort | enhancement of macrophage function by the antimicrobial peptide sublancin protects mice from methicillin resistant staphylococcus aureus |
| url | http://dx.doi.org/10.1155/2019/3979352 |
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